Tag Archives: 15q4

Targeting Trends Newsletter 15q4

Below are links to view the quarterly newsletter Targeting Trends. You can also download the full PDF here. If you would like to be added to the mailing list, please complete the information on our contact page. Anti-ChAT-SAP is highlighted this quarter in the cover article “A specific immunotoxin elucidates a causal role of striatal cholinergic […]

Targeting Tools: MonoBiotin-ZAP

The conjugation specialists at Advanced Targeting Systems are proud to announce a new addition to the catalog: MonoBiotin-ZAP Cat. #BT-ZAP Researchers now have more freedom in selecting conjugation strategies. Users have taken advantage of the ability to use biotinylated targeting agents with Streptavidin-ZAP (Cat. #IT-27) a chemical conjugate between saporin and streptavidin. Streptavidin-ZAP converts biotinylated […]

Targeting Tools: Anti-ChAT-SAP

Choline acetyltransferase (ChAT) catalyzes the synthesis of the neurotransmitter acetylcholine (ACh) from choline and acetyl-CoA in cholinergic neurons. ChAT serves as a specific marker for cholinergic neurons in both peripheral and central nervous systems. Evidence shows that ChAT exists in two forms inside cholinergic nerve terminals, a soluble hydrophilic form and the membrane-associated amphiphilic form.[1-2] […]

Targeting Talk: Retrograde Transport

Q: I’m trying to find out if enough Anti-DBH-SAP will be retrogradely transported and taken up by non targeted sympathetic neurons by bulk fluid-phase endocytosis. Does saporin become degraded after it kills the neuron or does it enter the extracellular matrix? A: It is very unlikely that a targeted toxin such as Anti-DBH-SAP is freed […]

Targeting Talk: Suicide Transport and Immunolesioning Book

Q: Hello, I have used your Anti-DBH-SAP (Cat. #IT-03) conjugate, and I’m having a hard time finding this citation: R.G. Wiley, D.A. Lappi. Suicide Transport and Immunolesioning. Molecular Biology Intelligence Unit, R.G. Landes Co, Austin, TX (1994). Do you know where I could find a copy? A: This book is available in many university libraries […]

Targeting Topics 15q4

Alterations in the rostral ventromedial medulla after the selective ablation of mu-opioid receptor expressing neurons. Harasawa I, Johansen JP, Fields HL, Porreca F, Meng ID. Pain 157(1):166-173, 2016. The rostral ventromedial medulla (RVM) has both excitatory and inhibitory control over nociceptive neurons in the medullary dorsal horn and spinal cord. Previous work has demonstrated that […]

Cover Article: Anti-ChAT-SAP elucidates a causal role in behavioral flexibility

A specific immunotoxin elucidates a causal role of striatal cholinergic system in behavioral flexibility by Sho Aoki and Jeffery R. Wickens Neurobiology Research Unit, Okinawa Institute of Science and Technology, 1919-1 Tancha, Onna, Kunigami, Okinawa 904-0495, Japan Behavioral flexibility is broadly defined as the ability to change behavioral strategy, according to a change of governing […]