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Model for aging in the basal forebrain cholinergic system.
Gu Z, Wortwein G, Yu J, Perez-Polo JR (2000) Model for aging in the basal forebrain cholinergic system. Antiox Redox Signal 2(3):437-447. doi: 10.1089/15230860050192215
Summary: A wide range of evidence indicates that cholinergic neurons play a role in memory and learning. Loss of these neurons is seen both in aged subjects and Alzheimer’s Disease patients. The authors discuss the use of 192-Saporin (Cat. #IT-01) to model this phenomenon. Many lesioning methods have been developed, including fimbria-fornix transections, mechanical lesions with radiofrequency or electrolysis, and intracerebral injections of excitotoxins. Information obtained through these methods suffers because non-cholinergic neurons are depleted as well as the desired cholinergic neurons. 192-Saporin provides a solution by specifically targeting and eliminating cholinergic neurons expressing p75 in the basal forebrain, closely mimicking a key component of aging.
Related Products: 192-IgG-SAP (Cat. #IT-01)
Featured Article: CBF lesioning in rabbits
Beach T (2000) Featured Article: CBF lesioning in rabbits. Targeting Trends 1(1)
Related Products: ME20.4-SAP (Cat. #IT-15)
Read the featured article in Targeting Trends.
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Entering through the doors of perception: characterization of a highly selective Substance P receptor-targeted toxin.
Lappi DA, Wiley RG (2000) Entering through the doors of perception: characterization of a highly selective Substance P receptor-targeted toxin. Neuropeptides 34(5):323-328. doi: 10.1054/npep.2000.0827
Related Products: Dermorphin-SAP / MOR-SAP (Cat. #IT-12)
Increased susceptibility to generalized seizures after immunolesions of the basal forebrain cholinergic neurons in rats.
Silveira DC, Holmes GL, Schachter SC, Geula C, Schomer DL (2000) Increased susceptibility to generalized seizures after immunolesions of the basal forebrain cholinergic neurons in rats. Brain Res 878:223-227. doi: 10.1016/s0006-8993(00)02703-7
Usage: 192-SAP (Cat. #IT-01), 4 µg intracerebroventricular injection
Related Products: 192-IgG-SAP (Cat. #IT-01)
Brainstem noradrenergic control of nociception is abnormal in the spontaneously hypertensive rat.
Taylor BK, Roderick RE, Basbaum AI (2000) Brainstem noradrenergic control of nociception is abnormal in the spontaneously hypertensive rat. Neurosci Lett 291:139-142. doi: 10.1016/s0304-3940(00)01389-6
Usage: anti-DBH-SAP (Cat. #IT-03), 5 µg
Related Products: Anti-DBH-SAP (Cat. #IT-03)
Neuropeptide-toxin conjugates in pain research and treatment. (Review)
Wiley RG (2000) Neuropeptide-toxin conjugates in pain research and treatment. (Review). Reg Anesth Pain Med 25(5):546-548. doi: 10.1053/rapm.2000.8457
Summary: Several lines of evidence indicate dorsal horn neurons that respond to substance P (SP) play a role in nociception. Wiley discusses the attributes of SP-SAP (Cat. #IT-07), a targeted toxin that eliminates cells expressing the neurokinin-1 receptor. Animals treated with this material using a lumbar intrathecal injection show a decrease in both hyperalgesia and allodynia in several pain models. The success of SP-SAP indicates that other neuropeptides, hormones, and growth factors would be useful as targeted toxins.
Related Products: SP-SAP (Cat. #IT-07)
Sympathetic reflexes after depletion of bulbospinal catecholaminergic neurons with anti-DBH-saporin.
Schreihofer AM, Guyenet PG (2000) Sympathetic reflexes after depletion of bulbospinal catecholaminergic neurons with anti-DBH-saporin. Am J Physiol Regul Integr Comp Physiol 279:R729-R742. doi: 10.1152/ajpregu.2000.279.2.R729
Related Products: Anti-DBH-SAP (Cat. #IT-03)
Effects of cholinergic depletion on neural activity in different laminae of the rat barrel cortex.
Herron P, Schweitzer JB (2000) Effects of cholinergic depletion on neural activity in different laminae of the rat barrel cortex. Brain Res 872:71-76. doi: 10.1016/s0006-8993(00)02454-9
Summary: 192-SAP (Cat. #IT-01) 8.0 µg/300 g body weight, nucleus basalis of Meynert
Related Products: 192-IgG-SAP (Cat. #IT-01)
Antibody for human p75 LNTR identifies cholinergic basal forebrain of non-primate species.
Tremere LA, Pinaud R, Grosche J, Hartig W, Rasmusson DD (2000) Antibody for human p75 LNTR identifies cholinergic basal forebrain of non-primate species. NeuroReport 11(10):2177-2183. doi: 10.1097/00001756-200007140-00023 PMID: 10923666
Summary: 192-SAP (Cat. #IT-01) is a highly successful reagent for eliminating cholinergic neurons in rats. Because the targeting antibody only recognizes rat p75, it is unable to be used in other species. Tremere et al. have stained basal forebrain sections with ME20.4, a monoclonal antibody to human p75 (Cat. #AB-N07) and found excellent cross-reactivity in dog, raccoon, cat, pig and rabbit. The authors state that an ME20.4-saporin conjugate could be used to produce cholinergic basal forebrain lesions in several species.
Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07), 192-IgG-SAP (Cat. #IT-01)
Sustained visual attention performance-associated prefrontal neuronal activity: evidence for cholinergic modulation.
Gill TM, Sarter M, Givens B (2000) Sustained visual attention performance-associated prefrontal neuronal activity: evidence for cholinergic modulation. J Neurosci 20:4745-4757. doi: 10.1523/JNEUROSCI.20-12-04745.2000
Summary: Preliminary evidence suggests that demands on attention levels are associated with changes in levels of cortical acetylcholine. Gill et al. used .05 µg 192-Saporin (Cat. #IT-01) by intracortical infusion to demonstrate the role cholinergic neurons play in the ability of rats to pay attention. The researchers monitored medial prefrontal cortex (MPC) activity in the rat brain before and after elimination of cholinergic neurons with 192-Saporin. The results suggest that the cholinergic inputs to the MPC influence the increases of neuronal activity associated with paying attention.
Related Products: 192-IgG-SAP (Cat. #IT-01)