ATS is pleased to present a new product line specific for the vesicular GABA transporter (vGAT) protein. vGAT mediates both the accumulaXon of GABA into synaptic vesicles and its release from nerve terminals. vGAT is expressed in nerve endings of GABAergic neurons throughout the CNS. The GABAergic system is crucial to the development and functional maturation of the nervous system, as well as the maintenance of balance between excitation and inhibition required for normal neural circuit function. Dysfunction of GABAergic neurons underlies aspects of clinical symptoms found in several diseases such as epilepsy, Down Syndrome, Fragile X Syndrome, Schizophrenia, and Autism among others.
Anti-vGAT-SAP (Cat. #IT-71) is highly specific for cells that express vGAT. Instead of spending precious time and money producing a vGAT knockout animal, you can use Anti-vGAT-SAP to specifically eliminate cells that express vGAT. Anti-vGAT-SAP also allows you to study the behavioral effects before and after treatment and subsequent elimination of vGAT expressing cells. We have demonstrated the specificity of our Anti-vGAT-SAP by cytotoxicity assays (Fig. 1).
Fig. 1: Cells were plated at 1000 cells/90 µl/well in a 96 well plate and incubated overnight. Anti-vGAT-SAP was added in 10 µl volumes and the plates were incubated for 72 hours. The plates were developed with SRB and read at 564 nm in a plate reader. Data analysis was done by PRISM (GraphPad, San Diego).
In addition to the targeted toxin, we are offering other vGAT products for your ELISA, flow cytometry and immunoblotting needs. We currently offer rabbit polyclonal vGAT antibody serum (AB-N44), affinity-purified antibody (AB-N44AP), biotinylated antibody (BT-N44AP), and Alexa-488 conjugated antibody (FL-N44AP). Keep an eye out for our transfected vGAT cell line, coming soon!