References

Nattie E, Li A (2006) Neurokinin-1 receptor expressing neurons in the ventral medulla are essential for normal central and peripheral chemoreception in the conscious rat. J Appl Physiol 101(6):1596-1606. doi: 10.1152/japplphysiol.00347.2006

Summary: All known chemoreceptor sites in the mammalian brainstem are rich in the neurokinin-1 receptor (NK1r). The authors ask if these cells scattered throughout the ventral medulla are involved in central and peripheral chemoreception. Rats received 250-280 ng of SSP-SAP (Cat. #IT-11) into the cisterna magna, mouse IgG-SAP (Cat. #IT-18) was used as a control. The results indicate that NK1r neurons in the ventral medulla are involved in both central and peripheral chemoreception, during both waking and sleep states.

Related Products: SSP-SAP (Cat. #IT-11), Mouse IgG-SAP (Cat. #IT-18)

Adam PJ, Terrett JA, Steers G, Stockwin L, Loader JA, Fletcher GC, Lu LS, Leach BI, Mason S, Stamps AC, Boyd RS, Pezzella F, Gatter KC, Harris AL (2006) CD70 (TNFSF7) is expressed at high prevalence in renal cell carcinomas and is rapidly internalised on antibody binding. Br J Cancer 95(3):298-306. doi: 10.1038/sj.bjc.6603222

Summary: Renal cell carcinoma (RCC) is usually resistant to chemotherapy. Using a proteomics approach, the authors found a potential target for immunotherapy in RCC. An antibody against CD70, a type II transmembrane receptor, was combined with Hum-ZAP (Cat. #IT-22). The complex was then added to an RCC-derived cell-line in vitro. The aCD70/Hum-ZAP complex demonstrated significant killing at several different concentrations. This work suggests that CD70 is a potential target antigen for RCC therapy.

Related Products: Hum-ZAP (Cat. #IT-22)

Zhu JP, Xu W, Angulo JA (2006) Distinct mechanisms mediating methamphetamine-induced neuronal apoptosis and dopamine terminal damage share the neuropeptide substance p in the striatum of mice. Ann N Y Acad Sci 1074:135-148. doi: 10.1196/annals.1369.013 PMID: 17105911

Objective: To investigate the mechanism by which substance P mediates METH-induced damage.

Summary: The authors propose that substance P mediates the apoptosis of some striatal neurons via the intrastriatal activation of nitric oxide synthesis. Substance P may also mediate damage of the dopamine terminals via an extrastriatal mechanism involving the substantia nigra and cortical glutamate release.

Usage: Mice were given intrastriatal injections of SSP-SAP (4ng/mcl). Saporin was used as control.

Related Products: SSP-SAP (Cat. #IT-11), Saporin (Cat. #PR-01)

Blanco-Centurion C, Xu M, Murillo-Rodriguez E, Gerashchenko D, Shiromani AM, Salin-Pascual RJ, Hof PR,Shiromani PJ (2006) Adenosine and sleep homeostasis in the basal forebrain. J Neurosci 26(31):8092-8100. doi: 10.1523/JNEUROSCI.2181-06.2006

Summary: It has been shown that adenosine induces sleep and levels of adenosine increase during times of wakefulness. The authors investigated whether basal forebrain cholinergic neurons are involved in adenosine regulation of sleep. 6 µg of 192-IgG-SAP (Cat. #IT-01) was administered to the lateral ventricle of rats. In treated animals, adenosine levels did not increase with prolonged waking. The treated animals did, however, retain intact sleep drive.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Momiyama T, Zaborszky L (2006) Somatostatin presynaptically inhibits both GABA and glutamate release onto rat basal forebrain cholinergic neurons. J Neurophysiol 96(2):686-694. doi: 10.1152/jn.00507.2005 PMID: 16571735

Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #AB-N43FL3)

Vera-Portocarrero LP, Zhang ET, Ossipov MH, Xie JY, King T, Lai J, Porreca F (2006) Descending facilitation from the rostral ventromedial medulla maintains nerve injury-induced central sensitization. Neuroscience 140(4):1311-1320. doi: 10.1016/j.neuroscience.2006.03.016

Summary: Rats were treated with 1.5 pmol of dermorphin-SAP (Cat. #IT-12) or saporin (Cat. #PR-01) into each side of the rostral ventromedila medulla, followed by spinal nerve ligation. The data indicate that mu opioid-expresing neurons are necessary to maintain nerve injury-induced central sensitization.

Related Products: Dermorphin-SAP / MOR-SAP (Cat. #IT-12), Saporin (Cat. #PR-01)

Ledri M, Cusulin C, Novati A, Aztiria E, Leanza G (2006) Selective cholinergic immunolesioning in alpha7 nAChR KO mice: Anatomical, neurochemical and functional effects. FENS 2006 Abstracts 3:A1610.10. Federation of European Neuroscience Societies, Vienna, Austria.

Related Products: mu p75-SAP (Cat. #IT-16)

Singh B, Henneberger C, Betances D, Arevalo MA, Rodríguez-Tébar A, Meier JC, Grantyn R (2006) Altered balance of glutamatergic/GABAergic synaptic input and associated changes in dendrite morphology after BDNF expression in BDNF-deficient hippocampal neurons. J Neurosci 26(27):7189-7200. doi: 10.1523/JNEUROSCI.5474-05.2006 PMID: 16822976

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01), NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Allen JW (2006) Featured Article: Safety and efficacy of Substance P-SAP. Targeting Trends 7(3)

Related Products: SP-SAP (Cat. #IT-07), Saporin (Cat. #PR-01)

Read the featured article in Targeting Trends.

See Also:

• Allen JW et al. Safety evaluation of Intrathecal Substance P-Saporin, a targeted neurotoxin, in dogs. Toxicol Sci 91(1):286-298, 2006.

Fraley GS (2006) Immunolesions of glucoresponsive projections to the arcuate nucleus alter glucoprivic-induced alterations in food intake, luteinizing hormone secretion, and GALP mRNA, but not sex behavior in adult male rats. Neuroendocrinology 83(2):97-105. doi: 10.1159/000094375

Summary: In this work the author looked at the role hypothalamic glucose may play in reproductive function. 42 ng of anti-DBH-SAP (Cat. #IT-03) was injected dorsal of the arcuate nucleus of rats, which were then given glucoprivic challenges. Feeding and sex behavior were decreased during glucoprivation; sex behavior was also decreased in control animals. The data demonstrate the involvement of A1/C1 efferents to the ventromedial hypothalamus in glucostatic regulation of various processes.

Related Products: Anti-DBH-SAP (Cat. #IT-03)