Advanced Targeting Systems announces the release of two reagents for immunological studies: Anti-Tac (Cat. # AB-18) – a monoclonal antibody to human CD25, the interleukin-2 receptor, and Anti-Tac-SAP (Cat. # IT-24) – the immunotoxin made by conjugation of anti-Tac to saporin. These reagents add to the growing list of ATS products for use in studies […]
Targeting Tools: Anti-Tac and Anti-Tac-SAP Read More »
Johnson DA, Zambon NJ, Gibbs RB (2002) Selective lesion of cholinergic neurons in the medial septum by 192 IgG-saporin impairs learning in a delayed matching to position T-maze paradigm. Brain Res 943(1):132-141. doi: 10.1016/s0006-8993(02)02623-9 Summary: The authors investigated the effects of selective cholinergic depletion in the medial septum on a spatial memory (DMP) task. Direct
Lehmann O, Jeltsch H, Lazarus C, Tritschler L, Bertrand F, Cassel JC (2002) Combined 192 IgG-saporin and 5,7-dihydroxytryptamine lesions in the male rat brain: A neurochemical and behavioral study. Pharmacol Biochem Behav 72(4):899-912. doi: 10.1016/s0091-3057(02)00752-9 Summary: Female rats treated with both 192-Saporin (Cat #IT-01) and 5,7-DHT exhibited working memory impairments that were not seen with
Lehmann O, Bertrand F, Jeltsch H, Morer M, Lazarus C, Will B, Cassel JC (2002) 5,7-DHT-induced hippocampal 5-HT depletion attenuates behavioural deficits produced by 192 IgG-saporin lesions of septal cholinergic neurons in the rat. Eur J Neurosci 15(12):1991-2006. doi: 10.1046/j.1460-9568.2002.02037.x Summary: Although past data has shown that the cholinergic system of the basal forebrain is
Contributed by David J Flavell, PhD, The Simon Flavell Leukaemia Research Unit, Division of Cancer Sciences, University of Southampton Medical School, United Kingdom. I have asked Dr. Flavell to contribute an article on the clinical use of his saporin immunotoxins. Dr. Flavell’s research unit is named after his son, who died from leukemia. At that
Cover Article: Saporin Immunotoxins for Treating Human Hematological Malignancies Read More »
Easton A, Ridley RM, Baker HF, Gaffan D (2002) Unilateral lesions of the cholinergic Basal forebrain and fornix in one hemisphere and inferior temporal cortex in the opposite hemisphere produce severe learning impairments in rhesus monkeys. Cereb Cortex 12(7):729-736. doi: 10.1093/cercor/12.7.729 Summary: The authors used a combination of basal forebrain lesioning using ME20.4-SAP (Cat. #IT-15)
Porreca F, Ossipov MH, Gebhart GF (2002) Chronic pain and medullary descending facilitation. Trends Neurosci 25(6):319-325. doi: 10.1016/s0166-2236(02)02157-4 Objective: To examine the likelihood that sustained activation of descending modulatory pathways that facilitate pain transmission could underlie some states of chronic pain. Summary: Rats treated with Dermorphin-SAP, either before or after spinal nerve ligation injury, did
Chronic pain and medullary descending facilitation Read More »
Burgess SE, Gardell LR, Ossipov MH, Malan Jr TP, Vanderah TW, Lai J, Porreca F (2002) Time-dependent descending facilitation from the rostral ventromedial medulla maintains, but does not initiate, neuropathic pain. J Neurosci 22(12):5129-5136. doi: 10.1523/JNEUROSCI.22-12-05129.2002 Summary: Various indications, such as declining afferent discharge over time, suggest that the mechanisms involved in persistent neuropathic pain
Risbrough V, Bontempi B, Menzaghi F (2002) Selective immunolesioning of the basal forebrain cholinergic neurons in rats: effect on attention using the 5-choice serial reaction time task. Psychopharmacology 164:71-81. doi: 10.1007/s00213-002-1170-7 Summary: The authors used 0.067 µg injections of 192-Saporin (Cat. #IT-01) into the nucleus basalis magnocellularis to investigate attentional performance in rats. The treated
Galani R, Jeltsch H, Lehmann O, Bertrand F, Cassel JC (2002) Effects of 192 IgG-saporin on acetylcholinesterase histochemistry in male and female rats. Brain Res Bull 58(2):179-186. doi: 10.1016/s0361-9230(02)00775-x Summary: Male rats were treated with estradiol, and 2-µg i.c.v. injections of 192-Saporin (Cat #IT-01). Related Products: 192-IgG-SAP (Cat. #IT-01)