This Mac-1 mouse/human-specific antibody is from the well-characterized M1/70 (M170) clone, and was created by immunization from mouse macrophages, but reacts well with the human epitope also. It does not recognize the rat form. The antibody is also available biotin-labeled (Cat. #BT-N05) and Alexa488-labeled (Cat. #FL- N05). CD11b is an alpha subunit of Mac-1, also known as CR3. CD11b is the receptor for the C3bi fragment of complement. This receptor is involved in bacterial phagocytosis. A reduction in neutrophil CD11b expression after severe traumatic injury correlates with increased septic complications. CD11b is a component of integrins, important for adhesion of neutrophils to surfaces. Mac-1 exists as a chemoattractant activation-dependent molecule that undergoes a conformational change upon stimulation. Expression of new epitopes on Mac-1 can be detected after activation by specific reporter monoclonal antibodies. Until stimulation occurs, Mac-1 remains in a resting, non-adhesive state. Activation of Mac-1 may play a role during neutrophil recruitment to the inflamed site.
This antibody recognizes human and mouse Mac-1 (CD11b). The hybridoma was formed by the fusion of mouse myeloma NS1 cells with spleen cells from rats immunized with B10 mouse spleen cells enriched for T-lymphocytes. The antibody is routinely tested by flow cytometry.
Applications include immunoprecipitation, flow cytometry, cytotoxicity assay, binding assay and targeting.
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