targeted-toxins

Winkler J, Ramirez GA, Thal LJ, Waite JJ (2000) Nerve growth factor (NGF) augments cortical and hippocampal cholinergic functioning after p75NGF receptor-mediated deafferentation but impairs inhibitory avoidance and induces fear-related behaviors. J Neurosci 20:834-844. doi: 10.1523/JNEUROSCI.20-02-00834.2000

Usage: 192-SAP (Cat. #IT-01) 1.0 or 2.7 µg in 10 µl, intracerebroventricular

Related Products: 192-IgG-SAP (Cat. #IT-01)

Friedrich MJ (2000) Loss of nerve: a molecular approach to better treatment of chronic pain. JAMA 283(2):187-188. doi: 10.1001/jama.283.2.187

Summary: The use of SP-SAP (Cat. #IT-07) as a promising new method for chronic pain relief is discussed in this review article. Chronic pain has classically been treated in ways that frequently have adverse effects on the patient’s quality of life. Friedrich touches on recently developed toxins that are useful in techniques of molecular neurosurgery. These techniques allow the dissection of pain pathways in the brain and spinal cord which will provide not only a greater understanding of these pathways but also potential therapies for chronic pain and other pain conditions.

Related Products: SP-SAP (Cat. #IT-07)

Bassant MH, Poindessous-Jazat F, Schmidt BH (2000) Sustained effect of metrifonate on cerebral glucose metabolism after immunolesion of basal forebrain cholinergic neurons in rats. Eur J Pharmacol 387:151-162. doi: 10.1016/s0014-2999(99)00742-6

Usage: 192-SAP (Cat. #IT-01) 134 ng in 0.2 μl, basal forebrain

Related Products: 192-IgG-SAP (Cat. #IT-01)

Sorger D, Schliebs R, Kampfer I, Rossner S, Heinicke J, Dannenberg C, Georgi P (2000) In vivo [125I]-iodobenzovesamicol binding reflects cortical cholinergic deficiency induced by specific immunolesion of rat basal forebrain cholinergic system. Nucl Med Biol 27:23-31. doi: 10.1016/s0969-8051(99)00087-6

Usage: 192-SAP (Cat. #IT-01), 2 µg into each lateral ventricle

Related Products: 192-IgG-SAP (Cat. #IT-01)

Lehmann O, Jeltsch H, Lehnardt O, Pain L, Lazarus C, Cassel JC (2000) Combined lesions of cholinergic and serotonergic neurons in the rat brain using 192 IgG-Saporin and 5,7-dihydroxytryptamine: neurochemical and behavioural characterization. Eur J Neurosci 12:67-79. doi: 10.1046/j.1460-9568.2000.00881.x

Summary: Lesioning of septohippocampal pathways has often been used as a model for Alzheimer’s disease because these lesions alter cognitive capabilities such as spatial memory. Recent work in the behavioral neurosciences has shown that other neurotransmitter systems such as GABAergic, noradrenergic, and serotonergic systems also play a role in learning and memory. Lehmann et al. combined the effects of the cholinergic immunotoxin 192-SAP (Cat. #IT-01) and the serotonergic toxin 5,7-dihydroxytryptamine to examine interactions between these two pathways. The effects of lesioning these two pathways in concert indicate that they both play roles in cognitive functions related to working memory. [192-SAP 2 µg/lateral ventricle]

Related Products: 192-IgG-SAP (Cat. #IT-01)

Sarter M, Bruno JP (2000) Cortical cholinergic inputs mediating arousal, attentional processing and dreaming: Differential afferent regulation of the basal forebrain by telencephalic and brainstem afferents. Neuroscience 95(4):933-952. doi: 10.1016/s0306-4522(99)00487-x

Objective: To examine the cortical cholinergic inputs mediating arousal, specific attentional functions and rapid eye movement sleep-associated dreaming.

Summary: The incorporation of persistent changes in cortical information processing as a result of paired stimulation of cholinergic and sensory inputs into current theories about the cognitive functions of cortical ACh awaits more information, particularly with reference to the temporal dynamic properties of the effects of endogenously released ACh on cortical information processing.

Related Products: 192-IgG-SAP (Cat. #IT-01)

See Also:

• McGaughy J et al. Behavioral vigilance following infusions of 192 IgG-saporin into the basal forebrain: selectivity of the behavioral impairment and relation to cortical AChE-positive fiber density. Behav Neurosci 110:247-265, 1996.
• McGaughy J et al. Sustained attention performance in rats with intracortical infusions of 192 IgG-saporin-induced cortical cholinergic deafferentation: effects of physostigmine and FG 7142. Behav Neurosci 112(6):1519-1525, 1998.

Wiley RG, Lappi DA (1999) Targeting neurokinin-1 receptor-expressing neurons with [Sar9, Met(O2)11] substance P-saporin. Neurosci Lett 277(1):1-4. doi: 10.1016/s0304-3940(99)00846-0

Related Products: SSP-SAP (Cat. #IT-11)

Wrenn C, Lappi DA, Wiley RG (1999) Threshold relationship between lesion extent of the cholinergic basal forebrain in the rat and working memory impairment in the radial maze. Brain Res 847(2):284-298. doi: 10.1016/s0006-8993(99)02099-5

Related Products: 192-IgG-SAP (Cat. #IT-01)

Nichols ML, Allen BJ, Rogers SD, Ghilardi JR, Honore P, Luger NM, Finke MP, Li J, Lappi DA, Simone DA, Mantyh PW (1999) Transmission of chronic nociception by spinal neurons expressing the substance P receptor. Science 286:1558-1561. doi: 10.1126/science.286.5444.1558

Related Products: SP-SAP (Cat. #IT-07)

Kwok KHH, Law KB, Wong RNS, Yung KKL (1999) Immunolesioning of nerve growth factor p75 receptor-containing neurons in the rat brain by a novel immunotoxin: anti-p75-anti-mouse IgG-trichosanthin conjugates. Brain Res 846:154-163. doi: 10.1016/s0006-8993(99)01999-x

Related Products: 192-IgG-SAP (Cat. #IT-01)