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Summary: The antibody CD105 (1:1,000) was incubated with Mab-ZAP, to allow binding and formation of a CD105 antibody-saporin complex, which was added to explants or to a human cerebral microvessel endothelial cell line as control. CD105 antibody was also incubated with a Goat-IgG-SAP for control that does not bind CD105. The conjugates were injected into the explant under a dissecting microscope after gently incising the explant surface for better access.