Wrenn CC, Picklo MJ, Lappi DA, Robertson D, Wiley RG (1996) Lesioning of medullary noradrenergic and adrenergic neurons using the immunotoxin anti-DBH-saporin. Neuroscience 1996 Abstracts 749.15. Society for Neuroscience, Washington, DC.
Summary: Anti-DBH-saporin (α-DBH-sap) is an anti-neuronal immunotoxin comprised of an antibody against the noradrenaline synthesizing enzyme dopamine β-hydroxylase (DBH) coupled by a disulfide bond to the ribosome inactivating toxin saporin. This immunotoxin was injected into the left lateral ventricle of rats at doses of 5, 10, and 20 μg. After a two week survival time, the rats were sacrificed and the ability of the immunotoxin to lesion noradrenergic and adrenergic neurons was assessed by immunohistochemical staining for tyrosine hydroxylase (TH), DBH, and phenylethanolamine n-methyl transferase (PNMT). The locus coeruleus was completely lesioned at all three doses. The A5 and A7 cell groups were completely lesioned at the two higher doses. In the medulla, lesioning of the ventrolateral Al/Cl cell group and the dorsomedial A2/C2/C3 cell group was qualitatively observed to be incomplete at all three doses. Cell counts of TH+, DBH+, and PNMT+ neurons revealed that the number of neurons staining for each enzyme was reduced by the immunotoxin dose dependently. The ventrolateral population was lesioned more closely to completeness than the dorsomedial population. These data show that α-DBH-sap can be used to produce lesions of brainstem noradrenergic and adrenergic neurons. Supported by the Department of Veterans Affairs.
Related Products: Anti-DBH-SAP (Cat. #IT-03)