tt2015

Targeting Trends 2015 Newsletter

2015, issue 1: Impairments in gait, posture and complex movement control in rats modeling the multi-system, cholinergic-dopaminergic losses in Parkinson’s Disease / by Aaron Kucinski / featuring 192-IgG-SAP (Cat. #IT-01), Anti-ChAT-SAP (Cat. #IT-42) Society for Neuroscience Poster of the Year Award Veterinary Development of Substance P-Saporin (SP-SAP) Targeting Talk: Protocols and Dosage Targeting Tools: ZAP SrB Development …

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Targeting Tools: MonoBiotin-ZAP

The conjugation specialists at Advanced Targeting Systems are proud to announce a new addition to the catalog: MonoBiotin-ZAP Cat. #BT-ZAP Researchers now have more freedom in selecting conjugation strategies. Users have taken advantage of the ability to use biotinylated targeting agents with Streptavidin-ZAP (Cat. #IT-27) a chemical conjugate between saporin and streptavidin. Streptavidin-ZAP converts biotinylated …

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Targeting Tools: Anti-ChAT-SAP

Choline acetyltransferase (ChAT) catalyzes the synthesis of the neurotransmitter acetylcholine (ACh) from choline and acetyl-CoA in cholinergic neurons. ChAT serves as a specific marker for cholinergic neurons in both peripheral and central nervous systems. Evidence shows that ChAT exists in two forms inside cholinergic nerve terminals, a soluble hydrophilic form and the membrane-associated amphiphilic form.[1-2] …

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Targeting Topics 15q4

Alterations in the rostral ventromedial medulla after the selective ablation of mu-opioid receptor expressing neurons. Harasawa I, Johansen JP, Fields HL, Porreca F, Meng ID. Pain 157(1):166-173, 2016. The rostral ventromedial medulla (RVM) has both excitatory and inhibitory control over nociceptive neurons in the medullary dorsal horn and spinal cord. Previous work has demonstrated that …

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Cover Article: Anti-ChAT-SAP elucidates a causal role in behavioral flexibility

A specific immunotoxin elucidates a causal role of striatal cholinergic system in behavioral flexibility by Sho Aoki and Jeffery R. Wickens Neurobiology Research Unit, Okinawa Institute of Science and Technology, 1919-1 Tancha, Onna, Kunigami, Okinawa 904-0495, Japan Behavioral flexibility is broadly defined as the ability to change behavioral strategy, according to a change of governing …

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Targeting Talk: Using Kit Controls

Q: Your targeted toxin kits come with different controls. I’m not sure of the best way to use them; there is included unconjugated antibody, unconjugated saporin, and a control conjugate, mouse IgG-SAP. Should I use them all in the same experiment or for different purposes? A: For mouse IgG-containing conjugates, the ideal control is Mouse …

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Targeting Topics 15q3

Targeted ablation of cholinergic interneurons in the dorsolateral striatum produces behavioral manifestations of Tourette syndrome. Xu M, Kobets A, Du JC, Lennington J, Li L, Banasr M, Duman RS, Vaccarino FM, DiLeone RJ, Pittenger C. Proc Natl Acad Sci U S A 112(3):893-898, 2015. Postmortem studies of Tourette syndrome patients has revealed a reduction in …

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