2002 Targeting Trends Review

Cassel J, Gaurivaud M, Lazarus C, Bertrand F, Galani R, Jeltsch H (2002) Grafts of fetal septal cells after cholinergic immunotoxic denervation of the hippocampus: a functional dissociation between dorsal and ventral implantation sites. Neuroscience 113(4):871-882. doi: 10.1016/s0306-4522(02)00226-9

Summary: The authors lesioned rats with intraseptal infusions of 0.8 µg 192-Saporin (Cat. #IT-01), then implanted fetal cells in either the dorsal or ventral hippocampus. Only grafts into the dorsal hippocampus counteracted the effect of cholinergic lesions on spatial working memory performance.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Truitt WA, Coolen LM (2002) Identification of a potential ejaculation generator in the spinal cord. Science 297(5586):1566-1569. doi: 10.1126/science.1073885

Summary: The authors lesioned a specific population of rat spinothalamic neurons using 6-8 injections of 4 ng SSP-SAP (Cat. #IT-11). Whereas the treated rats exhibited no change in sexual behavior such as mounts and intromissions, ejaculatory behavior was completely abolished. The data suggest that this population of neurons may function as an ejaculation generator in the spinal cord.

Related Products: SSP-SAP (Cat. #IT-11)

Simons CT, Gogineni AG, Iodi Carstens M, Carstens E (2002) Reduced aversion to oral capsaicin following neurotoxic destruction of superficial medullary neurons expressing NK-1 receptors. Brain Res 945:139-143. doi: 10.1016/s0006-8993(02)02913-x

Summary: Capsaicin-induced irritation of the dorsal anterior tongue is mediated by nociceptors expressing VR-1 receptors. The role of NK-1 receptor-expressing neurons during the ingestion of capsaicin was examined by injecting 20 µl of 2.27 µM SP-SAP (Cat. #IT-07) into the cisterna magna of rats. Lesioned rats consumed significantly more water containing high concentrations of capsaicin than control animals.

Related Products: SP-SAP (Cat. #IT-07)

Lehmann O, Jeltsch H, Lazarus C, Tritschler L, Bertrand F, Cassel JC (2002) Combined 192 IgG-saporin and 5,7-dihydroxytryptamine lesions in the male rat brain: A neurochemical and behavioral study. Pharmacol Biochem Behav 72(4):899-912. doi: 10.1016/s0091-3057(02)00752-9

Summary: Female rats treated with both 192-Saporin (Cat #IT-01) and 5,7-DHT exhibited working memory impairments that were not seen with either treatment alone. For this paper, male rats were treated with 5,7-DHT and 1 µg per ventricle of 192-Saporin. While each compound produced a unique combination of effects, cognitive performance was only affected by treatment with both agents. These data indicate that interaction between the cholinergic and serotonergic mechanisms plays a role in cognitive functions for both sexes.

Usage: Injections of 1 µg per ventricle of 192-Saporin (Cat. #IT-01).

Related Products: 192-IgG-SAP (Cat. #IT-01)

Read the featured article in Targeting Trends.

Johnson DA, Zambon NJ, Gibbs RB (2002) Selective lesion of cholinergic neurons in the medial septum by 192 IgG-saporin impairs learning in a delayed matching to position T-maze paradigm. Brain Res 943(1):132-141. doi: 10.1016/s0006-8993(02)02623-9

Summary: The authors investigated the effects of selective cholinergic depletion in the medial septum on a spatial memory (DMP) task. Direct infusion of 0.22 or 1.0 µg 192-Saporin (Cat. #IT-01) produced a near complete depletion of cholinesterase-positive neurons for either dose. The DMP task provides a sensitive behavioral assay for deficits in cholinergic projections.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Easton A, Ridley RM, Baker HF, Gaffan D (2002) Unilateral lesions of the cholinergic Basal forebrain and fornix in one hemisphere and inferior temporal cortex in the opposite hemisphere produce severe learning impairments in rhesus monkeys. Cereb Cortex 12(7):729-736. doi: 10.1093/cercor/12.7.729

Summary: The authors used a combination of basal forebrain lesioning using ME20.4-SAP (Cat. #IT-15) and surgery to isolate the inferior temporal cortex and medial temporal cortex from cholinergic afferents in rhesus monkeys. Testing of the treated animals demonstrated severe impairments in learning visual scenes and object-reward associations.

Related Products: ME20.4-SAP (Cat. #IT-15)

Burgess SE, Gardell LR, Ossipov MH, Malan Jr TP, Vanderah TW, Lai J, Porreca F (2002) Time-dependent descending facilitation from the rostral ventromedial medulla maintains, but does not initiate, neuropathic pain. J Neurosci 22(12):5129-5136. doi: 10.1523/JNEUROSCI.22-12-05129.2002

Summary: Various indications, such as declining afferent discharge over time, suggest that the mechanisms involved in persistent neuropathic pain are different than those that initiate the pain. The authors have previously shown that cells expressing the mu-opioid receptor are involved in the descending pain pathway. In this work, the authors lesioned the rostral ventromedial medulla (RVM) in rats using 1.5 pmol in 0.5 µl of dermorphin-SAP (Cat. #IT-12) administered to each side of the RVM. Measurements of pain-related behavior show that mu-opioid receptor-expressing cells in the RVM are involved in the maintenance of heightened sensitivity to stimuli seen in neuropathic pain.

Related Products: Dermorphin-SAP / MOR-SAP (Cat. #IT-12)

Galani R, Jeltsch H, Lehmann O, Bertrand F, Cassel JC (2002) Effects of 192 IgG-saporin on acetylcholinesterase histochemistry in male and female rats. Brain Res Bull 58(2):179-186. doi: 10.1016/s0361-9230(02)00775-x

Summary: Male rats were treated with estradiol, and 2-µg i.c.v. injections of 192-Saporin (Cat #IT-01).

Related Products: 192-IgG-SAP (Cat. #IT-01)

Fraley GS, Dinh TT, Ritter S (2002) Immunotoxic catecholamine lesions attenuate 2DG-induced increase of AGRP mRNA. Peptides 23(6):1093-1099. doi: 10.1016/s0196-9781(02)00044-x

Summary: The authors investigated mRNA levels of both agouti gene-related protein (AGRP) and neuropeptide Y (NPY) in rats after lesioning the PVH with anti-DBH-SAP (42 ng in 200 nl, Cat. #IT-03). The results show that the increase in AGRP mRNA levels due to 2DG administration was completely blocked.

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Bitner RS, Nikkel AL (2002) Alpha-7 nicotinic receptor expression by two distinct cell types in the dorsal raphe nucleus and locus coeruleus of rat. Brain Res 938:45-54. doi: 10.1016/s0006-8993(02)02485-x

Summary: Neuronal nicotinic acetylcholine receptors (nAChRs) are suspected to play a role in neurophysiological disorders such as schizophrenia, Alzheimer’s disease, and epilepsy. Whereas the molecular and cellular properties of these receptors have been well characterized, the role of nAChRs in the nervous system is as yet unclear. The authors injected rats intracerebroventricularly with 5 µg/5 µl of anti-DBH-SAP (Cat. #IT-03) to eliminate the noradrenergic nuclei. Using these data along with data acquired by elimination of serotonergic nuclei with 5,7-DHT, the authors showed that both noradrenergic nuclei in the locus coeruleus and serotonergic nuclei in the dorsal raphe nucleus express the alpha-7 nAChR subunit.

Related Products: Anti-DBH-SAP (Cat. #IT-03)