References

Gupta A, Anjomani-Virmouni S, Koundouros N, Dimitriadi M, Choo-Wing R, Valle A, Zheng Y, Chiu YH, Agnihotri S, Zadeh G, Asara JM, Anastasiou D, Arends MJ, Cantley LC, Poulogiannis G (2017) PARK2 depletion connects energy and oxidative stress to PI3K/Akt activation via PTEN S-Nitrosylation. Mol Cell 65(6):999-101.e7. doi: 10.1016/j.molcel.2017.02.019 PMID: 28306514

Objective: To investigate the missing piece in the dynamic signaling and metabolic network governing PI3K/Akt activation.

Summary: PARK2 inactivation connects energy and oxidative stress to Akt activation via redox-mediated inactivation of PTEN by S-nitrosylation to support cell survival under conditions of energy deprivation.

Usage: Immunoblotting

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Gholamin S, Mitra SS, Feroze AH, Liu J, Kahn SA, Zhang M, Esparza R, Richard C, Ramaswamy V, Remke M, Volkmer AK, Willingham S, Ponnuswami A, McCarty A, Lovelace P, Storm TA, Schubert S, Hutter G, Narayanan C, Chu P, Raabe EH, Harsh G 4th, Taylor MD, Monje M, Cho YJ, Majeti R, Volkmer JP, Fisher PG, Grant G, Steinberg GK, Vogel H, Edwards M, Weissman IL, Cheshier SH (2017) Disrupting the CD47-SIRPα anti-phagocytic axis by a humanized anti-CD47 antibody is an efficacious treatment for malignant pediatric brain tumors. Sci Transl Med 9(381):eaaf2968. doi: 10.1126/scitranslmed.aaf2968 PMID: 28298418

Summary: A humanized anti-CD47 antibody has proven to be an effective treatment for malignant pediatric brain tumors by disrupting the CD47-SIRPα anti-phagocytic axis. This breakthrough therapy shows promise in improving outcomes for young patients with these challenging tumors.

Yu Y-Q, Barry DM, Hao Y, Liu X-T, Chen Z-F (2017) Molecular and neural basis of contagious itch behavior in mice. Science 355:1072. doi: 10.1126/science.aak9748

Summary: The authors selectively ablated the SCN gastrin-releasing peptide receptor (GRPR) neurons using Bombesin-SAP (Cat. #IT-40), a peptide-conjugated toxin that kills GRPR neurons in the spinal cord. After bilateral injection of Bombesin-SAP into the SCN, immunohistochemistry showed that Bombesin-SAP injection resulted in ablation of SCN GRPR+ neurons.

Related Products: Bombesin-SAP (Cat. #IT-40)

Maurice T, Goguadze N (2017) Sigma-1 (σ1) receptor in memory and neurodegenerative diseases. Handb Exp Pharmacol 244:81-108. doi: 10.1007/164_2017_15

Related Products: 192-IgG-SAP (Cat. #IT-01)

Somasundaram P, Wyrick G, Fernandez D, Ghahari A, Pinhal C, Simmonds Richardson M, Rupp A, Cui L, Wu Z, Brown R, Badea T, Hattar S, Robinson P (2017) C-terminal phosphorylation regulates the kinetics of a subset of melanopsin-mediated behaviors in mice. Proc Natl Acad Sci U S A 114:2741-2746. doi: 10.1073/pnas.1611893114 PMID: 28223508

Summary: The authors show that the melanopsin photoresponse shutoff due to C-terminal phosphorylation determines the kinetics of the intrinsic light response in ipRGCs, the PLR, and reentrainment, but not masking and phase angle of entrainment. Immunofluorescence was performed using rabbit Anti-Melanopsin (1:1,000, Cat. #AB-N38) as the primary antibody with a 2-d incubation period, followed by goat anti-rabbit IgG 488 as the secondary antibody.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Jeong D, Lee J, Chang W, Chang J (2017) Identifying the appropriate time for deep brain stimulation to achieve spatial memory improvement on the Morris water maze. BMC Neuroscience 18:29.. doi: 10.1186/s12868-017-0345-4

Summary: This study was performed to determine the stage of memory affected by medial septum deep brain stimulation (MS-DBS). Memory impairment due to cholinergic denervation can be improved by DBS. The improvement is significantly correlated with the up-regulation of BDNF expression and neurogenesis. Based on the results of this study, the use of MS-DBS during the early stage of disease may restore spatial memory impairment.

Usage: Rats were injected bilaterally with 8 μl of 192-IgG-SAP (0.63 μg/μl) at the cerebroventricle.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Burma NE, Bonin RP, Leduc-Pessah H, Baimel C, Cairncross ZF, Mousseau M, Shankara JV, Stemkowski PL, Baimoukhametova D, Bains JS, Antle MC, Zamponi GW, Cahill CM, Borgland SL, De Koninck Y, Trang T (2017) Blocking microglial pannexin-1 channels alleviates morphine withdrawal in rodents. Nat Med 23:355-360.. doi: 10.1038/nm.4281

Summary: The authors investigated the mechanisms underlying opiate withdrawal in rat. Depletion of spinal lumbar microglia by intrathecal injections of Mac-1–SAP (Cat. #IT-33; 20 mcg) decreased withdrawal behaviors and attenuated the severity of withdrawal without affecting morphine antinociception. Unconjugated Saporin (Cat. #PR-01; 20 mcg) was used as control and had no effect on spinal CD11b immunoreactivity or naloxone-induced morphine withdrawal.

Related Products: Mac-1-SAP rat (Cat. #IT-33), Saporin (Cat. #PR-01)

Bondarenko N, Dilmukhametova L, Kurina A, Murtazina A, Sapronova A, Sysoeva A, Ugrumov M (2017) Plasticity of central and peripheral sources of noradrenaline in rats during ontogenesis. Biochemistry (Mosc) 82:373-379.. doi: 10.1134/S0006297917030166

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Hayashi R, Ishikawa Y, Katori R, Sasamoto Y, Taniwaki Y, Takayanagi H, Tsujikawa M, Sekiguchi K, Quantock AJ, Nishida K (2017) Coordinated generation of multiple ocular-like cell lineages and fabrication of functional corneal epithelial cell sheets from human iPS cells. Nat Protoc 12:683-696. doi: 10.1038/nprot.2017.007 PMID: 28253236

Usage: IHC 1:100

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Martínez-Gardeazabal J, González de San Román E, Moreno-Rodríguez M, Llorente-Ovejero A, Manuel I, Rodríguez-Puertas R (2017) Lipid mapping of the rat brain for models of disease. Biochim Biophys Acta Biomembr 1859:1548-1557.. doi: 10.1016/j.bbamem.2017.02.011

Objective: To map the spatial distribution of different lipid species in the rat central nervous system (CNS) using IMS to find a possible relationship between anatomical localization and physiology. The data obtained were subsequently applied to a model of neurological disease, the 192IgG-saporin lesion model of memory impairment.

Summary: The specific distribution of different lipids supports their involvement not only in structural and metabolic functions but also as intracellular effectors or specific receptor ligands and/or precursors. Moreover, the specific localization in the CNS described here will enable us to analyze lipid distribution to identify their physiological conditions in rat models of neurodegenerative pathologies, such as Alzheimer’s disease.

Usage: 192 IgG-SAP in aCSF (135 ng/1 μl/hemisphere; 0.25 μl/min) was administered.

Related Products: 192-IgG-SAP (Cat. #IT-01)