Hovinga KE, Shimizu F, Wang R, Panagiotakos G, Van Der Heijden M, Moayedpardazi H, Correia AS, Soulet D, Major T, Menon J, Tabar V (2010) Inhibition of notch signaling in glioblastoma targets cancer stem cells via an endothelial cell intermediate. Stem Cells 28:1019-1029. doi: 10.1002/stem.429
Summary: The antibody CD105 (1:1,000) was incubated with Mab-ZAP, to allow binding and formation of a CD105 antibody-saporin complex, which was added to explants or to a human cerebral microvessel endothelial cell line as control. CD105 antibody was also incubated with a Goat-IgG-SAP for control that does not bind CD105. The conjugates were injected into the explant under a dissecting microscope after gently incising the explant surface for better access.
Related Products: Mab-ZAP (Cat. #IT-04), Goat IgG-SAP (Cat. #IT-19)