Mac-1 (CD11b) Rat Monoclonal [AB-N05]

$363.00

SKU: AB-N05 Category: Quantity: 50 ug | Antibody Type: Monoclonal | Host: rat | Reactivity: human, mouse | Conjugate: unconjugated | Usage: binding assay, cytotoxicity assay, flow cytometry, immunoprecipitation |

CD11b is an alpha subunit of Mac-1, also known as CR3. CD11b is the receptor for the C3bi fragment of complement. This receptor is involved in bacterial phagocytosis. A reduction in neutrophil CD11b expression after severe traumatic injury correlates with increased septic complications. CD11b is a component of integrins, important for adhesion of neutrophils to surfaces. Mac-1 exists as a chemoattractant activation-dependent molecule that undergoes a conformational change upon stimulation. Expression of new epitopes on Mac-1 can be detected after activation by specific reporter monoclonal antibodies. Until stimulation occurs, Mac-1 remains in a resting, non-adhesive state. Activation of Mac-1 may play a role during neutrophil recruitment to the inflamed site.

This antibody recognizes human and mouse Mac-1 (CD11b). The hybridoma was formed by the fusion of mouse myeloma NS1 cells with spleen cells from rats immunized with B10 mouse spleen cells enriched for T-lymphocytes. The antibody is routinely tested by flow cytometry.

Applications include immunoprecipitation, flow cytometry, cytotoxicity assay, binding assay and targeting.

keywords: Mac-1, CD11b, C3bi receptor, microglia, macrophages, CR3, Anti-Mac-1, Anti-CD11b, Anti-CR3, brain, neuroscience

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Monoclonal xenogenetic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens.

Springer T, Galfre G, Secher DS, Milstein C (1978) Monoclonal xenogenetic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol 8:539-551. doi: 10.1002/eji.1830080802 PMID: 81133

Usage: cytotoxicity assay (ED50=16 pM), and binding assay (5 and 50 µl of culture supernatant)

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Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1.

Sanchez-Madrid F, Simon P, Thompson S, Springer TA (1983) Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med 158:586-602. doi: 10.1084/jem.158.2.586 PMID: 6193226

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Monoclonal antibody analysis of complex biological systems. Combination of cell hybridization and immunoadsorbents in a novel cascade procedure and its application to the macrophage cell surface.

Springer TA (1981) Monoclonal antibody analysis of complex biological systems. Combination of cell hybridization and immunoadsorbents in a novel cascade procedure and its application to the macrophage cell surface. J Biol Chem 256:3833-3839. doi: 10.1016/S0021-9258(19)69532-3 PMID: 7217058

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