Below are links to view the quarterly newsletter Targeting Trends. If you would like to be added to the mailing list, please complete the information on our contact page. Newsletter Highlights FENS-Barcelona; Spring Brain Conference (page 2) LD50 of saporin immunotoxins in mice; Antagonists vs. Targeted Toxins (page 5) Teaser Winners (page 5) Mac-1-SAP; Anti-GST; Anti-SERT-SAP …
The Mac-1-SAP targeted toxins recognize Mac-1-positive (CD11b) cells in mouse/human (Cat. #IT-06) or in rat (Cat. #IT-33). The toxin is excellent for removing contaminating macrophages from primary cultures. Elimination of macrophages in vivo is useful to determine their role(s) in autoimmune diseases and in degenerative diseases such as Alzheimer’s. Both toxins are extremely potent, with …
The Glutathione S-Transferase (GST) family of enzymes contains cytosolic, mitochondrial, and microsomal proteins that are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. GST catalyzes the conjugation of reduced glutathione which is useful in the detoxification of endogenous compounds such as peroxidized lipids, as well as the metabolism of xenobiotics. …
Anti-SERT-SAP (Cat. #IT-23) utilizes a monoclonal antibody to the fourth extracellular domain of the serotonin re-uptake transporter (SERT). SERT is the major determinant of serotonin inactivation following release at synapses, is the site of action for many tricyclic antidepressants and the SSRIs (serotonin-selective reuptake inhibitors), and is also targeted by a number of psychostimulants including …
What is the LD50 of saporin in mice? Do you have references for this? Thank you for your question. It is very helpful to have this information to calculate the appropriate dose for systemic administration. According to the work of Thorpe et al. (J Natl Cancer Inst. 75(1):151-159, 1985), saporin alone has an acute LD50, …
Recently, I attended a talk where the speaker said that a targeted toxin was able to work when an antagonist did not. Can you explain how your technology is different? Certainly. It is a very interesting question and one that helps explain the Targeting Technology quite well. An antagonist is used to block a receptor …
C1 neurons excite locus coeruleus and A5 noradrenergic neurons along with sympathetic outflow in rats. Abbott SB, Kanbar R, Bochorishvili G, Coates MB, Stornetta RL, Guyenet PG. J Physiol 590(12):2897-2915, 2012. C1 neurons are known to activate sympathetic tone and stimulate the hypothalamic-pituitary-adrenal axis. C1 activation is also reported to inhibit locus coeruleus (LC) neurons. …
Contributed by Lique M. Coolen, University of Michigan Introduction from Dr. Lappi: An article by Lique Coolen’s group[5] was the 1000th article in PubMed in response to the search word ‘saporin.’ Dr. Coolen has graciously agreed to describe the latest in her fascinating work. Male sexual behavior consists of many different aspects, with ejaculation being …