targeted-toxins

Dommergues MA, Plaisant F, Verney C, Gressens P (2003) Early microglial activation following neonatal excitotoxic brain damage in mice: a potential target for neuroprotection. Neuroscience 121(3):619-628. doi: 10.1016/s0306-4522(03)00558-x

Summary: Brain lesions that mimic damage from cerebral palsy in mice are characterized by microglial activation within 24 hours of insult. Using intraperitoneal injections of Mac-1-SAP (90 µg/kg, Cat. #IT-06), a reduction in the density of resident microglial and blood-derived monocytes was obtained.

Related Products: Mac-1-SAP mouse/human (Cat. #IT-06)

Leung LS, Shen B, Rajakumar N, Ma J (2003) Cholinergic activity enhances hippocampal long-term potentiation in CA1 during walking in rats. J Neurosci 23(28):9297-9304. doi: 10.1523/JNEUROSCI.23-28-09297.2003

Summary: To investigate the role of the cholinergic system in long term potentiation (LTP) the authors lesioned the left and right medial septum of rats with 0.14 µg of 192-Saporin (Cat. #IT-01). LTP induced in lesioned walking animals is less robust than in control animals.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Kanold PO (2003) Featured Article: Subplate neurons and functional maturation of thalamocortical synapses. Targeting Trends 4(4)

Related Products: ME20.4-SAP (Cat. #IT-15)

Read the featured article in Targeting Trends.

See Also:

• Kanold PO et al. Role of subplate neurons in functional maturation of visual cortical columns. Science 301(5632):521-525, 2003.

Porreca F, Hruby V, Lai J (2003) Featured Article: CCK-SAP in binding studies. Targeting Trends 4(4)

Related Products: CCK-SAP (Cat. #IT-31)

Read the featured article in Targeting Trends.

Wiley RG, Harrison MB, Levey A, Lappi DA (2003) Destruction of midbrain dopaminergic neurons by using an immunotoxin to the dopamine transporter. Cell Mol Neurobiol 23:839-850. doi: 10.1023/a:1025065306264

Summary: The authors demonstrate the effective and specific removal of neurons expressing the dopamine transporter in the substantia nigra pars compacta and the ventral tegmental area with anti-DAT-SAP (Cat. #IT-25). A 21-µg icv injection produced a highly significant loss of midbrain dopaminergic neurons, creating a useful model for Parkinson’s disease.

Related Products: Anti-DAT-SAP (Cat. #IT-25)

Dudkin KN, Chueva IV, Makarov FN (2003) [Interaction between sensory and cognitive processes in visual recognition: the role of the associative areas of the cerebral cortex] Russian. Ross Fiziol Zh Im I M Sechenova 89(10):1226-1239.

Summary: The authors used ME20.4-SAP(Cat. #IT-15).

Related Products: ME20.4-SAP (Cat. #IT-15)

Madden CJ, Sved AF (2003) Rostral ventrolateral medulla C1 neurons and cardiovascular regulation. Cell Mol Neurobiol 23(4-5):739-749. doi: 10.1023/a:1025000919468

Summary: The authors review the use of anti-DBH-SAP (Cat. #IT-03) to study the role of C1 neurons within the rostral ventromedial medulla in cardiovascular regulation. This immunotoxin specifically removes C1 neurons containing dopamine beta-hydroxylase.

Related Products: Anti-DBH-SAP (Cat. #IT-03)

I’Anson H, Sundling LA, Roland SM, Ritter S (2003) Immunotoxic destruction of distinct catecholaminergic neuron populations disrupts the reproductive response to glucoprivation in female rats. Endocrinology 144(10):4325-4331. doi: 10.1210/en.2003-0258

Summary: The authors hypothesized that hindbrain catcholamine neurons suppressed estrous cycles during chronic glucoprivation as an extension of their role in glucoprivic feeding. 42-ng bilateral injections of anti-DBH-SAP (Cat. #IT-03) were made into the paraventricular nucleus of female rats. Lesioned rats demonstrated inhibition of reproductive function during chronic glucose deficit, but not when a normal amount of glucose was available.

Related Products: Anti-DBH-SAP (Cat. #IT-03), Saporin (Cat. #PR-01)

Tomaszewicz M, Rossner S, Schliebs R, Cwikowska J, Szutowicz A (2003) Changes in cortical acetyl-CoA metabolism after selective basal forebrain cholinergic degeneration by 192IgG-saporin. J Neurochem 87(2):318-324. doi: 10.1046/j.1471-4159.2003.01983.x

Objective: To investigate whether cortical cholinergic input affects acetyl-CoA metabolism in cholinoceptive cortical target regions.

Summary: Alzheimer’s disease subjects often show deficits in cerebral glucose metabolism. The data show evidence of differential distribution of acetyl-CoA in subcellular compartments of cholinergic and non-cholinergic nerve terminals.

Usage: Rats received 4 µg 192-IgG-SAP (Cat. #IT-01) into the left lateral ventricle.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Gadd CA, Murtra P, De Felipe C, Hunt SP (2003) Neurokinin-1 receptor-expressing neurons in the amygdala modulate morphine reward and anxiety behaviors in the mouse. J Neurosci 23(23):8271-8280. doi: 10.1523/JNEUROSCI.23-23-08271.2003

Summary: Mice lacking the neurokinin-1 (NK-1) receptor are insensitive to opiates in models of drug abuse. To assess what areas of the brain may be involved in this process, the authors used 1.0-µl injections of 1.0 µM SP-SAP (Cat. #IT-07) to eliminate NK-1 receptor-positive neurons in the nucleus accumbens, dorsomedial caudate putamen or amygdala of mice. Only mice with amygdala lesions displayed behavior comparable to NK-1 receptor knockout mice—increase in anxiety-like behavior, reduction in stimulant effect of morphine. These data suggest that the amygdala plays an important role in anxiety behaviors and the response to opiates.

Related Products: SP-SAP (Cat. #IT-07)