Serre NBC, Sarthou M, Gigarel O, Figuet S, Corso M, Choulet J, Rofidal V, Alban C, Santoni V, Bourguignon J, Verbruggen N, Ravanel S (2020) Protein lysine methylation contributes to modulating the response of sensitive and tolerant Arabidopsis species to cadmium stress. Plant Cell Environ 43(3):760-774. doi: 10.1111/pce.13692 PMID: 31759334
Wu X, Cao Z, Chen H, Ou Q, Huang X, Wang Y (2020) Downregulation of Linc-RNA activator of myogenesis lncRNA participates in FGF2-mediated proliferation of human periodontal ligament stem cells. J Periodontol 91(3):422-427. doi: 10.1002/JPER.19-0317 PMID: 31378921
Cutuli D, Landolfo E, Decandia D, Nobili A, Viscomi MT, La Barbera L, Sacchetti S, De Bartolo P, Curci A, D’Amelio M, Farioli-Vecchioli S, Petrosini L (2020) Neuroprotective role of dietary supplementation with omega-3 fatty acids in the presence of basal forebrain cholinergic neurons degeneration in aged mice. Int J Mol Sci 21(5):1741. doi: 10.3390/ijms21051741 PMID: 32143275
Objective: Examine the neuroprotective effects of omega-3 polyunsaturated fatty acids in an Alzheimer’s model of aged mice.
Summary: Aged mice were fed omega-3 polyunsaturated fatty acids (n-3 PUFA) or a caloric-equivalent source of fat without n-3 PUFA. Afterward, mice were subjected to lesioning of the cholinergic system with mu p-75-SAP (Cat. IT-16) and assessed for dementia-like symptoms. Mice fed with n-3 PUFA performed better on memory retention tasks and had more exploratory behaviors.
Usage: Intracerebroventricular (i.c.v.) injections of mu-p75-saporin (IT-16); total dosage 0.6 μg/mouse.
Zhang F, Huan L, Xu T, Li G, Zheng B, Zhao H, Guo Y, Shi J, Sun J, Chen A (2020) Inflammatory macrophages facilitate mechanical stress-induced osteogenesis. Aging (Albany NY) 12(4):3617-3625. doi: 10.18632/aging.102833
Summary: In a mouse model of distraction osteogenesis (DO), there was significant increase in macrophages in the regeneration area. This suggests that targeting inflammatory macrophages may help to improve clinical bone repair.
Usage: For saporin-mediated depletion of macrophages, DO-surgery-treated mice received an intraventricular (iv) injection of either Mac-1-SAP or Rat IgG-SAP (20µg) once every 3 days.
Li S, Zhao W, Tao Y, Liu C (2020) Fugan Wan alleviates hepatic fibrosis by inhibiting ACE/Ang II/AT-1R signaling pathway and enhancing ACE2/Ang 1-7/Mas signaling pathway in hepatic fibrosis rat models. Am J Transl Res 12(2):592-601. PMID: 32194907
Objective: To investigate protective effects of Fugan Wan (FGW) on hepatic fibrosis and clarify associated mechanisms.
Summary: FGW alleviated hepatic fibrosis by inhibiting ACE/Ang II/AT-1R signaling and enhancing ACE2/Ang 1-7/Mas signaling pathway in hepatic fibrosis rat models.
Meng J, Chen W, Wang J (2020) Intervening B-type natriuretic peptide signaling for controlling chronic itch. Brit J Pharmacol 177(5):1025-1040. doi: 10.1111/bph.14952
Objective: Review of recent findings used to examine the role of B-type natriuretic peptide (BNP) in itch transduction and the modulation of other pururitic proteins.
Summary: Mice treated with Nppb-SAP ablated 70% of the BNP receptor-positive neurons in the spinal cord.
Srikanthan MA, Humbert O, Haworth KG, Ironside C, Rajawat YS, Blazar BR, Palchaudhuri R, Boitano AE, Cooke MP, Scadden DT, Kiem HP (2020) Effective multi-lineage engraftment in a mouse model of fanconi anemia using non-genotoxic antibody-based conditioning. Mol Ther Methods Clin Dev 17:455-464. doi: 10.1016/j.omtm.2020.02.001 PMID: 32226796
Objective: To utilize antibody-drug conjugates (ADC) as an alternative conditioning regimen in a Fanconi anemia (FA) mouse model of autologous transplantation.
Summary: Antibody conjugates targeting hematopoietic cells are an emerging non-genotoxic method of promoting engraftment of transplanted cells while maintaining intact marrow cellularity. FANCA knockout mice were conditioned with either Anti-CD45-SAP or Anti-CD117-SAP prior to receiving whole marrow from a heterozygous healthy donor. Bone marrow and peripheral blood analysis revealed equivalent levels of donor engraftment, with minimal toxicity in ADC-treated groups as compared with cyclophosphamide-treated control.
Usage: Anti-CD45-SAP (3 mg/kg total complex) or Anti-CD117-SAP (1.5 mg/kg total complex) via tail vein injection.
Crevier-Sorbo G, Rymar, VV,Crevier-Sorbo R, Sadikot AF (2020) Thalamostriatal degeneration contributes to dystonia and cholinergic interneuron dysfuntion in a mouse model of huntington’s disease. Acta Neruopatho Commun 8(1):14. doi: 10.1186/s40478-020-0878-0 PMID: 32033588
Objective: To ablate the neurons of the Thalamostrial system (TS) to elucidate their role in the motor symptoms of Huntington’s Disease.
Summary: Huntington’s disease is an autosomal disorder characterized by involuntary movement and striatal neuronal loss. Glutaminergic input from the TS is implicated in disease progression and motor deficits. Anti-ChAT-SAP is used to ablate neurons in the Thalamostrial system to understand the role these neurons played in Huntington’s.
Usage: Mice underwent unilateral, striatal injections with either Anti-ChAT-SAP (IT-42) or Rabbit IgG-SAP (IT-35). The total volume and concentration of either saporin construct was the same (0.7 μL of 0.6 μg/μL solution).
Cui LJ, Chen WH, Liu AL, Han X, Jiang SX, Yuan F, Zhong YM, Yang XL, Weng SJ (2020) nGnG amacrine cells and Brn3b-negative M1 ipRGCs are specifically labeled in the ChAT-ChR2-EYFP mouse. Invest Ophthalmol Vis Sci 61(2):14. doi: 10.1167/iovs.61.2.14 PMID: 32049344
Summary: The authors speculated that type II cells might be ipRGCs. This was later verified by the strong immunostaining of type II cells in response to the melanopsin antibody UF006 (100%, 141 of 141 cells collected from 5 retinas, Figs. 6B1–B3), which probes multiple ipRGC subtypes.
