2019 Targeting Trends Review

Wang Z, Wu J-L, Zhong F, Liu Y, Yu Y-Q, Sun J-J, Wang S, Li H, Zhou X-F, Hu Z-L, Dai R-P (2019) Upregulation of proBDNF in the mesenteric lymph nodes in septic mice. Neurotox Res 36(3):540-550. doi: 10.1007/s12640-019-00081-3 PMID: 31278527

Objective: To characterize the expression pattern of proBDNF in mesenteric lymph nodes (MLNs) after lipopolysaccharide (LPS) stimulation, and to investigate the association of pathogenesis of sepsis.

Summary: This study provided direct evidence that sepsis promoted the proliferation of CD3+, CD4+, and CD8+ T cells, but inhibits CD19+ B cells in the MLNs. Also, proBDNF was upregulated during the sepsis, which was mainly localized on CD3+ and CD4+ T cells in MLNs. The upregulation of proBDNF signaling and cytokines activation in the MLNs may be involved in the disease progress of sepsis.

Usage: western blot (1:2000)

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Nizari S, Carare RO, Romero IA, Hawkes CA (2019) 3D reconstruction of the neurovascular unit reveals differential loss of cholinergic innervation in the cortex and hippocampus of the adult mouse brain. Front Aging Neurosci 11:172. doi: 10.3389/fnagi.2019.00172

Objective: To further characterize the effect of the loss of cholinergic innervation on the NVU (neurovascular unit) in Alzheimer’s Disease.

Summary: Significantly less ChAT staining was detected in the medial septum of saporin-treated mice at 45 days post-surgery. This was accompanied by a significant decrease in cholinergic nerve fiber density in the hippocampus and the cortex. As expected, p75 NTR-negative neurons in the striatum were not affected by mu p75-SAP treatment.

Usage: In this study, the mu-p75-SAP was used to induce death of basal forebrain cholinergic neurons and their fiber projections. mu p75-SAP 0.5 µL (0.596 µg/µL) or 0.9% saline (n = 19) was injected into each ventricle.

Related Products: mu p75-SAP (Cat. #IT-16)

Hernández-Melesio MA, Alcaraz-Zubeldia M, Jiménez-Capdeville ME, Martínez-Lazcano JC, Santoyo-Pérez ME, Quevedo-Corona L, Gerónimo-Olvera C, Sánchez-Mendoza A, Ríos C, Pérez-Severiano F (2019) Nitric oxide donor molsidomine promotes retrieval of object recognition memory in a model of cognitive deficit induced by 192 IgG-saporin. Behav Brain Res 366:108-117. doi: 10.1016/j.bbr.2019.03.031

Objective: To analyze the potential of a NO donor (molsidomine, MOLS) to prevent the recognition memory deficits resulting from the septal cholinergic denervation by 192-IgG-SAP in rats.

Summary: Results showed that 192-IgG-SAP reduced the immunoreactivity of cholinergic septal neurons (41%), compared with PBS-receiving control rats (p < 0.05).

Usage: The injection reached the medial septum (MS) structure with 192-IgG-SAP diluted in PBS solution (0.22 μg in 1μl) or PBS as control, both at 0.25 μl/min, allowing diffusion for 3 min, AP+0.6, L 0.0, V−7.0 from Bregma.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Xu T, Zhou Z, Liu N, Deng C, Huang G, Zhou F, Liu X, Wang X (2019) Disrupted compensatory response mediated by Wolfram syndrome 1 protein and corticotrophin-releasing hormone family peptides in early-onset intrahepatic cholestasis pregnancy. Placenta 83:63-71. doi: 10.1016/j.placenta.2019.06.378 PMID: 31477210

Usage: Western (1:1000)

Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)

Weiner GA, Shah SH, Angelopoulos CM, Bartakova AB, Pulido RS, Murphy A, Nudleman E, Daneman R, Goldberg JL (2019) Cholinergic neural activity directs retinal layer-specific angiogenesis and blood retinal barrier formation. Nat Commun 10(1):2477. doi: 10.1038/s41467-019-10219-8

Objective: To determine which neurons are responsible for angiogenesis and blood retinal barrier formation.

Summary: Anti-ChAT-SAP reduces SAC (starburst amacrine cell) number and inhibits deep-layer angiogenesis.

Usage: Anti-ChAT-SAP or control Rabbit-IgG-SAP were injected intravitreally at P3 and P11 (0.12 mg/mL in PBS).

Related Products: Anti-ChAT-SAP (Cat. #IT-42), Rabbit IgG-SAP (Cat. #IT-35)

Beitia M, Solano-Iturri JD, Errarte P, Sanz B, Perez I, Etxezarraga MC, Loizate A, Asumendi A, Larrinaga G (2019) Altered expression of renin-angiotensin system receptors throughout colorectal adenoma-adenocarcinoma sequence. Int J Med Sci 16(6):813-821. doi: 10.7150/ijms.32599 PMID: 31337954

Usage: immunohistochemistry (1:100)

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Soares DdS, Pinto GH, Lopes A, Caetano DSL, Nascimento TG, Andrades ME, Clausell N, Rohde LEP, Leitão SAT, Biolo A (2019) Cardiac hypertrophy in mice submitted to a swimming protocol: Influence of training volume and intensity on myocardial renin-angiotensin system. Am J Physiol Regul Integr Comp Physiol 316(6):R776-R782. doi: 10.1152/ajpregu.00205.2018 PMID: 31042418

Objective: To analyze the cardiac hypertrophy phenotype and the classical and alternative RAS axes in the myocardium of mice submitted to swimming exercises of varying volume and intensity for the development of cardiac hypertrophy.

Summary: Increasing exercise volume and intensity did not induce a pathological hypertrophy phenotype, but instead improved the oxidative capacity, and this process might have the participation of the RAS alternative axis.

Usage: Incubation at 4°C with anti-AT1R diluted in TTBS buffer with 5% non-fat 220 milk 1:800).

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

de Miguel E, Vekovischeva O, Elsilä LV, Panhelainen A, Kankuri E, Aitta-aho T, Korpi ER (2019) Conditioned aversion and neuroplasticity induced by a superagonist of extrasynaptic gabaa receptors: Correlation with activation of the oval BNST neurons and CRF mechanisms. Front Mol Neurosci 12:130. doi: 10.3389/fnmol.2019.00130 PMID: 31178693

Usage: immunohistochemistry (1:250)

Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)

Liang YJ, Feng SY, Qi YP, Li K, Jin ZR, Jing HB, Liu LY, Cai J, Xing GG, Fu KY. (2019) Contribution of microglial reaction to increased nociceptive responses in high-fat-diet (HFD)-induced obesity in male mice. Brain Behav Immun 80:777-792. doi: 10.1016/j.bbi.2019.05.026

Related Products: Mac-1-SAP mouse/human (Cat. #IT-06)

Bernabe C, Caliman I, de Abreu A, Dustrude E, Molosh A, Shekhar A, Johnson P (2019) S38. Dissecting the functional heterogeneity of serotonergic systems that regulate fear and panic. Biol Psychiatry 85(10):S311. doi: 10.1016/j.biopsych.2019.03.789

Objective: To elucidate the role of these serotonergic networks on learned fear and innate panic responses.

Summary: LED excitation or lesioning of PeF projecting 5-HT system respectively attenuated and enhanced panic-associated escape/flight behaviors and cardioexcitation following a 7.5 and 20% CO2 challenge.

Usage: Anti-SERT-SAP was injected into the BLA or PeF to lesion these 5-HT pathways.

Related Products: Anti-SERT-SAP (Cat. #IT-23)