Gupta S, Kesler MT, Scantlebury MH, Sloviter RS, Teskey GC (2026) Modeling temporal lobe epilepsy with hippocampal sclerosis in rats using the selective neurotoxin stable substance P-saporin. Epilepsia doi: 10.1002/epi.70322 PMID: 42220243
Objective: To determine the time course of SSP-SAP-induced epileptogenesis, as well as the loss of principal cells and astrogliosis, two defining features of Temporal lobe epilepsy with hippocampal sclerosis (TLE- HS+)
Summary: Temporal lobe epilepsy with hippocampal sclerosis (TLE- HS+) is a common and often refractory form of human epilepsy. SSP-SAP was internalized within 2 h after injection and was immunocytochemically undetectable by 5 days. Rats exhibited spontaneous electrographic and behavioral reactive seizures between days 4 and 6 after SSP-SAP injection, with most seizures having a Racine score of either 1 or 2. This study confirms that the SSP- SAP model reproduces the defining features of human TLE and that a primary, selective, and longitudinally extensive γ- aminobutyric acidergic defect is sufficient to trigger epileptogenesis that results in TLE-HS+.
Usage: 150 nL of SSP-SAP (0.04 ng/nL, IT-11) was injected at a rate of 1 nL/s into four unilateral sites along the longitudinal axis of the rat dentate gyrus. To determine the time course for SSP- SAP internalization (n=4), rats were perfused at 2 h and 1, 3, and 5 days following SSP- SAP administration. Following coronal cryosectioning of the hippocampus, sections were placed in a 500-μL solution of 1:350 Saporin Goat Polyclonal, affinity-purified Alexa 488 (AB-15AP-FLA) and .01 mol·L−1 PBS at room temperature overnight. Sections were inspected and imaged at 40× with 4× digital zoom using an Olympus Fluoview FV3000 confocal microscope.
Related Products: SSP-SAP (Cat. #IT-11), Saporin Goat Polyclonal, affinity-purified Alexa488-labeled (Cat. #AB-15AP-FLA)