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Postnatal development and functional adaptations of the melanopsin photoreceptive system in the albino mouse retina.
Gonzalez-Menendez I, Contreras F, Cernuda-Cernuda R, Provencio I, Garcia-Fernandez JM (2010) Postnatal development and functional adaptations of the melanopsin photoreceptive system in the albino mouse retina. Invest Ophthalmol Vis Sci 51(9):4840-4847. doi: 10.1167/iovs.10-5253 PMID: 20435589
Summary: Melanopsin-expressing intrinsically photosensitive retinal ganglion cells (ipRGCs) adjust the circadian pacemaker of mammals by detecting light. The authors tracked the development of ipRGCs in postnatal mice under varying light conditions. Immunohistochemistry for these experiments was done using an anti-mouse melanopsin polyclonal antibody (Cat. #AB-N38). Alteration of the standard light/dark cycle clearly affected the development of ipRGCs.
Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)
Derivation of neural crest cells from human pluripotent stem cells
Lee G, Chambers SM, Tomishima MJ, Studer L (2010) Derivation of neural crest cells from human pluripotent stem cells. Nat Protoc 5(4):688-701. doi: 10.1038/nprot.2010.35 PMID: 20360764
Summary: Protocols are presented for the purification and propagation of hPSC-NC cells using flow cytometry and defined in vitro culture conditions. This protocol has been validated in multiple independent hESC and hiPSC lines. The average time required for generating purified hPSC-NC precursors using this protocol is 2–5 weeks.
Usage: Neural crest cells (1:200).
Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)
A novel approach to preventing the hemolysis of paroxysmal nocturnal hemoglobinuria: both complement-mediated cytolysis and C3 deposition are blocked by a monoclonal antibody specific for the alternative pathway of complement
Lindorfer MA, Pawluczkowycz AW, Peek EM, Hickman K, Taylor RP, Parker CJ (2010) A novel approach to preventing the hemolysis of paroxysmal nocturnal hemoglobinuria: both complement-mediated cytolysis and C3 deposition are blocked by a monoclonal antibody specific for the alternative pathway of complement. Blood 115(11):2283-2291. doi: 10.1182/blood-2009-09-244285 PMID: 20068220
Related Products: Antibody to Complement C3 (7C12) (Cat. #AB-V84)
Anti-Melanopsin Protocol
Q: We’re interested in trying out your melanopsin antibody (Cat. #AB-N38) using immunohistochemistry in mouse retina. Do you have a recommended protocol?
A: This protocol has been utilized successfully with anti-melanopsin.
Related: Anti-Melanopsin (Cat. #AB-N38), Anti-Melanopsin, affinity-purified (Cat. #AB-N39)
Serotonin transport and metabolism in the mammary gland modulates secretory activation and involution.
Marshall AM, Nommsen-Rivers LA, Hernandez LL, Dewey KG, Chantry CJ, Gregerson KA, Horseman ND (2010) Serotonin transport and metabolism in the mammary gland modulates secretory activation and involution. J Clin Endocrinol Metab 95(2):837-846. doi: 10.1210/jc.2009-1575 PMID: 19965920
Summary: Serotonin is known to be a local regulator of lactation homeostasis. This work examined the roles of the serotonin reuptake transporter (SERT) and monoamine oxidase in this system. Immunohistochemical and immunocytochemical staining was done on human primary mammary epithelial cells and mouse tissue with an anti-SERT antibody (Cat. #AB-N09). Additional data included epidemiological studies and selective serotonin reuptake inhibitor treatment of mice. The results suggest that women taking SSRI inhibitor medications were more likely to experience delayed secretory activation.
Related Products: Antibody to Serotonin Transporter (SERT, Cat. #AB-N09)
Proteomic analysis uncovers novel actions of the neurosecretory protein VGF in nociceptive processing.
Riedl MS, Braun PD, Kitto KF, Roiko SA, Anderson LB, Honda CN, Fairbanks CA, Vulchanova L (2009) Proteomic analysis uncovers novel actions of the neurosecretory protein VGF in nociceptive processing. J Neurosci 29:13377-13388. doi: 10.1523/JNEUROSCI.1127-09.2009 PMID: 19846725
Summary: Peripheral tissue injury can alter protein expression in sensory neurons, which may contribute to abnormal nociceptive processing. The authors used cultured dorsal root ganglion neurons as a model for axotomized neurons to examine early changes in protein expression after nerve injury. Several different parameters were measured, including immunohistochemistry using anti-TrkA (Cat. #AB-N03). The data show an increased level of a putative neuropeptide precursor, VGF, as a result of nerve injury.
Related Products: trkA Rabbit Polyclonal (Cat. #AB-N03)
cGMP-dependent protein kinase Ialpha associates with the antidepressant-sensitive serotonin transporter and dictates rapid modulation of serotonin uptake.
Steiner J, Carneiro A, Wright J, Matthies H, Prasad H, Nicki C, Dostmann W, Buchanan C, Corbin J, Francis S, Blakely R (2009) cGMP-dependent protein kinase Ialpha associates with the antidepressant-sensitive serotonin transporter and dictates rapid modulation of serotonin uptake. Mol Brain 2:26. doi: 10.1186/1756-6606-2-26 PMID: 19656393
Summary: The neurotransmitter serotonin fulfills an important modulatory role in a wide range of brain functions including mood, appetite, sexual behavior, and reward. Serotonin transporters (SERT) are involved in the inactivation of synaptic serotonin, as well as serotonin recycling, which is critical to the maintenance of neuronal serotonin stores. In this work the authors examined how neuronal A3 adenosine receptor activation can enhance presynaptic serotonin transport in vitro as well as SERT-mediated clearance in vivo. The in vitro experiments included immunohistochemistry with anti-SERT (Cat. #AB-N40) on RN46A cells at a 1:500 dilution.
Related Products: SERT Mouse Monoclonal (Cat. #AB-N40)
Basal cells as stem cells of the mouse trachea and human airway epithelium
Rock JR, Onaitis MW, Rawlins EL, Lu Y, Clark CP, Xue Y, Randell SH, Hogan BL (2009) Basal cells as stem cells of the mouse trachea and human airway epithelium. Proc Natl Acad Sci U S A 106(31):12771-12775. doi: 10.1073/pnas.0906850106 PMID: 19625615
Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)
Antigen for HRP-labeled Antibody to p53
Q: Regarding your HRP-labeled Antibody to p53 (Cat. #AB-236), your data sheet states the antigen was 15-40 a.a. Does this a.a. count from N-terminus?
A: There was an error on the data sheet which has since been corrected. The AB-236 immunogen is a KLH-conjugated peptide corresponding to amino acids 6-45. The numbering does start from the N-terminus.
Somatostatin Antibodies
Q: Could you please tell me if the Somatostatin 14 antibody (Cat. #AB-04) will also pick up the Somatostatin 28 residue?
A: Yes, it will, because they share the sequence of SS14. However, the Somatostatin-28 antibody (Cat. #AB-05) will not see Somatostatin-14.
Related: Anti-Somatostatin-14 (Cat. #AB-04), Anti-Somatostatin-28 (Cat. #AB-05)