References

Vera-Portocarrero LP, Zhang ET, Ossipov MH, Xie JY, King T, Lai J, Porreca F (2006) Descending facilitation from the rostral ventromedial medulla maintains nerve injury-induced central sensitization. Neuroscience 140(4):1311-1320. doi: 10.1016/j.neuroscience.2006.03.016

Summary: Rats were treated with 1.5 pmol of dermorphin-SAP (Cat. #IT-12) or saporin (Cat. #PR-01) into each side of the rostral ventromedila medulla, followed by spinal nerve ligation. The data indicate that mu opioid-expresing neurons are necessary to maintain nerve injury-induced central sensitization.

Related Products: Dermorphin-SAP / MOR-SAP (Cat. #IT-12), Saporin (Cat. #PR-01)

Ledri M, Cusulin C, Novati A, Aztiria E, Leanza G (2006) Selective cholinergic immunolesioning in alpha7 nAChR KO mice: Anatomical, neurochemical and functional effects. FENS 2006 Abstracts 3:A1610.10. Federation of European Neuroscience Societies, Vienna, Austria.

Related Products: mu p75-SAP (Cat. #IT-16)

Singh B, Henneberger C, Betances D, Arevalo MA, Rodríguez-Tébar A, Meier JC, Grantyn R (2006) Altered balance of glutamatergic/GABAergic synaptic input and associated changes in dendrite morphology after BDNF expression in BDNF-deficient hippocampal neurons. J Neurosci 26(27):7189-7200. doi: 10.1523/JNEUROSCI.5474-05.2006 PMID: 16822976

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01), NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Allen JW (2006) Featured Article: Safety and efficacy of Substance P-SAP. Targeting Trends 7(3)

Related Products: SP-SAP (Cat. #IT-07), Saporin (Cat. #PR-01)

Read the featured article in Targeting Trends.

See Also:

• Allen JW et al. Safety evaluation of Intrathecal Substance P-Saporin, a targeted neurotoxin, in dogs. Toxicol Sci 91(1):286-298, 2006.

Fraley GS (2006) Immunolesions of glucoresponsive projections to the arcuate nucleus alter glucoprivic-induced alterations in food intake, luteinizing hormone secretion, and GALP mRNA, but not sex behavior in adult male rats. Neuroendocrinology 83(2):97-105. doi: 10.1159/000094375

Summary: In this work the author looked at the role hypothalamic glucose may play in reproductive function. 42 ng of anti-DBH-SAP (Cat. #IT-03) was injected dorsal of the arcuate nucleus of rats, which were then given glucoprivic challenges. Feeding and sex behavior were decreased during glucoprivation; sex behavior was also decreased in control animals. The data demonstrate the involvement of A1/C1 efferents to the ventromedial hypothalamus in glucostatic regulation of various processes.

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Garrett JE, Kim I, Wilson RE, Wellman CL (2006) Effect of N-methyl-d-aspartate receptor blockade on plasticity of frontal cortex after cholinergic deafferentation in rat. Neuroscience 140(1):57-66. doi: 10.1016/j.neuroscience.2006.01.029

Summary: Acetylcholine from the nucleus basalis magnocellularis (NBM) plays roles in neocortical function and plasticity. Here the authors examined whether N-methyl-D-aspartate receptors mediate the increase in the GluR1 subunit of the a-amino-3-hydroxy-5-methylisoxazole-4-proprionate receptor in the frontal cortex following treatment of the NBM with 0.15 µg of 192-IgG-SAP (Cat. #IT-01). The data indicates that upregulation of GluR1 and spine density after cholinergic deafferentation is regulated by N-methyl-D-aspartate receptors.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Kanai T, Uraushihara K, Totsuka T, Nemoto Y, Fujii R, Kawamura T, Makita S, Sawada D, Yagita H, Okumura K, Watanabe M (2006) Ameliorating effect of saporin-conjugated anti-CD11b monoclonal antibody in a murine T-cell-mediated chronic colitis. J Gastroenterol Hepatol 21(7):1136-1142. doi: 10.1111/j.1440-1746.2006.04391.x

Summary: Crohn’s disease is characterized by massive infiltration of macrophages and CD4(+) T-cells in the colon and small intestine. Using SCID mice, the authors evaluated the effects of Mac-1-SAP (Cat. #IT-06) on the development of chronic colitis. After transfer of T cells to the mice, 12.5 µg of Mac-1-SAP was injected into the intraperitoneal space. The reduction in CD4(+) T-cell infiltration of the colon, and suppression of IFNg and TNFa production indicate that macrophages play a significant role in the pathogenesis of Crohn’s disease.

Related Products: Mac-1-SAP mouse/human (Cat. #IT-06)

Traissard N, Herbeaux K, Cosquer B, Jeltsch H, Ferry B, Galani R, Pernon A, Majchrzak M, Cassel JC (2007) Combined damage to entorhinal cortex and cholinergic basal forebrain neurons, two early neurodegenerative features accompanying Alzheimer’s Disease: Effects on locomotor activity and memory functions in rats. Neuropsychopharmacology 32(4):851-871. doi: 10.1038/sj.npp.1301116

Summary: Two characteristics of Alzheimer’s disease (AD) are cholinergic dysfunction in the basal forebrain, and neuronal damage in the entorhinal cortex. Using 5 µg intracerebroventricular (icv) injections of 192-IgG-SAP (Cat. #IT-01), and 2.3 µg icv injections of OX7-SAP (Cat. #IT-02), locomotor activity, working, and reference memory of rats were examined. Although 192-IgG-SAP lesions caused limited deficits, rats receiving both lesions exhibited several behaviors associated with AD. The authors suggest that combining these lesions may be a more accurate model for AD than 192-IgG-SAP alone.

Related Products: 192-IgG-SAP (Cat. #IT-01), OX7-SAP (Cat. #IT-02)

Nguyen DH, Ball ED, Varki A (2006) Myeloid precursors and acute myeloid leukemia cells express multiple CD33-related Siglecs. Exp Hematol 34(6):728-735. doi: 10.1016/j.exphem.2006.03.003

Summary: Sialic acid-binding immunoglobulin-like lectins (Siglecs) are a family of cell surface receptors which bind to sialic acid. They are found mainly on leukocytes, and also on acute myeloid leukemia (AML) cells. The authors tested several anti-Siglec antibodies against U937 histiocytic lymphoma cells and THP-1 acute monocytic leukemia cells in vitro. When these antibodies were combined with Mab-ZAP (Cat. #IT-04), a second immunotoxin, the target cells were eliminated. The data suggest that Siglecs may be a viable target for AML therapy.

Related Products: Mab-ZAP (Cat. #IT-04)

Vera-Portocarrero LP, Yie JX, Kowal J, Ossipov MH, King T, Porreca F (2006) Descending facilitation from the rostral ventromedial medulla maintains visceral pain in rats with experimental pancreatitis. Gastroenterology 130(7):2155-2164. doi: 10.1053/j.gastro.2006.03.025

Summary: Here the authors investigated the role of ascending or descending pathways in the mediation of pain caused by pancreatitis. Rats received 1.5 pmol injections of dermorphin-SAP (Cat. #IT-12) into each side of the rostral ventromedial medulla. Abdominal hypersensitivity was tested using von Frey filaments. Although the ablation of mu-opioid receptor-expressing neurons by dermorphin-SAP did not prevent the initial expression of pancreatitis pain, maintenance of this pain was absent. The data link maintenance of pancreatitis pain to descending pathways.

Related Products: Dermorphin-SAP / MOR-SAP (Cat. #IT-12)