Q: Saporin (Cat. #PR-01) has been shown to enzymatically inhibit the function of the ribosome, which follows that protein synthesis is then inhibited. Inhibition of protein synthesis brings about “cell death” to my knowledge. To detect “cell death” usually does not take a longer time to detect than “growth inhibition,” I suppose. So what I […]
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Q: Concerning Hum-ZAP (Cat. #IT-22) and Rat-ZAP (Cat. #IT-26), are they monovalent or bivalent to their target immunoglobulins? A: The secondary conjugates Hum-ZAP and Rat-ZAP are, in fact, bivalent and so do have the theoretical possibility of causing internalization when the primary would not – a false positive. In fact, we have never heard of
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Q: We have a question about two 192-IgG-SAP (Cat. #IT-01) lots. According to your data sheet there is an approximate 4-fold difference in ED50 between your new lot and old lot. We also observed a clear difference in behavior between animals dosed with the new batch and the old one. It is thus obvious that
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Q: We’re interested in trying out your melanopsin antibody (Cat. #AB-N38) using immunohistochemistry in mouse retina. Do you have a recommended protocol? A: This protocol has been utilized successfully with anti-melanopsin. Panda S. et al. 2002. Melanopsin (Opn4) requirement for normal light- induced circadian phase shifting. Science 298(5601):2213-2216. Related: Anti-Melanopsin (Cat. #AB-N38), Anti-Melanopsin, affinity-purified (Cat.
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Li AJ, Wang Q, Dinh TT, Ritter S (2010) Featured Article: Deletion of NPY/AGRP and POMC neurons in the arcuate nucleus by leptin-saporin produces hyperphagia, obesity and changes in diurnal feeding patterns in rats. Targeting Trends 11(1) Related Products: Leptin-SAP (Cat. #IT-47), Blank-SAP (Cat. #IT-21) Read the featured article in Targeting Trends. See Also: Li
Thomsen MS, Hay-Schmidt A, Hansen HH, Mikkelsen JD (2010) Distinct neural pathways mediate alpha7 nicotinic acetylcholine receptor-dependent activation of the forebrain. Cereb Cortex 20(9):2092-2102. doi: 10.1093/cercor/bhp283 Summary: a7 nicotinic acetylcholine receptor (nAChR) agonists are potential treatments for some aspect of schizophrenia. The authors examine whether cholinergic neurons in the horizontal limb of the diagonal band
Contributed by Ai-Jun Li, Qing Wang, Thu T. Dinh and Sue Ritter Programs in Neuroscience, Washington State University, Pullman, Washington 99164-6520, USA Leptin is a fat tissue-derived hormone with widespread actions in brain and peripheral tissues. Leptin’s actions are mediated by the long isoform of the leptin receptor, OB-Rb, which has a lengthy intracellular region
Read the 2009 Newsletters and recent scientific references 2009, issue 1: Selective deletion of CD8+ T cells by saporin-coupled MHC class I tetramers / by Hess PR, Buntzman AS, Murray SL, Young EF, Frelinger JA / featuring Streptavidin-ZAP (Cat. #IT-27), FITC-labeled SAP antibody (Cat. #FL-02) SFN Poster of the Year Targeting Talk: IgM Primary Antibody Assay 2009, issue 2:
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Gibbs RB (2010) Estrogen therapy and cognition: a review of the cholinergic hypothesis. Endocr Rev 31(2):224-253. doi: 10.1210/er.2009-0036 Summary: This review discusses estrogen therapy for use in postmenopausal women. In this context the issues revolve around benefits vs. harm of such therapy on the brain and cognitive impairment associated with aging and Alzheimer’s disease. Use
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Robertson RT, Baratta J, Yu J, LaFerla FM (2009) Amyloid-beta expression in retrosplenial cortex of triple transgenic mice: relationship to cholinergic axonal afferents from medial septum. Neuroscience 164:1334-1346. doi: 10.1016/j.neuroscience.2009.09.024 Summary: In this work the authors developed a model to examine the relationship between afferent projections and the formation of amyloid-beta (Aβ) deposits. Mice received