Chick-ZAP uses your chicken IgY primary antibody to target and eliminate cells that recognize your primary antibody. This secondary conjugate uses the secondary antibody (affinity-purified rabbit anti-chicken IgY) to “piggyback” onto YOUR chicken primary antibody. Chick-ZAP be utilized for screening chicken IgY antibodies for internalization and/or their suitability to make potent immunotoxins. When the in vitro results confirm the desired specificity, it is recommended that you order a custom conjugation of your antibody to saporin.
Chick-ZAP is a chemical conjugate of affinity-purified rabbit anti-chicken IgY and the ribosome-inactivating protein, saporin. It uses your chicken IgY primary antibody to target and eliminate cells. This secondary conjugate is used to evaluate the potential of a primary antibody to internalize.
Chick-ZAP is available individually (Cat. #IT-62) or as a kit (Cat. #KIT-62) which includes Chick-ZAP, Saporin (Cat. #PR-01), Rabbit IgG-SAP (Cat. #IT-35) and reagents for developing a cytotoxicity assay.