Kristen Hartman – Page 215 – Advanced Targeting Systems

Transplant of GABAergic precursors restores hippocampal inhibitory function in a mouse model of seizure susceptibility.

Zipancic I, Calcagnotto ME, Piquer-Gil M, Mello LE, Alvarez-Dolado M (2010) Transplant of GABAergic precursors restores hippocampal inhibitory function in a mouse model of seizure susceptibility. Cell Transplant 19(5):549-564. doi: 10.3727/096368910X491383 Summary: Although medial ganglionic eminence-derived cells can be grafted into the neonatal brain and become functionally mature GABAergic neurons it is not clear whether […]

Transplant of GABAergic precursors restores hippocampal inhibitory function in a mouse model of seizure susceptibility. Read More »

Recent progress in research on ribosome inactivating proteins.

Kristen Hartman

Ng TB, Wong JH, Wang H (2010) Recent progress in research on ribosome inactivating proteins. Curr Protein Pept Sci 11(1):37-53. doi: 10.2174/138920310790274662 Summary: This review discusses recent literature on ribosome inactivating proteins including the use of saporin-based conjugates in neuroscience and cancer research. Brief descriptions of research done using 192-IgG-SAP (Cat. #IT-01), OX7-SAP (Cat. #IT-02),

Recent progress in research on ribosome inactivating proteins. Read More »

The cerebellum harbors a circadian oscillator involved in food anticipation.

Kristen Hartman

Mendoza J, Pevet P, Felder-Schmittbuhl MP, Bailly Y, Challet E (2010) The cerebellum harbors a circadian oscillator involved in food anticipation. J Neurosci 30:1894-1904. doi: 10.1523/JNEUROSCI.5855-09.2010 Summary: The authors report on a circadian oscillator in the cerebellum that is sensitive to feeding cues. Mice received intracerebroventricular injections of 0.12, 0.25, or 0.50 µg of OX7-SAP

The cerebellum harbors a circadian oscillator involved in food anticipation. Read More »

Enrichment of xenograft-competent genetically modified pig cells using a targeted toxin, isolectin BS-I-B4 conjugate.

Kristen Hartman

Akasaka E, Watanabe S, Himaki T, Ohtsuka M, Yoshida M, Miyoshi K, Sato M (2010) Enrichment of xenograft-competent genetically modified pig cells using a targeted toxin, isolectin BS-I-B4 conjugate. Xenotransplantation 17:81-89. doi: 10.1111/j.1399-3089.2010.00568.x Summary: Genetically modified pigs lacking the gala1-3gal epitope may be suitable for production of organs that could be transplanted to humans. The

Enrichment of xenograft-competent genetically modified pig cells using a targeted toxin, isolectin BS-I-B4 conjugate. Read More »

Targeting Tools: Leptin-SAP

News Tools / Kristen Hartman

Leptin-SAP (Cat. #IT-47) is a conjugate between recombinant mouse leptin and saporin. Leptin is a 16 kDa protein hormone that activates leptin receptors, and plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. Leptin inhibits the activity of neurons that contain neuropeptide Y (NPY) and agouti-related peptide (AgRP), and

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How long does it take to kill the target cell?

Kristen Hartman

Q: Saporin (Cat. #PR-01) has been shown to enzymatically inhibit the function of the ribosome, which follows that protein synthesis is then inhibited. Inhibition of protein synthesis brings about “cell death” to my knowledge. To detect “cell death” usually does not take a longer time to detect than “growth inhibition,” I suppose. So what I

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Are Hum-ZAP and Rat-ZAP bivalent?

Kristen Hartman

Q: Concerning Hum-ZAP (Cat. #IT-22) and Rat-ZAP (Cat. #IT-26), are they monovalent or bivalent to their target immunoglobulins? A: The secondary conjugates Hum-ZAP and Rat-ZAP are, in fact, bivalent and so do have the theoretical possibility of causing internalization when the primary would not – a false positive. In fact, we have never heard of

Are Hum-ZAP and Rat-ZAP bivalent? Read More »

Lot-to-Lot Variation

Kristen Hartman

Q: We have a question about two 192-IgG-SAP (Cat. #IT-01) lots. According to your data sheet there is an approximate 4-fold difference in ED50 between your new lot and old lot. We also observed a clear difference in behavior between animals dosed with the new batch and the old one. It is thus obvious that

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Anti-Melanopsin Protocol

Kristen Hartman

Q: We’re interested in trying out your melanopsin antibody (Cat. #AB-N38) using immunohistochemistry in mouse retina. Do you have a recommended protocol? A: This protocol has been utilized successfully with anti-melanopsin. Panda S. et al. 2002. Melanopsin (Opn4) requirement for normal light- induced circadian phase shifting. Science 298(5601):2213-2216. Related: Anti-Melanopsin (Cat. #AB-N38), Anti-Melanopsin, affinity-purified (Cat.

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Featured Article: Deletion of NPY/AGRP and POMC neurons in the arcuate nucleus by leptin-saporin produces hyperphagia, obesity and changes in diurnal feeding patterns in rats

Kristen Hartman

Li AJ, Wang Q, Dinh TT, Ritter S (2010) Featured Article: Deletion of NPY/AGRP and POMC neurons in the arcuate nucleus by leptin-saporin produces hyperphagia, obesity and changes in diurnal feeding patterns in rats. Targeting Trends 11(1) Related Products: Leptin-SAP (Cat. #IT-47), Blank-SAP (Cat. #IT-21) Read the featured article in Targeting Trends. See Also: Li

Featured Article: Deletion of NPY/AGRP and POMC neurons in the arcuate nucleus by leptin-saporin produces hyperphagia, obesity and changes in diurnal feeding patterns in rats Read More »

Author name: Kristen Hartman