antibodies

Koch H, Zanella S, Elsen GE, Smith L, Doi A, Garcia AJr, Wei AD, Xun R, Kirsch S, Gomez CM, Hevner RF, Ramirez JM (2013) Stable respiratory activity requires both P/Q-type and N-type voltage-gated calcium channels. J Neurosci 33(8):3633-3645. doi: 10.1523/JNEUROSCI.6390-11.2013 PMID: 23426690

Summary: Pharmacological experiments have suggested that sighs and normal respiration are highly dependent on calcium currents carried by P/Q channels. Using transgenic mice missing the Cav2.1 pore-forming α1A subunit the authors demonstrate that loss of P/Q-type calcium channels results in compromised breathing, sighing, neuromodulation, and leads to early death. A neurokinin-1 receptor antibody (Cat. #AB-N33AP) at a 1:500 dilution was used for immunohistochemistry in this work.

Related Products: NK-1 Receptor Rabbit Polyclonal, affinity-purified (Cat. #AB-N33AP)

Shia WW, Bailey RC (2013) Single domain antibodies for the detection of ricin using silicon photonic microring resonator arrays. Anal Chem 85(2):805-810. doi: 10.1021/ac3030416 PMID: 23268548

Summary: A major hurdle to clear in the fight against bioterrorism is the ability to identify various biowarfare agents. One of the more difficult substances to identify is ricin. This work describes the use of single domain antibodies to identify ricin in a microring resonator array assay. Saporin (Cat. #PR-01) along with affinity purified chicken anti-saporin (Cat. #AB-17AP) were used as controls when constructing the assay. The results demonstrate the feasibility of using microring resonator arrays for the detection of biowarfare agents.

Related Products: Saporin Chicken Polyclonal, affinity-purified (Cat. #AB-17AP), Saporin (Cat. #PR-01)

Menendez L, Kulik MJ, Page AT, Park SS, Lauderdale JD, Cunningham ML, Dalton S (2013) Directed differentiation of human pluripotent cells to neural crest stem cells. Nat Protoc 8(1):203-212. doi: 10.1038/nprot.2012.156 PMID: 23288320

Summary: This protocol describes in detail how to perform a highly efficient, lineage-specific differentiation of human pluripotent cells to a NCSC fate.

Usage: 0.2 ul per 10^6 cells

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Estevez ME, Fogerson PM, Ilardi MC, Borghuis BG, Chan E, Weng S, Auferkorte ON, Demb JB, Berson DM (2012) Form and function of the M4 cell, an intrinsically photosensitive retinal ganglion cell type contributing to geniculocortical vision. J Neurosci 32(39):13608-13620. doi: 10.1523/JNEUROSCI.1422-12.2012 PMID: 23015450

Summary: Intrinsically photosensitive retinal ganglion cells (ipRGCs) are cells that contain the photopigment melanopsin. In this work the authors extensively characterize the M4 ipRGCs. A melanopsin antibody (Cat. #AB-N38) at a 1:10,000 dilution was used to determine the presence of melanopsin by immunohistochemistry.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Kirillova V, Prosviryakov E (2012) Disrupted serotonergic system in patients with pulmonary hypertension may serve as novel biomarkers new therapeutic targets and to assess severity, progression and response to treatment. Cardiovasc Res 93 Suppl 1:P209. European Society of Cardiology, Munich Germany. PMID: 909090

Summary: At the Frontiers in Cardiovascular Biology meeting in London the authors presented work examining the role serotonin and serotonin transporters play in pulmonary hypertension. Anti-SERT (Cat. #AB-N09, 1:500) was used in immunohistochemistry to detect the serotonin transporter in the myocardium.  The data demonstrate that serotonin levels in the blood and serotonin transporter levels in the myocardium are both increased in patients with pulmonary hypertension.

Related Products: Antibody to Serotonin Transporter (SERT, Cat. #AB-N09)

Lacerda CM, Kisiday J, Johnson B, Orton EC (2012) Local serotonin mediates cyclic strain-induced phenotype transformation, matrix degradation, and glycosaminoglycan synthesis in cultured sheep mitral valves. Am J Physiol Heart Circ Physiol 302(10):H1983-90. doi: 10.1152/ajpheart.00987.2011 PMID: 22345569

Summary: Calcific aortic valve disease and myxomatous mitral valve disease (MMVD) are the most commonly seen degenerative heart valve diseases. Exogenous serotonin has been shown to induce valvulopathy, but current opinion regards serotoninergic and degenerative valvulopathies as unrelated. This work investigated the relationship between serotonin synthesis and strain-induced MMVD in cultured sheep mitral valve leaflets. Anti-SERT (Cat. #AB-N09) was used in immunoblotting experiments. The results demonstrate local serotonin synthesis by mitral valves, modulation of this synthesis by tensile loading, and inhibition of serotonin results in the reduction of strain-mediated protein expression.

Related Products: Antibody to Serotonin Transporter (SERT, Cat. #AB-N09)

Hu J, Huang T, Li T, Guo Z, Cheng L (2012) c-Maf is required for the development of dorsal horn laminae III/IV neurons and mechanoreceptive DRG axon projections. J Neurosci 32(16):5362-5373. doi: 10.1523/JNEUROSCI.6239-11.2012 PMID: 22514301

Summary: The molecular mechanisms responsible for development of laminae III/IV neurons are not yet well understood. In this work the authors investigated the role of c-Maf, a basic leucine-zipper transcription factor from the AP-1 superfamily. Anti-TrkA (Cat. #AB-N03: 1:100) was used for immunohistochemistry.

Related Products: trkA Rabbit Polyclonal (Cat. #AB-N03)

Sanchez-Ortiz E, Yui D, Song D, Li Y, Rubenstein JL, Reichardt LF, Parada LF (2012) TrkA gene ablation in basal forebrain results in dysfunction of the cholinergic circuitry. J Neurosci 32(12):4065-4079. doi: 10.1523/JNEUROSCI.6314-11.2012 PMID: 22442072

Summary: The authors created a conditional trkA knockout mouse line. Anti-trkA (Cat. #AB-N03) was used for immunohistochemistry (1:1000) and western blots (1:4000). The data demonstrate the importance of trkA in the establishment of basal forebrain cholinergic circuitry, and choline acetyltransferase expression.

Related Products: trkA Rabbit Polyclonal (Cat. #AB-N03)

Gibbons DD, Southerland EM, Hoover DB, Beaumont E, Armour JA, Ardell JL (2012) Neuromodulation targets intrinsic cardiac neurons to attenuate neuronally mediated atrial arrhythmias. Am J Physiol Regul Integr Comp Physiol 302(3):R357-R364. doi: 10.1152/ajpregu.00535.2011 PMID: 22088304

Summary: Cardiac arrhythmias can be generated by excessive activation of specific inputs to the intrinsic cardiac nervous system. The authors sought to determine whether subpopulations of neurons were responsible for this activation, and therefore potential therapeutic targets. A series of studies were done following electrical stimuli to the mediastinal nerves. Choline acetyltransferase levels were assessed using anti-ChAT (Cat. #AB-N34) in immunohistochemistry. The data suggest activation of certain neurons by mediastinal nerve stimulation results in atrial arrhythmias leading to atrial fibrillation.

Related Products: Choline Acetyltransferase Rabbit Polyclonal (Cat. #AB-N34)

Choi JI, Koehrn FJ, Sorkin LS (2012) Carrageenan induced phosphorylation of Akt is dependent on neurokinin-1 expressing neurons in the superficial dorsal horn. Mol Pain 8(1):4. doi: 10.1186/1744-8069-8-4 PMID: 22243518

Summary: In this work the authors administered 100 ng SSP-SAP (Cat. #IT-11) into the intrathecal space of rats (saporin, Cat. #PR-01, was used as a control). Lesioned animals displayed decreased carrageenan-induced mechanical allodynia, and carrageenan-induced phosphorylation of Akt was blocked throughout the spinal cord gray matter. Anti-NK-1 (Cat. #AB-N33AP) was used for immunohistochemistry.

Related Products: SSP-SAP (Cat. #IT-11), Saporin (Cat. #PR-01), NK-1 Receptor Rabbit Polyclonal, affinity-purified (Cat. #AB-N33AP)