New Secondary Conjugates – Fab-ZAPs – Use Monovalent Secondary Antibodies Linked to Saporin For the past decade scientists have extensively used secondary conjugates (e.g. Mab-ZAP; Cat. #IT-04, and Hum-ZAP; Cat. #IT-22) to make their own targeted toxins for in vitro use. The ability to combine: 1) a primary antibody to a cell-surface marker with 2) […]
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Anti-6 His-SAP (Cat. #IT-52) Composed of an antibody to 6 His conjugated to saporin, this secondary conjugate should be used as a diagnostic tool for testing your primary, 6 His-tagged proteins for specific cell surface epitope binding and internalization. The 6 His tag is widely used because of its affinity to bind nickel or cobalt
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Anti-GFP-SAP (Cat. #IT-53) The green fluorescent protein (GFP) gene can be introduced and maintained in the genome through breeding, injection with a viral vector, or cell transformation. Whether your cells express a surface protein with a GFP-coded region, or you have a protein that targets specific cells and contains a GFP tag, Anti-GFP-ZAP can be
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Dailey MJ, Bartness TJ (2010) Arcuate nucleus destruction does not block food deprivation-induced increases in food foraging and hoarding. Brain Res 1323:94-108. doi: 10.1016/j.brainres.2010.01.078 Summary: While some aspects of food intake are understood, mechanisms controlling hoarding of food have not been identified. This work investigates the role of NPY in the arcuate nucleus (Arc) in
Gulino R, Perciavalle V, Gulisano M (2010) Expression of cell fate determinants and plastic changes after neurotoxic lesion of adult mice spinal cord by cholera toxin-B saporin. Eur J Neurosci 31(8):1423-1434. doi: 10.1111/j.1460-9568.2010.07170.x Summary: Sonic hedgehog, Notch-1, and Numb are proteins known to be involved in the function of stem cells. Understanding of how they
Ariizumi K, Akiyoshi H, Chung JS, Tomiharu M, Cruz Jr PD (2010) Featured Article: Depletion of syndecan-4+ T lymphocytes by saporin-conjugated DC-HIL alleviates T cell-mediated imflammatory disease. Targeting Trends 11(2) Related Products: Streptavidin-ZAP (Cat. #IT-27) Read the featured article in Targeting Trends. See Also: Akiyoshi H et al. Depleting Syndecan-4+ T Lymphocytes Using Toxin-Bearing Dendritic
Contributed by Kiyoshi Ariizumi, Hideo Akiyoshi, Jin-Sung Chung, Mizuki Tomiharu, Ponciano D. Cruz Jr. Department of Dermatology, The University of Texas Southwestern Medical Center and Dermatology Section (Medical Service), Dallas Veterans Affairs Medical Center, Dallas, TX T lymphocyte activation is regulated by stimulatory and inhibitory signals transduced by binding of T cell receptors to corresponding
Larsson M, Broman J (2011) Synaptic plasticity and pain: role of ionotropic glutamate receptors. Neuroscientist 17(3):256-73. doi: 10.1177/1073858409349913 Summary: This review discusses the role of glutaminergic sensory synapses in pain hypersensitivity caused by tissue or nerve injury. The focus is on the roles of ionotrophic glutamate receptors, and how they are involved in dorsal horn
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Akiyoshi H, Chung JS, Tomihari M, Cruz PD, Jr., Ariizumi K (2010) Depleting syndecan-4+ T lymphocytes using toxin-bearing dendritic cell-associated heparan sulfate proteoglycan-dependent integrin ligand: A new opportunity for treating activated T cell-driven disease. J Immunol 184:3554-3561. doi: 10.4049/jimmunol.0903250 Summary: The dendritic cell-associated heparin sulfate proteoglycan-dependent integrin ligand (DC-HIL) exclusively associates with syndecan-4 (SD-4), which
De Bartolo P, Cutuli D, Ricceri L, Gelfo F, Foti F, Laricchiuta D, Scattoni ML, Calamandrei G, Petrosini L (2010) Does age matter? Behavioral and neuro-anatomical effects of neonatal and adult basal forebrain cholinergic lesions. J Alzheimers Dis 20:207-227. doi: 10.3233/JAD-2010-1355 PMID: 20164586 Summary: The authors characterized the differences caused by age on the effect