Anti-vGAT-SAP [IT-71, KIT-71]

a tool for eliminating cells that express express the vesicular GABA transporter; targeted via affinity-purified anti-vGAT, eliminated via saporin

SKU: IT-71 Category: Quantity: Individual 25 ug, Individual 100 ug, Individual 250 ug, Individual 1 mg, Kit w/controls 25 ug, Kit w/controls 100 ug, Kit w/controls 250 ug | Antibody Type: affinity-purified, Polyclonal | Host: rabbit | Reactivity: guinea pig, human, mouse, pig, rat | Conjugate: streptavidin-saporin | Usage: eliminates cells, retrograde transport |

Vesicular GABA transporters (vGAT) mediate the accumulation of GABA into synaptic vesicles and its release from nerve terminals. This transporter is expressed in the nerve endings of GABAergic neurons throughout the CNS and has also been found in the pancreas and pituitary gland. During development, expression of the vGAT protein changes. Expression can also change in response to patterns of neuronal activity.

Anti-vGAT-SAP is a bonded toxin between a biotinylated rabbit polyclonal antibody and the secondary conjugate Streptavidin-ZAP containing the ribosome-inactivating protein, saporin. It specifically eliminates cells that express the vesicular GABA transporter.

Anti-vGAT-SAP is available individually (Cat. #IT-71) or as a kit (Cat. #KIT-71) which includes Anti-vGAT-SAP and BIgG-SAP Rabbit (Cat. #IT-75).

keywords: Vesicular GABA transporter, vGAT, Anti-vGAT, Anti-Vescular GABA Transporter, saporin, GABAergic neurons, brain, neuroscience

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Oscillatory coupling within neonatal prefrontal-hippocampal networks is independent of selective removal of GABAergic neurons in the hippocampus.

Bitzenhofer SH, Hanganu-Opatz IL (2014) Oscillatory coupling within neonatal prefrontal-hippocampal networks is independent of selective removal of GABAergic neurons in the hippocampus. Neuropharmacology 77:57-67. doi: 10.1016/j.neuropharm.2013.09.007 PMID: 24056266

Summary: During cognitive tasks neuronal networks are entrained by oscillatory electrical rhythms with different frequencies. It has been proposed that GABAergic neurons in the prefrontal-hippocampal networks control this processing. The authors administered 252 ng of Anti-vGAT-SAP (Cat. #IT-71) into the ventral hippocampus of rats to examine how the GABAergic neurons could be involved. Unconjugated anti-vGAT (Cat #AB-N44) was used as a control. Hippocampal sharp waves were impaired during neonatal development, but the data indicate that oscillatory coupling between the neonatal prefrontal cortex and hippocampus is not controlled by GABAergic hippocampal interneurons.

Related Products: Anti-vGAT-SAP (Cat. #IT-71), vGAT Rabbit Polyclonal (Cat. #AB-N44)

Cracking down on inhibition: Selective removal of GABAergic interneurons from hippocampal networks.

Antonucci F, Alpár A, Kacza J, Caleo M, Verderio C, Giani A, Martens H, Chaudhry FA, Allegra M, Grosche J, Michalski D, Erck C, Hoffmann A, Harkany T, Matteoli M, Härtig W (2012) Cracking down on inhibition: Selective removal of GABAergic interneurons from hippocampal networks. J Neurosci 32(6):1989-2001. doi: 10.1523/JNEUROSCI.2720-11.2012

Related Products: Anti-vGAT-SAP (Cat. #IT-71)

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BIgG-SAP Rabbit (Cat. #IT-75)

vGAT Products:

vGAT Rabbit Polyclonal (Cat. #AB-N44) | affinity-purified (Cat. #AB-N44AP) | affinity-purified biotin-labeled (Cat. #AB-N44AP-BT) | affinity-purified Alexa488-labeled (Cat. #AB-N44AP-FLA)

Anti-vGAT-SAP (Cat. #IT-71)

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Toolbox: vGAT Products

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Cytotoxicity Assay for Targeted Toxins in vitro

Concentration Calculation Explained: Convert molarity to mg/ml and mg/ml to molarity

Preparing and Interpreting Cytotoxicity Data in vitro


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