12q2

Targeting Trends 2012 Newsletter

Read the 2012 Newsletters 2012, issue 1:  Motivation’s modulation of attention through the mesolimbic-corticopetal cholinergic circuitry / by M St. Peters, M Sarter / featuring 192-IgG-SAP (Cat. #IT-01), Mouse IgG-SAP (Cat. #IT-18) SFN Poster of the Year Targeting Talk: Anti-NGFr blocking antibody, Staining with FITC-labeled Anti-Saporin Targeting Tools: Antibody to Metabotropic Glutamate Receptor 2, Tag-Targeted Toxins …

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Targeting Tools: Anti-RFP

Red fluorescent protein (RFP) is derived from Discosoma sea anemone. This antibody (Anti-RFP, Cat. #AB-332) recognizes native and denatured forms of RFP. The antigen for this antibody is present in all of these fluorescent molecules: RFP, tag-RFP, turbo-RFP, DeRed, mCherry, and mOrange. The epitope for the antibody is part of the RFP N-terminal sequence. RFP …

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Targeting Tools: NK3-SAP

An analog of the Neurokinin B peptide, [MePhe7]-NKB, is a selective agonist of the NK3 receptor. Conjugation of [MePhe7]-NKB to saporin (NK3-SAP) allows for the specific elimination of a subpopulation of neurons expressing kisspeptin, neurokinin B, and dynorphin (KNDy neurons) through their co-expression of the NK3 receptor. Although NK3 receptor expressing neurons reside in several …

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Targeting Tools: Chick-ZAP

Chick-ZAP is the most recent in our line of Secondary Conjugates. It is a chemical conjugate of affinity-purified rabbit anti-chicken IgY and the ribosome-inactivating protein, saporin. The conjugate will target and eliminate cells that recognize your primary antibody. This reagent can be utilized for screening chicken IgY antibodies for internalization and/or their suitability to make …

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Targeting Topics 12q2

IB4-saporin attenuates acute and eliminates chronic muscle pain in the rat. Alvarez P, Gear RW, Green PG, Levine JD. Exp Neurol 233(2):859-865, 2012. In order to clarify the roles of isolectin B4-positive and IB4-negative nociceptors in inflammatory and ergonomic muscle pain, the authors administered 3.2 µg of IB4-SAP (Cat. #IT-10) into the intrathecal space of …

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Cover Article: Use of a novel saporin conjugate (NK3-SAP) to study the function of neurokinin 3 receptor (NK3r)-expressing kisspeptin/neurokinin B/dynorphin (KNDy) neurons in the rat arcuate nucleus

Contributed by Naomi E. Rance, Melinda. A. Mittelman-Smith and Sally J. Krajewski-Hall Department of Pathology, University of Arizona College of Medicine This year, our two posters shared the ATS Poster of the Year Award at the Society for Neuroscience Meeting. These posters documented the use of NK3-SAP, a custom conjugate of saporin (Cat. #IT-63) with …

Cover Article: Use of a novel saporin conjugate (NK3-SAP) to study the function of neurokinin 3 receptor (NK3r)-expressing kisspeptin/neurokinin B/dynorphin (KNDy) neurons in the rat arcuate nucleus Read More »