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Ectopic noradrenergic hyperinnervation does not functionally compensate for neonatal forebrain acetylcholine lesion.
Pappas BA, Nguyen T, Brownlee B, Tanasoiu D, Fortin T, Sherren N (2000) Ectopic noradrenergic hyperinnervation does not functionally compensate for neonatal forebrain acetylcholine lesion. Brain Res 867:90-99. doi: 10.1016/s0006-8993(00)02263-0
Summary: Removal of cholinergic forebrain neurons in the neonatal rat causes an ingrowth of hippocampal material to the affected area. The behavioral effect of this treatment increases working, but not reference memory errors on the radial arm maze. Pappas et al. used 300 ng 192-Saporin (Cat. #IT-01) by i.c.v. injection to lesion the forebrain of 1- to 3-day old rats coupled with a 6-OHDA lesion to remove hippocampal ingrowths in order to investigate whether these neurons can compensate for cholinergic function in memory. Their results indicate noradrenergic neurons from the hippocampus do not functionally compensate for loss of CBF neurons even though losses of these neurons did not drastically affect the behavior of these animals.
Related Products: 192-IgG-SAP (Cat. #IT-01)
Basal forebrain neurons suppress amygdala kindling via cortical but not hippocampal cholinergic projections in rats.
Ferencz I, Leanza G, Nanobashvili A, Kokaia M, Lindvall O (2000) Basal forebrain neurons suppress amygdala kindling via cortical but not hippocampal cholinergic projections in rats. Eur J Neurosci 12:2107-2116. doi: 10.1046/j.1460-9568.2000.00077.x
Summary: Cholinergic mechanisms have been implicated in human epilepsy, possibly in the role of seizure suppression. Ferencz et al. used 2.5 µg 192-Saporin (Cat. #IT-01) by i.c.v. injection to investigate the effect of eliminating cholinergic projections to the hippocampal formation and cerebral cortex on the induction of epilepsy through electrical stimulation of the rat brain. The researchers used the specificity of 192-Saporin to determine that the loss of specific projections to the amygdala accelerates development of seizures. The hippocampus does not influence this process.
Related Products: 192-IgG-SAP (Cat. #IT-01)
Preferential destruction of cerebellar Purkinje cells by OX7-saporin.
Angner RT, Kelly RM, Wiley RG, Walsh TJ, Reuhl KR (2000) Preferential destruction of cerebellar Purkinje cells by OX7-saporin. Neurotoxicol 21:395-403.
Summary: Purkinje cells function as inhibitors and are the sole output of the cerebellar cortex. Angner et al. eliminate these cells in rats with 1-2 µg OX7-SAP (Cat. #IT-02), an immunotoxin that binds the Thy 1.1 antigen. The treated rats show effects of loss of inhibitory control, including a time-dependent increase in motor activity and decreased motor coordination.
Related Products: OX7-SAP (Cat. #IT-02)
[Non-cholinergic projections from basal forebrain to medial limbic cortex of rat].
Murakami K, Yokofujita J, Kuroda M (2000) [Non-cholinergic projections from basal forebrain to medial limbic cortex of rat]. Kaibogaku Zasshi 75:285-297.
Related Products: 192-IgG-SAP (Cat. #IT-01)
Synergistic effects of unilateral immunolesions of the cholinergic projections from the basal forebrain and contralateral ablations of the inferotemporal cortex and hippocampus in monkeys.
Barefoot HC, Baker HF, Ridley RM (2000) Synergistic effects of unilateral immunolesions of the cholinergic projections from the basal forebrain and contralateral ablations of the inferotemporal cortex and hippocampus in monkeys. Neuroscience 98(2):243-251. doi: 10.1016/s0306-4522(00)00131-7
Related Products: ME20.4-SAP (Cat. #IT-15)
NGF-mediated alteration of NF-κB binding activity after partial immunolesions to rat cholinergic basal forebrain neurons.
Gu Z, Toliver-Kinsky T, Glasgow J, Werrbach-Perez K, Perez-Polo JR (2000) NGF-mediated alteration of NF-κB binding activity after partial immunolesions to rat cholinergic basal forebrain neurons. Int J Dev Neurosci 18:455-468. doi: 10.1016/s0736-5748(00)00004-6
Summary: After injecting 1.3 µg 192-Saporin (Cat. #IT-01) into the lateral ventricle of rat brain, followed by infusion of NGF antibody, Gu et al. report changes in the activity of the transcription factor NF-B. Aged rodent brains show an increase in NF-kappaB activity. This model creates a tool to investigate decreased cholinergic function that is often associated with memory loss and cognitive deficits in the elderly and particularly in patients with Alzheimer’s disease.
Related Products: 192-IgG-SAP (Cat. #IT-01)
Immunolocalization of the cocaine- and antidepressant-sensitive 1-norepinephrine transporter.
Schroeter S, Apparsundaram S, Wiley RG, Miner LH, Sesack SR, Blakely RD (2000) Immunolocalization of the cocaine- and antidepressant-sensitive 1-norepinephrine transporter. J Comp Neurol 420:211-232. doi: 10.1002/(SICI)1096-9861(20000501)420:2<211::AID-CNE5>3.0.CO;2-3
Summary: Norepinephrine transporters are involved in the response to multiple antidepressants and psychostimulants, but the expression of these proteins has not yet been characterized in the central nervous system. Schroeter et al. used an antibody to a cytoplasmic epitope of norepinephrine transporters to map the transporters to noradrenergic neuronal somata, axons, and dendrites. To verify the specificity of the antibody the researchers injected 10 µg of anti-DBH-SAP (Cat. #IT-03) in the left lateral ventricle of rats to destroy the noradrenergic neurons, confirming the specificity of the norepinephrine transporter antibody. Treatment with anti-DBH-SAP completely removed norepinephrine transporter immunoreactivity.
Related Products: Anti-DBH-SAP (Cat. #IT-03)
Cortical cholinergic denervation elicits vascular A beta deposition.
Roher AE, Kuo YM, Potter PE, Emmerling MR, Durham RA, Walker DG, Sue LI, Honer WG, Beach TG (2000) Cortical cholinergic denervation elicits vascular A beta deposition. Ann NY Acad Sci 903:366-373. doi: 10.1111/j.1749-6632.2000.tb06388.x
Related Products: ME20.4-SAP (Cat. #IT-15)
Impaired acquisition of a Morris water maze task following selective destruction of cerebellar Purkinje cells with OX7-saporin.
Gandhi CC, Kelly RM, Wiley RG, Walsh TJ (2000) Impaired acquisition of a Morris water maze task following selective destruction of cerebellar Purkinje cells with OX7-saporin. Behav Brain Res 109:37-47. doi: 10.1016/s0166-4328(99)00160-6
Summary: The cerebellum has been associated with the control of motor activity and voluntary movements. Recent data have shown the cerebellum may also play a role in “higher order” processes such as learning, language, and cognition. Using 2 µg OX7-SAP (Cat. #IT-02) by i.c.v. injection, Gandhi et al. selectively eliminated Purkinje cells in rat cerebellum in order to examine the ability of treated animals to complete a water maze task. Elimination of these cells significantly impaired the ability of the rats to complete the task, suggesting the cerebellum is involved in learning.
Related Products: OX7-SAP (Cat. #IT-02)
Cholinergic deafferentation of the rabbit cortex: a new animal model of Abeta deposition.
Beach TG, Potter PE, Kuo YM, Emmerling MR, Durham RA, Webster SD, Walker DG, Sue LI, Scott S, Layne KJ, Roher AE (2000) Cholinergic deafferentation of the rabbit cortex: a new animal model of Abeta deposition. Neurosci Lett 283:9-12. doi: 10.1016/s0304-3940(00)00916-2
Related Products: ME20.4-SAP (Cat. #IT-15)