antibodies

Grillo SL, Stella SL, Jr. (2018) Melanopsin retinal ganglion cells are not labeled in Thy-1YFP-16 transgenic mice. Neuroreport 29:118-122. doi: 10.1097/WNR.0000000000000918 PMID: 29251688

Objective: To determine whether mRGCs are labeled with YFP in Thy-1 YFP-16 transgenic mice. The transgenic Thy-1 YFP mouse line 16 (Thy-1 YFP-16) expresses yellow-fluorescent protein (YFP) in projection neurons, including RGCs.

Summary: The majority of mRGC somas and axons are not labeled with YFP in the transgenic Thy-1 YFP-16 mouse line; therefore, this mouse model may not suitable for research involving mRGC visual pathways.

Usage: Affinity purified rabbit polyclonal antibody raised against melanopsin (1:500) was used to label mRGCs (Retinal ganglion cells expressing melanopsin). Immunolabeled Thy-1 YFP-16 mouse retina with anti-melanopsin and found that majority (∼89%) of mRGCs are not YFP-positive despite numerous other YFP-positive RGCs in the retina.

Related Products: Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)

Liu W, Zhao Y, Zhang X, Ji J (2018) Simvastatin ameliorates cognitive impairments via inhibition of oxidative stress‑induced apoptosis of hippocampal cells through the ERK/AKT signaling pathway in a rat model of senile dementia. Mol Med Rep 17(1):1885-1892. doi: 10.3892/mmr.2017.8098 PMID: 29257256

Objective: To investigate a potential mechanism underlying simvastatin activity in hippocampal cells.

Summary: The preclinical results indicate that simvastatin is efficient in preventing memory lapse and inhibiting apoptosis of hippocampal cells via the ERK/AKT signaling pathway, which may in the future improve cognitive decline and dementia in patients.

Usage: immunohistochemistry (1:500)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Hamano S, Tomokiyo A, Hasegawa D, Yoshida S, Sugii H, Mitarai H, Fujino S, Wada N, Maeda H (2017) Extracellular matrix from periodontal ligament cells could induce the differentiation of induced pluripotent stem cells to periodontal ligament stem cell-like cells. Stem Cells Dev 27:100-111. doi: 10.1089/scd.2017.0077 PMID: 29160151

Usage: Immunofluorescence staining (1:200 dilution)

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Trifunovic S, Manojlovic-Stojanoski M, Nestorovic N, Ristic N, Sosic-Jurjevic B, Pendovski L, Milosevic V (2018) Histological and morphofunctional parameters of the hypothalamic-pituitary-adrenal system are sensitive to daidzein treatment in the adult rat. Acta Histochem 120:129-135. doi: 10.1016/j.acthis.2017.12.006 PMID: 29338944

Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)

Delwig A, Chaney S, Bertke A, Verweij J, Quirce S, Larsen D, Yang C, Buhr E, VAN Gelder R, Gallar J, Margolis T, Copenhagen D (2018) Melanopsin expression in the cornea. Vis Neurosci 35:E004. doi: 10.1017/S0952523817000359 PMID: 29905117

Objective: To determine melanopsin expression in cornea.

Summary: Found no light-evoked activation of melanopsin-expressing fibers in cornea or in cell bodies in the TG; melanopsin protein might serve other sensory functions in the cornea. One justification for this idea is that melanopsin expressed in Drosophila photoreceptors can serve as a temperature sensor.

Usage: For the primary staining, we used Anti-Melanopsin at 1:5000 for 3 days.

Related Products: Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)

Stabio ME, Sabbah S, Quattrochi LE, Ilardi MC, Fogerson PM, Leyrer ML, Kim MT, Kim I, Schiel M, Renna JM, Briggman KL, Berson DM (2018) The M5 cell: A color-opponent intrinsically photosensitive retinal ganglion cell. Neuron 97:150-163. doi: 10.1016/j.neuron.2017.11.030 PMID: 29249284

Objective: To provide a fuller description of the least understood ipRGC type, the M5 cell, and discovered a distinctive functional characteristic— chromatic opponency (ultraviolet excitatory, green inhibitory).

Summary: M5 cells send axons to the dLGN and are thus positioned to provide chromatic signals to visual cortex. These findings under- score that melanopsin’s influence extends beyond unconscious reflex functions to encompass cortical vision, perhaps including the perception of color.

Usage: M5 cells have the weakest melanopsin responses of all known ipRGC types. The intrinsic melanopsin response (~10 pA) in M5 cells is at least an order of magnitude smaller than the extrinsic, synaptically mediated response.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Fujita M, Fukuda T, Sato Y, Takasusuki T, Tanaka M (2018) Allopregnanolone suppresses mechanical allodynia and internalization of neurokinin-1 receptors at the spinal dorsal horn in a rat postoperative pain model. Korean J Pain 31:10-15. doi: 10.3344/kjp.2018.31.1.10. PMID: 29372021

Objective: To identify a new strategy for postoperative pain management, this study investigated the analgesic effects of allopregnanolone (Allo) in an incisional pain model, and its effects on the activities of the primary afferent fibers at the dorsal horn.

Summary: Systemic administration of Allopregnanolone inhibited mechanical allodynia and activities of the primary afferent fibers at the dorsal horn in a rat postoperative pain model. Allopregnanolone was proposed as a candidate for postoperative pain management.

Usage: Fluorescence immunohistochemistry: Six sections per animal were labeled with primary antiserum, rabbit anti-NK-1R (1:3000).

Related Products: NK-1 Receptor Rabbit Polyclonal, affinity-purified (Cat. #AB-N33AP)

Review

Thom G, Hatcher J, Hearn A, Paterson J, Rodrigo N, Beljean A, Gurrell I, Webster C (2018) Isolation of blood-brain barrier-crossing antibodies from a phage display library by competitive elution and their ability to penetrate the central nervous system. mAbs 10:304-314. doi: 10.1080/19420862.2017.1409320 PMID: 29182455

Summary: Fluorescence microvolume assay technology (FMAT) periprep extract or Nickel-purified scFvs from E. coli were transferred into assay plates containing 10 ml (1:1000 dilution of a 1 mg/ml stock solution) mouse anti-HIS MAb.)

Related Products: 6His Mouse Monoclonal (Cat. #AB-213)

Ullah K, Rosendahl AH, Izzi V, Bergmann U, Pihlajaniemi T, Maki JM, Myllyharju JA-Ohoo (2017) Hypoxia-inducible factor prolyl-4-hydroxylase-1 is a convergent point in the reciprocal negative regulation of NF-κB and p53 signaling pathways. Sci Rep 7:172200. doi: 10.1038/s41598-017-17376-0 PMID: 29222481

Usage: Western blot

Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)

Brick RM, Sun AX, Tuan RSA-Ohoo (2018) Neurotrophically induced mesenchymal progenitor cells derived from induced pluripotent stem cells enhance neuritogenesis via neurotrophin and cytokine production. Stem Cells Transl Med 7:45-58. doi: 10.1002/sctm.17-0108 PMID: 29215199

Objective: To examine the potential utility of an alternative, mesenchymal-like cell (MSCs) source, derived from induced pluripotent stem cells, termed induced mesenchymal progenitor cells (MiMPCs) to produce bioactive factors and support nerve regeneration.

Summary: The findings suggest MiMPCs as a renewable, candidate source of therapeutic cells and a potential alternative to MSCs for peripheral nerve repair, in view of their ability to promote nerve growth by producing many of the same growth factors and cytokines as Schwann cells and signaling through critical neurotrophic pathways.

Usage: Immunofluorescence

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)