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Lesioning of medullary noradrenergic and adrenergic neurons using the immunotoxin anti-DBH-saporin.
Wrenn CC, Picklo MJ, Lappi DA, Robertson D, Wiley RG (1996) Lesioning of medullary noradrenergic and adrenergic neurons using the immunotoxin anti-DBH-saporin. Neuroscience 1996 Abstracts 749.15. Society for Neuroscience, Washington, DC.
Summary: Anti-DBH-saporin (α-DBH-sap) is an anti-neuronal immunotoxin comprised of an antibody against the noradrenaline synthesizing enzyme dopamine β-hydroxylase (DBH) coupled by a disulfide bond to the ribosome inactivating toxin saporin. This immunotoxin was injected into the left lateral ventricle of rats at doses of 5, 10, and 20 μg. After a two week survival time, the rats were sacrificed and the ability of the immunotoxin to lesion noradrenergic and adrenergic neurons was assessed by immunohistochemical staining for tyrosine hydroxylase (TH), DBH, and phenylethanolamine n-methyl transferase (PNMT). The locus coeruleus was completely lesioned at all three doses. The A5 and A7 cell groups were completely lesioned at the two higher doses. In the medulla, lesioning of the ventrolateral Al/Cl cell group and the dorsomedial A2/C2/C3 cell group was qualitatively observed to be incomplete at all three doses. Cell counts of TH+, DBH+, and PNMT+ neurons revealed that the number of neurons staining for each enzyme was reduced by the immunotoxin dose dependently. The ventrolateral population was lesioned more closely to completeness than the dorsomedial population. These data show that α-DBH-sap can be used to produce lesions of brainstem noradrenergic and adrenergic neurons. Supported by the Department of Veterans Affairs.
Related Products: Anti-DBH-SAP (Cat. #IT-03)
192 IgG-saporin lesion of basal forebrain cholinergic neurons in neonatal rats.
Pappas BA, Davidson CM, Fortin T, Nallathamby S, Park GA, Mohr E, Wiley RG (1996) 192 IgG-saporin lesion of basal forebrain cholinergic neurons in neonatal rats. Brain Res 96:52-61. doi: 10.1016/0165-3806(96)00095-8
Related Products: 192-IgG-SAP (Cat. #IT-01)
Trans-synaptic stimulation of cortical acetylcholine release after partial 192 IgG-saporin-induced loss of cortical cholinergic afferents.
Fadel J, Moore H, Sarter M, Bruno JP (1996) Trans-synaptic stimulation of cortical acetylcholine release after partial 192 IgG-saporin-induced loss of cortical cholinergic afferents. J Neurosci 16:6592-6600. doi: 10.1523/JNEUROSCI.16-20-06592.1996
Related Products: 192-IgG-SAP (Cat. #IT-01)
Intraseptal injection of the cholinergic immunotoxin 192-IgG saporin fails to disrupt latent inhibition in a conditioned taste aversion paradigm.
Dougherty KD, Salat D, Walsh TJ (1996) Intraseptal injection of the cholinergic immunotoxin 192-IgG saporin fails to disrupt latent inhibition in a conditioned taste aversion paradigm. Brain Res 736:260-269. doi: 10.1016/0006-8993(96)00712-3
Related Products: 192-IgG-SAP (Cat. #IT-01)
Differential effects of selective immunotoxic lesions of medial septal cholinergic cells on spatial working and reference memory.
Shen J, Barnes CA, Wenk GL, McNaughton BL (1996) Differential effects of selective immunotoxic lesions of medial septal cholinergic cells on spatial working and reference memory. Behav Neurosci 110:1181-1186. doi: 10.1037//0735-7044.110.5.1181
Related Products: 192-IgG-SAP (Cat. #IT-01)
Specific domains of ß-amyloid from Alzheimer plaque elicit neuron killing in human microglia.
Giulian D, Haverkamp LJ, Yu JH, Karshin W, Tom D, Li J, Kirkpatrick J, Kuo YM, Roher AE (1996) Specific domains of ß-amyloid from Alzheimer plaque elicit neuron killing in human microglia. J Neurosci 16:6021-6037. doi: 10.1523/JNEUROSCI.16-19-06021.1996 PMID: 8815885
Related Products: Acetylated LDL-SAP (Cat. #IT-08)
Extensive reinnervation of the hippocampus by embryonic basal forebrain neurons grafted into the septum of neonatal rats with selective cholinergic lesions.
Leanza G, Nikkah G, Nilsson OG, Wiley RG, Bjorklund A (1996) Extensive reinnervation of the hippocampus by embryonic basal forebrain neurons grafted into the septum of neonatal rats with selective cholinergic lesions. J Comp Neurol 373:355-357. doi: 10.1002/(SICI)1096-9861(19960923)373:3<355::AID-CNE4>3.0.CO;2-1
Related Products: 192-IgG-SAP (Cat. #IT-01)
Effects of neonatal lesions of the basal forebrain cholinergic system by 192 IgG-saporin: biochemical, behavioural and morphological characterization.
Leanza G, Nilsson OG, Nikkah G, Wiley RG, Bjorklund A (1996) Effects of neonatal lesions of the basal forebrain cholinergic system by 192 IgG-saporin: biochemical, behavioural and morphological characterization. Neuroscience 74:119-141. doi: 10.1016/0306-4522(96)00095-4
Related Products: 192-IgG-SAP (Cat. #IT-01)
The mitotoxin, basic fibroblast growth factor-saporin, effectively targets human prostatic carcinoma in an animal model
Davol P, Frackelton AR Jr (1996) The mitotoxin, basic fibroblast growth factor-saporin, effectively targets human prostatic carcinoma in an animal model. J Urol 156(3):1174-1179. doi: 10.1016/s0022-5347(01)65745-8
Related Products: FGF-SAP (Cat. #IT-38)
Injection of IgG 192-saporin into the medial septum produces cholinergic hypofunction and dose-dependent working memory deficits.
Walsh TJ, Herzog CD, Gandhi C, Stackman RW, Wiley RG (1996) Injection of IgG 192-saporin into the medial septum produces cholinergic hypofunction and dose-dependent working memory deficits. Brain Res 726:69-79. doi: 10.1016/0006-8993(96)00271-5
Related Products: 192-IgG-SAP (Cat. #IT-01)