10q2

Targeting Trends 2010 Newsletter

2010, issue 1: Deletion of NPY/AGRP and POMC Neurons in the Arcuate Nucleus by Leptin-Saporin Produces Hyperphagia, Obesity and Changes in Diurnal Feeding Patterns in Rats / by Li AJ, Wang Q, Dinh TT, Ritter S / featuring Leptin-SAP (Cat. #IT-47) and Blank-SAP (Cat. #IT-21) SFN Poster of the Year Targeting Talk: Product Questions Targeting Tools: Leptin-SAP (Cat. #IT-47) …

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Targeting Tools: Fab-ZAPs

New Secondary Conjugates – Fab-ZAPs – Use Monovalent Secondary Antibodies Linked to Saporin For the past decade scientists have extensively used secondary conjugates (e.g. Mab-ZAP; Cat. #IT-04, and Hum-ZAP; Cat. #IT-22) to make their own targeted toxins for in vitro use. The ability to combine: 1) a primary antibody to a cell-surface marker with 2) …

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Targeting Tools: Anti-6 His-ZAP

Anti-6 His-ZAP (Cat. #IT-52) Composed of an antibody to 6 His conjugated to saporin, this secondary conjugate should be used as a diagnostic tool for testing your primary, 6 His-tagged proteins for specific cell surface epitope binding and internalization. The 6 His tag is widely used because of its affinity to bind nickel or cobalt …

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Targeting Tools: Anti-GFP-ZAP

Anti-GFP-ZAP (Cat. #IT-53) The green fluorescent protein (GFP) gene can be introduced and maintained in the genome through breeding, injection with a viral vector, or cell transformation. Whether your cells express a surface protein with a GFP-coded region, or you have a protein that targets specific cells and contains a GFP tag, Anti-GFP-ZAP can be …

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Targeting Topics 10q2

Enrichment of xenograft-competent genetically modified pig cells using a targeted toxin, isolectin BS-I-B4 conjugate Akasaka E, Watanabe S, Himaki T, Ohtsuka M, Yoshida M, Miyoshi K, Sato M Xenotransplantation 17(1):81-89, 2010. Genetically-modified pigs lacking the gala1-3gal epitope may be suitable for production of organs that could be transplanted to humans. The ability to select for …

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Cover Article: Depletion of syndecan-4+ T lymphocytes by saporin-conjugated DC-HIL alleviates T cell-mediated imflammatory disease

Contributed by Kiyoshi Ariizumi, Hideo Akiyoshi, Jin-Sung Chung, Mizuki Tomiharu, Ponciano D. Cruz Jr. Department of Dermatology, The University of Texas Southwestern Medical Center and Dermatology Section (Medical Service), Dallas Veterans Affairs Medical Center, Dallas, TX T lymphocyte activation is regulated by stimulatory and inhibitory signals transduced by binding of T cell receptors to corresponding …

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