SP-CTA [IT-39, KIT-39]

a tool for activating cells that express substance P receptor (NK-1); targeted via Substance P, activated via the catalytic A subunit of cholera toxin

SKU: IT-39 Category: Quantity: Individual 25 ug, Individual 100 ug, Individual 250 ug, Individual 1 mg, Kit w/controls 25 ug, Kit w/controls 100 ug, Kit w/controls 250 ug | Conjugate: CTA | Usage: stimulates cells |

SP-CTA is an exciting tool for use in the research of neurokinin (NK-1) receptor-expressing cells of the central nervous system. It can be used very effectively in vivo for increasing sensitization of these neuronal cells. Selectively targeting the NK-1R-expressing cells with the substance P moiety allows the researcher to stimulate only the cells of interest by amplifying their cAMP production with the CTA, without altering the neighboring cells. This effect lasts for a few days, and gives the researcher an opportunity to study behaviors such as those related to the perception of pain or the control of breathing.

SP-CTA is a chemical conjugate of the neuropeptide substance P and the catalytic A subunit of cholera toxin. It stimulates cells expressing the substance P receptor.

SP-CTA is available individually (Cat. #IT-39) or as a kit (Cat. #KIT-39) which includes SP-CTA and Blank-CTA (Cat. #IT-61).

keywords: cholera toxin subunit A, CT, CTA, CTB, catalytic A subunit, substance P, SP, neurokinin receptor, NK-1 receptor, NK-1R, NK1R, central nervous system, CNS, sensitization, cAMP, stimulation

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View Data Sheet

Central sensitization in the trigeminal nucleus caudalis produced by a conjugate of substance P and the A subunit of cholera toxin.

Caudle R, King C, Nolan T, Suckow S, Vierck C, Neubert J (2010) Central sensitization in the trigeminal nucleus caudalis produced by a conjugate of substance P and the A subunit of cholera toxin. J Pain 11:838-846. doi: 10.1016/j.jpain.2010.05.007

Objective: To understand central sensitization.

Summary: Findings suggest that central sensitization leads to activation of an endogenous opioid system. Intracisternal administration of SP-CTA is a useful model for studying central sensitization as a disease process without having to induce a peripheral injury.

Usage: SP-CTA was diluted in PBS and injected in a volume of 10 μl (rats) or 1 μl (mice) over the course of three minutes.

Related Products: SP-CTA (Cat. #IT-39)

Sleep-inducing effect of substance P-cholera toxin A subunit in mice.

Zielinski M, Gerashchenko D (2017) Sleep-inducing effect of substance P-cholera toxin A subunit in mice. Neurosci Lett 659:44-47.. doi: 10.1016/j.neulet.2017.08.066

Objective: To determine the effects of selectively activating SP-expressing brain cells on sleep regulation in mice.

Summary: ICV administration of SP-CTA produces increased amounts of NREM sleep but induces sleep fragmentation.

Usage: 1 mcg/mcl icv.

Related Products: SP-CTA (Cat. #IT-39)

Sensitization of spinal cord nociceptive neurons with a conjugate of substance P and cholera toxin.

Caudle RM (2007) Sensitization of spinal cord nociceptive neurons with a conjugate of substance P and cholera toxin. BMC Neuroscience 8:30. doi: 10.1186/1471-2202-8-30

Related Products: SP-CTA (Cat. #IT-39), Neurotensin-CTA (Cat. #IT-60)

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CTA Products:

SP-CTA (Cat. #IT-39)

Neurotensin-CTA (Cat. #IT-60)

Blank-CTA (Cat. #IT-61)

Cholera Toxin subunit A Rabbit Polyclonal (Cat. #AB-43)

Inducing Central Sensitization with a Substance P/ Cholera Toxin Conjugate

Targeting Tools: Stimulate Instead of Eliminate

Selective Activation in Neuronal Populations

Cytotoxicity Assay for Targeted Toxins in vitro

Concentration Calculation Explained: Convert molarity to mg/ml and mg/ml to molarity

Preparing and Interpreting Cytotoxicity Data in vitro


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