Hoffmann RM, Mele S, Cheung A, Larcombe-Young D, Bucaite G, Sachouli E, Zlatareva I, Morad HOJ, Marlow R, McDonnell JM, Figini M, Lacy KE, Tutt AJN, Spicer JF, Thurston DE, Karagiannis SN, Crescioli S (2020) Rapid conjugation of antibodies to toxins to select candidates for the development of anticancer antibody-drug conjugates (ADCs). Sci Rep 10(1):8869. doi: 10.1038/s41598-020-65860-x
Objective: To facilitate rapid and cost-effective screening of antibody and toxin combinations for activity and safety, based on streptavidin-biotin conjugation
Summary: For antibody selection, the authors evaluated internalization by target cells using streptavidin-linked antibodies conjugated to biotinylated saporin, a toxin unable to cross cell membranes. For payload selection, the authors biotinylated toxins and conjugated them to antibodies linked to streptavidin to evaluate antitumour activity and pre-clinical safety.
Usage: Streptavidin-linked antibody was incubated for 30min with biotinylated Saporin (BT-ZAP) at a molar ratio of 1:6.
Related Products: Streptavidin-ZAP (Cat. #IT-27), MonoBiotin-ZAP (Cat. #BT-ZAP)