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Featured Article: Selective ablation of IB4+ primary afferent neurons reduces mechanical and cold hyperalgesia in an EAE mouse model of multiple sclerosis
Nguyen KL, Lamerand SR, Deshpande RP, Taylor BK (2021) Featured Article: Selective ablation of IB4+ primary afferent neurons reduces mechanical and cold hyperalgesia in an EAE mouse model of multiple sclerosis. Targeting Trends 22
Related Products: IB4-SAP (Cat. #IT-10), Blank-SAP (Cat. #IT-21)
Read the featured article in Targeting Trends.
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A spinal microglia population involved in remitting and relapsing neuropathic pain
Kohno K, Shirasaka R, Yoshihara K, Mikuriya S, Tanaka K, Takanami K, Inoue K, Sakamoto H, Ohkawa Y, Masuda T, Tsuda M (2022) A spinal microglia population involved in remitting and relapsing neuropathic pain. Science 376(6588):86-90. doi: 10.1126/science.abf6805
Objective: To investigate pain recovery mechanisms.
Summary: The authors reveal a mechanism for the remission and recurrence ofneuropathic pain, providing potential targets for therapeutic strategies.
Usage: The dose of CTB-SAP and IB4-SAP was 8 ug/10 uL, diluted in PBS.
Related Products: CTB-SAP (Cat. #IT-14), IB4-SAP (Cat. #IT-10)
Upregulation of p75NTR by histone deacetylase inhibitors sensitizes human neuroblastoma cells to targeted immunotoxin-induced apoptosis
Dedoni S, Olianas A, Manconi B, Collu M, Tuveri B, Vincis ME, Olianas MC, Onali P (2022) Upregulation of p75NTR by histone deacetylase inhibitors sensitizes human neuroblastoma cells to targeted immunotoxin-induced apoptosis. Int J Mol Sci 23(7):3849. doi: 10.3390/ijms23073849 PMID: 35409209
Summary: Histone deacetylase (HDAC) inhibitors have been useful chemotherapy agents in the treatment of neuroblastomas, the most frequent solid tumor of childhood. Studies have shown that the expression of the neurotrophin receptor, p75NTR, on human neuroblastoma cells are enhanced after exposure to some HDAC inhibitors. The authors used mu p75-SAP along with two HDAC inhibitors, valproic acid and entinostat, to investigate if they could exploit the upregulation of p75NTR and see if these cells were more susceptible as a target for elimination. The administration of mu p75-SAP only induced cell death in tumors of mice that were pretreated with entinostat
Usage: Cells were treated for 24 hr with entinostat and then exposed to 30 nM of mu p75-SAP for 24 hr.
Related Products: mu p75-SAP (Cat. #IT-16)
Urokinase-type plasminogen activator receptor (uPAR) as a therapeutic target in cancer
Zhai BT, Tian H, Sun J, Zou JB, Zhang XF, Cheng JX, Shi YJ, Fan Y, Guo DY (2022) Urokinase-type plasminogen activator receptor (uPAR) as a therapeutic target in cancer. J Translational Medicine 20:135. doi: 10.1186/s12967-022-03329-3 PMID: 35303878
Summary: The authors describe how a conjugate of Saporin and ATF (Urokinase-type plasminogen activator receptor (uPAR) as a therapeutic target in cancer amino‐terminal fragment of urokinase) exerts antitumor effects by targeting Urokinase-type plasminogen activator receptor (uPAR).
A CD45-targeted antibody-drug conjugate successfully conditions for allogeneic hematopoietic stem cell transplantation in mice
Saha A, Hyzy S, Lamothe T, Hammond K, Clark N, Lanieri L, Bhattarai P, Palchaudhuri R, Gillard GO, Proctor J, Riddle MJ, Panoskaltsis-Mortari A, MacMillan ML, Wagner JE, Kiem HP, Olson LM, Blazar BR (2022) A CD45-targeted antibody-drug conjugate successfully conditions for allogeneic hematopoietic stem cell transplantation in mice. Blood 139(11):1743-1759. doi: 10.1182/blood.2021012366 PMID: 34986233
Objective: To investigate the effectiveness of a CD45-targeted antibody-drug conjugate (ADC) in conditioning for allogeneic hematopoietic stem cell transplantation (HSCT) in mice.
Summary: In this study, researchers evaluated a novel CD45-targeted antibody-drug conjugate as a conditioning regimen for allogeneic hematopoietic stem cell transplantation in mice. The results demonstrated successful conditioning, highlighting the potential of this approach for improving the outcomes of allogeneic HSCT in the future.
Related Products: Anti-CD117-SAP (Cat. #IT-83)
See Also:
- Palchaudhuri R et al. Non-genotoxic conditioning for hematopoietic stem cell transplantation using a hematopoietic-cell-specific internalizing immunotoxin. Nat Biotechnol 34:738-745, 2016.
- Czechowicz A et al. Selective hematopoietic stem cell ablation using CD117-antibody-drug-conjugates enables safe and effective transplantation with immunity preservation. Nat Commun 10:617, 2019.
- Li Z et al. Hematopoietic chimerism and donor-specific skin allograft tolerance after non-genotoxic CD117 antibody-drug-conjugate conditioning in MHC-mismatched allotransplantation. Nat Commun 10:616, 2019.
- Gao C et al. Nongenotoxic antibody-drug conjugate conditioning enables safe and effective platelet gene therapy of hemophilia A mice. Blood Adv 3(18):2700-2711, 2019.
- Castiello MC et al. Efficacy and safety of anti-CD45-saporin as conditioning agent for RAG deficiency. J Allergy Clin Immunol 147(1):309-320.e6, 2021.
Immunological barriers to haematopoietic stem cell gene therapy
Charlesworth CT, Hsu I, Wilkinson AC, Nakauchi H (2022) Immunological barriers to haematopoietic stem cell gene therapy. Nat Rev Immunol 1-15. doi: 10.1038/s41577-022-00698-0
Objective: This review article attempts to encourage more research to address the immunological barriers to haematopoietic stem cell based gene therapies.
Summary: The authors lay out the history and clinical trials of hematopoietic stem cell gene therapy and then discuss the challenges of both innate immunity and adaptive immunity.
Usage: This review mentions a publication that used Anti-CD117-SAP to ablate hematopoietic stem cells.
Related Products: Anti-CD117-SAP (Cat. #IT-83)
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Noradrenergic innervations of the medial prefrontal cortex mediate empathy for pain in rats via the α1 and β receptors.
Yun-Fei L, Zhang J, Wang XQ, Peng JJ, Ling BF, Liu FT, Yang F, Dong G, Yu YQ (2022) Noradrenergic innervations of the medial prefrontal cortex mediate empathy for pain in rats via the α1 and β receptors. Behav Brain Res 10:113828. doi: 10.1016/j.bbr.2022.113828
Objective: To study the roles of the locus coeruleus (LC) to medial prefrontal cortex (mPFC) pathway in pain empathy in rats.
Summary: Results indicate that noradrenergic innervations in the mPFC mediate empathy for pain in rats via the α1 and β receptors.
Usage: Noradrenergic innervations of the mPFC were selectively eliminated through intra-mPFC injections of Anti-DBH-SAP.
Related Products: Anti-DBH-SAP (Cat. #IT-03)
Protective Effects of a synthetic glycosaminoglycan mimetic (OTR4132) in a rat immunotoxic lesion model of septohippocampal cholinergic degeneration
Pereira PM, Papy-Garcia D, Barritault D, Chiappini F, Jackisch R, Schimchowitsch S, Cassel JC (2022) Protective Effects of a synthetic glycosaminoglycan mimetic (OTR4132) in a rat immunotoxic lesion model of septohippocampal cholinergic degeneration. Glycoconj J 39(1):107-130. doi: 10.1007/s10719-022-10047-x PMID: 35254602
Objective: Assess the effects of OTR4132, a synthetic heparan-mimetic biopolymer that is designed to have neuroprotective/neurotrophic properties.
Summary: 192-IgG-SAP was used to create a partial hippocampal cholinergic denervation model. Rats were also injected with OTR4132, either intramuscularly (1.5 mg/kg) or into the lateral ventricle (0.25ug/5 ul/rat). Rats injected with 192-IgG-SAP showed decreases in (1) hippocampal acetylcholinesterase reaction products and in (2) choline acetyltransferase-positive neurons in the medial septum. Both these attributes were significantly lessened in rats treated with OTR4132.
Usage: 192-IgG-SAP was injected into the medial septum/diagonal band of broca (0.37 ug) of Long-Evans male rats.
Related Products: 192-IgG-SAP (Cat. #IT-01)
Angiotensin II enhances group 2 innate lymphoid cell responses via AT1a during airway inflammation.
Liu G, Chen Y, Wang Y, Deng X, Xiao Q, Zhang L, Xu H, Han X, Lei A, He J, Li X, Cao Y, Zhou P, He C, Wu P, Jiang W, Tan M, Chen C, Yang Q, Lu L, Deng K, Yao Z, Zhou J (2022) Angiotensin II enhances group 2 innate lymphoid cell responses via AT1a during airway inflammation. J Exp Med 219(3):e20211001. doi: 10.1084/jem.20211001 PMID: 35044462
Objective: To identify angiotensin (Ang) II as a positive regulator of Group 2 innate lymphoid cells (ILC2s).
Summary: Ang II plays a critical role in regulating ILC2 responses and airways inflammation.
Usage: Administration of Ang II (30ug) i.n. or i.p. daily for 5 days.
Related Products: Angiotensin II receptor (AT-1AR) Rabbit Polyclonal, affinity-purified (Cat. #AB-N25AP)
Local renin angiotensin system and sperm DNA fragmentation
Aparicio Prieto MV, Rodríguez Gallego MV, Valdivia Palacín A, Franco Iriarte Y, Hervás Barbara G, Echevarría Orella E, Casis Saenz L (2022) Local renin angiotensin system and sperm DNA fragmentation. Asian J Androl 24(2):139-146. doi: 10.4103/aja202150 PMID: 34494558
Objective: The renin angiotensin system (RAS) appears to influence male fertility at multiple levels. The relationship between the RAS and DNA integrity was analyzed.
Summary: Fragmented DNA spermatozoa have a lower capacity to respond to bioactive RAS peptides.
Usage: Immunocytochemistry
Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)