References

Sadhukhan R, Brown N, Ouellette D, Banach D, Filoti DI, Winarta D, Raghavendra R, Sousa S, Darcy A, Alessandri L, Ivanov A, Bose S, Eaton L, Preston G, Freeman J, Correia I (2018) Engineering elastic properties into an anti-TNFα monoclonal antibody. Cogent Biol 4(1):1469387. doi: 10.1080/23312025.2018.1469387

Objective: To engineer elastic properties into a TNFalpha antibody.

Summary: The results presented in this report with an anti-TNFα ELP mAb are a foundation for building on a new generation of fusion ELP mAbs, or other formats, that are stable, active, responsive to cues in local environment, and, with the FcRn mutation, cleared rapidly from circulation. More detailed studies are warranted to identify the appropriate ELP sequences for IA delivery, calculate residence time in the IA space, and demonstrate pharmacodynamics effect of the ELP-fusion protein.

Usage: Fab-ZAP human was mixed with anti-TNFα-ELP fusion monoclonal.

Related Products: Fab-ZAP human (Cat. #IT-51)

Sánchez-Migallón MC, Valiente-Soriano FJ, Nadal-Nicolás FM, Di Pierdomenico J, Vidal-Sanz M, Agudo-Barriuso M (2018) Survival of melanopsin expressing retinal ganglion cells long term after optic nerve trauma in mice. Exp Eye Res 174:93-97. doi: 10.1016/j.exer.2018.05.029 PMID: 29856984

Summary: Melanopsin-positive retinal ganglion cells (RGCs) do not respond to axotomy in the same way than the rest of RGCs, and so while image-forming RGCs die in two exponential phases, non-image forming RGCs die only during the first quick phase.

Usage: Retinas were dissected as flat mounts and double-immunostained against melanopsin (1:5000).

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Motohashi T, Kunisada T (2019) Direct conversion of mouse embryonic fibroblasts into neural crest cells. (eds. Turksen K). In: Skin Stem Cells. Methods in Molecular Biology. 1879:307-321. Humana Press, New York, NY. doi: 10.1007/7651_2018_145 PMID: 29797008

Objective: To describe methods for the direct conversion of mouse embryonic fibroblasts into neural crest cells (NCCs).

Summary: Sox10-IRES-Venus mouse fibroblasts were used for the conversion and isolation of converted NCCs as SOX10-positive cells.

Usage: Immunostaining for Flow Cytometry

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Staib JM, Della Valle R, Knox DK (2018) Disruption of medial septum and diagonal bands of Broca cholinergic projections to the ventral hippocampus disrupt auditory fear memory. Learn Mem 152:71-79. doi: 10.1016/j.nlm.2018.05.009

Objective: To determine which efferent projections are critical for contextual fear memory discrimination and extinction memory.

Summary: The results of this study suggest that MS/vDBB cholinergic neurons are critical for fear and extinction memory.

Usage: 192-IgG saporin was infused into all brain regions at a concentration of 0.2 μg/μL dissolved in 0.2 M PBS. The total volume of each injection was 0.5 μL. Sham surgeries were accomplished using the same volume (0.5 μL) of PBS.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Dhez AC, Benedetti E, Antonosante A, Panella G, Ranieri B, Florio TM, Cristiano L, Angelucci F, Giansanti F, Di Leandro L, d’Angelo M, Melone M, De Cola A, Federici L, Galzio R, Cascone I, Raineri F, Cimini A, Courty J, Giordano A, Ippoliti R (2018) Targeted therapy of human glioblastoma via delivery of a toxin through a peptide directed to cell surface nucleolin. J Cell Physiol 233(5):4091-4105. doi: 10.1002/jcp.26205 PMID: 28941284

Wüstemann T, Haberkorn U, Babich J, Mier W (2019) Targeting prostate cancer: Prostate-specific membrane antigen based diagnosis and therapy. Med Res Rev 39(1):40-69. doi: 10.1002/med.21508 PMID: 29771460

Summary: Conjugation to the antibody was achieved by reacting the biotinylated humanized antibody to prostate-specific membrane antigen (PMSA) with Streptavidin-ZAP. Binding potency of the conjugate was comparable to that of the naked antibody and in vivo experiments proved potent for selective tumor growth inhibition in mice bearing LNCaP tumors.

Related Products: Streptavidin-ZAP (Cat. #IT-27)

See Also:

• Kuroda K et al. Saporin toxin-conjugated monoclonal antibody targeting prostate-specific membrane antigen has potent anticancer activity. Prostate 70(12):1286-1294, 2010.

Eng MS, Kaur J, Prasmickaite L, Engesaeter BO, Weyergang A, Skarpen E, Berg K, Rosenblum MG, Maelandsmo GM, Hogset A, Ferrone S, Selbo PK (2018) Enhanced targeting of triple-negative breast carcinoma and malignant melanoma by photochemical internalization of CSPG4-targeting immunotoxins. Photochem Photobiol Sci 17:539-551. doi: 10.1039/C7PP00358G

Summary: The combination of the drug delivery technology PCI and CSPG4-targeting immunotoxins is an efficient, specific and light-controlled strategy for the elimination of aggressive cells of TNBC and malignant melanoma origin. This study lays the foundation for further preclinical evaluation of PCI in combination with CSPG4-targeting.

Usage: To obtain the immunotoxin 225.28-saporin, Streptavidin-Saporin (Cat. #IT-27; Streptavidin-ZAP), with an average of 2.5 molecules of saporin per molecule of streptavidin, was combined with biotinylated 225.28, a CSPG4-specific mouse mAb, IgG2a.

Related Products: Streptavidin-ZAP (Cat. #IT-27)

Sun X, Zhu Y, Yin HY, Guo ZY, Xu F, Xiao B, Jiang WL, Guo WM, Meng HY, Lu SB, Wang Y, Peng J (2018) Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function. Stem Cell Res Ther 9:133. doi: 10.1186/s13287-018-0884-3 PMID: 29751848

Objective: To improve the traditional methods for inducing the differentiation of mesenchymal stromal cells (MSCs) into Schwann cell-like cells (SCLCs).

Summary: Results indicated that the intermittent induction method is more efficient than traditional methods for inducing adipose-derived stem cells to differentiate into SCLCs.

Usage: immunofluorescence (1:500)

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Araldi D, Ferrari LF, Levine JD (2018) Role of GPCR (mu-opioid)-receptor tyrosine kinase (epidermal growth factor) crosstalk in opioid-induced hyperalgesic priming (type II). Pain 159(5):864-875. doi: 10.1097/j.pain.0000000000001155

Objective: To determine the the mechanisms mediating the induction of opioid-induced hyperalgesia and the prolongation of prostaglandinE2-induced hyperalgesia in type II hyperalgesic priming.

Summary: Understanding the mechanisms responsible for the induction of type II hyperalgesic priming, a form of neuroplasticity in the peripheral terminal of the primary afferent nociceptor, may provide useful information for the design of drugs with improved therapeutic profiles to treat neuroplasticity induced by chronic use of opioids.

Usage: SSP-SAP was prepared in saline (5 ng/mL), and 20 mL was injected intrathecally into rats, 14 days before nociceptive tests.

Related Products: SSP-SAP (Cat. #IT-11)

Cho JS, Lee J, Jeong DU, Kim HW, Chang WS, Moon J, Chang JW (2018) Effect of placenta-derived mesenchymal stem cells in a dementia rat model via microglial mediation: A comparison between stem cell transplant methods. Yonsei Med J 59:406-415. doi: 10.3349/ymj.2018.59.3.406

Objective: To study the therapeutic effects of human placenta-derived mesenchymal stem cells (pMSCs) in a dementia rat model using either intracerebroventricular (ICV) or intravenous (IV) injections and analyze their mechanisms of therapeutic action.

Summary: ICV and IV injections of pMSCs facilitate the recovery of cholinergic neuronal populations and cognitive behavior. This recovery likely occurs through paracrine effects that resemble microglia function rather than direct differentiation of injected pMSCs into cholinergic neurons.

Usage: Dementia modeling was established by bilateral ventricle infusion of 192 IgG-SAP to lesion cholinergic neurons. 8 μL of 192 IgG-saporin (0.63 μg/μL) were bilaterally injected into the lateral ventricle at a rate of 1 μL/min and was left to diffuse for 5 min after injection. Rats were subjected to the Morris water maze and subsequent immunostaining analyses.

Related Products: 192-IgG-SAP (Cat. #IT-01)