References

Acton D, Ren X, DiCostanzo S, Dalet A, Bourane S, Bertocchi I, Eva C, Goulding M (2019) Spinal neuropeptide Y1 receptor-expressing neurons form an essential excitatory pathway for mechanical itch. Cell Reports 28(3):625-639.e6 . doi: 10.1016/j.celrep.2019.06.033

Objective: To determine the central pathway for mechanical itch.

Summary: NPY-Y1 signaling regulates the transmission of innocuous tactile information by establishing biologically relevant thresholds for touch discrimination and mechanical itch reflexes. Neither the evoked nor spontaneous scratching seen following activation of Y1Cre neurons was affected by ablation of the GRPR+ neurons. NK1R+ neuron ablation failed to modulate mechanically evoked itch.

Usage: P28 mice were given a single intrathecal (i.t.) injection of either Bombesin-SAP (400 ng in 5 mL 0.9% sterile saline) to ablate GRPR+ cells or SSP-SAP to ablate NK1r+ neurons (100 ng in 5 mL 0.9% sterile saline). Littermate controls received Blank-SAP (equal mass in 5 mL 0.9% sterile saline).

Related Products: Bombesin-SAP (Cat. #IT-40), SSP-SAP (Cat. #IT-11), Blank-SAP (Cat. #IT-21)

Wu SJ, Coarfa C, Abbas H, Checker R, Dhar S, Rajapakshe K, Lee MG, Flores ER (2019) ∆Np63 executes an oncogenic program through the regulation of a common landscape of super-enhancer associated genes in non-small cell lung cancer. Cell Reports 3420369. doi: 10.2139/ssrn.3420369

Objective: To investigate how ΔNp63 serves to maintain lung adenocarcinoma (ADC) and squamous cell carcinoma (SCC) through the regulation and maintenance of lung stem cells that are necessary for lung adenocarcinoma and squamous cell initiation and progression.

Summary: ∆Np63 maintains lung ADC and SCC by keeping lung stem cells in quiescence. ChIP-seq analysis of lung basal cells and alveolar type 2 (AT2) cells lacking ∆Np63 revealed a robust loss of activating histone marks at super enhancers of cell identity genes defining a unifying oncogenic role for ∆Np63 in non-small cell lung cancer.

Usage: staining

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Chaskiel L, Bristow AD, Bluthé RM, Dantzer R, Blomqvist A, Konsman JP (2019) Interleukin-1 reduces food intake and body weight in rat by acting in the arcuate hypothalamus. Brain Behav Immun 81:560-573. doi: 10.1016/j.bbi.2019.07.017

Summary: The authors tested the hypothesis that IL-1R1 expressing cells in the ARH mediate IL-1β and/or LPS-induced hypophagia in the rat.

Related Products: Custom Conjugates, Saporin (Cat. #PR-01)

Verweij J, Chaney SY, Bredl D, Vemaraju S, König GM, Kostenis E, Lang RA, Copenhagen DR (2019) Light responses of melanopsin-expressing ganglion cells in the foetal mammalian retina. bioRxiv 675702. doi: 10.1101/675702

Related Products: Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)

Ikeda K, Onimaru H, Inada H, Tien Lin S, Arata S, Osumi N (2019) Structural and functional defects of the respiratory neural system in the medulla and spinal cord of Pax6 mutant rats. Brain Res Bull 152:107-116. doi: 10.1016/j.brainresbull.2019.07.007 PMID: 31301380

Objective: To determine whether the respiratory center in the medulla functions normally in Pax6 mutant rats.

Summary: The Pax6 gene is involved in the functional development of the medullary respiratory center.

Usage: Anti-NK1r was used in immunofluorescence (1:800).

Related Products: NK-1 Receptor Rabbit Polyclonal, affinity-purified (Cat. #AB-N33AP)

Chew C, Sengelaub DR (2019) Neuroprotective effects of exercise on the morphology of somatic motoneurons following the death of neighboring motoneurons. Neurorehabil Neural Repair 33(8):656-667. doi: 10.1177/1545968319860485

Objective: To explore whether exercise shows the same neuroprotective effect on induced dendritic atrophy as that seen with androgen treatment.

Summary: Exercise following neural injury exerts a protective effect on motoneuron dendrites comparable to that seen with exogenous androgen treatment.

Usage: Motoneurons innervating the left vastus medialis muscle were selectively killed by intramuscular injection of CTB-SAP (2 μL, 0.1%). Saporin injection reduced the weight of the vastus medialis muscle; exercise had no effect on muscle weight.

Related Products: CTB-SAP (Cat. #IT-14)

Chen P, Huang Y, Wang Y, Li S, Chu H, Rong M (2019) MALAT1 overexpression promotes the proliferation of human periodontal ligament stem cells by upregulating fibroblast growth factor 2. Exp Ther Med 18(3):1627-1632. doi: 10.3892/etm.2019.7748 PMID: 31410118

Usage: western (1:1300)

Related Products: Fibroblast Growth Factor Rabbit Polyclonal, mammalian (Cat. #AB-07)

Wang Z, Wu J-L, Zhong F, Liu Y, Yu Y-Q, Sun J-J, Wang S, Li H, Zhou X-F, Hu Z-L, Dai R-P (2019) Upregulation of proBDNF in the mesenteric lymph nodes in septic mice. Neurotox Res 36(3):540-550. doi: 10.1007/s12640-019-00081-3 PMID: 31278527

Objective: To characterize the expression pattern of proBDNF in mesenteric lymph nodes (MLNs) after lipopolysaccharide (LPS) stimulation, and to investigate the association of pathogenesis of sepsis.

Summary: This study provided direct evidence that sepsis promoted the proliferation of CD3+, CD4+, and CD8+ T cells, but inhibits CD19+ B cells in the MLNs. Also, proBDNF was upregulated during the sepsis, which was mainly localized on CD3+ and CD4+ T cells in MLNs. The upregulation of proBDNF signaling and cytokines activation in the MLNs may be involved in the disease progress of sepsis.

Usage: western blot (1:2000)

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Nizari S, Carare RO, Romero IA, Hawkes CA (2019) 3D reconstruction of the neurovascular unit reveals differential loss of cholinergic innervation in the cortex and hippocampus of the adult mouse brain. Front Aging Neurosci 11:172. doi: 10.3389/fnagi.2019.00172

Objective: To further characterize the effect of the loss of cholinergic innervation on the NVU (neurovascular unit) in Alzheimer’s Disease.

Summary: Significantly less ChAT staining was detected in the medial septum of saporin-treated mice at 45 days post-surgery. This was accompanied by a significant decrease in cholinergic nerve fiber density in the hippocampus and the cortex. As expected, p75 NTR-negative neurons in the striatum were not affected by mu p75-SAP treatment.

Usage: In this study, the mu-p75-SAP was used to induce death of basal forebrain cholinergic neurons and their fiber projections. mu p75-SAP 0.5 µL (0.596 µg/µL) or 0.9% saline (n = 19) was injected into each ventricle.

Related Products: mu p75-SAP (Cat. #IT-16)

Hernández-Melesio MA, Alcaraz-Zubeldia M, Jiménez-Capdeville ME, Martínez-Lazcano JC, Santoyo-Pérez ME, Quevedo-Corona L, Gerónimo-Olvera C, Sánchez-Mendoza A, Ríos C, Pérez-Severiano F (2019) Nitric oxide donor molsidomine promotes retrieval of object recognition memory in a model of cognitive deficit induced by 192 IgG-saporin. Behav Brain Res 366:108-117. doi: 10.1016/j.bbr.2019.03.031

Objective: To analyze the potential of a NO donor (molsidomine, MOLS) to prevent the recognition memory deficits resulting from the septal cholinergic denervation by 192-IgG-SAP in rats.

Summary: Results showed that 192-IgG-SAP reduced the immunoreactivity of cholinergic septal neurons (41%), compared with PBS-receiving control rats (p < 0.05).

Usage: The injection reached the medial septum (MS) structure with 192-IgG-SAP diluted in PBS solution (0.22 μg in 1μl) or PBS as control, both at 0.25 μl/min, allowing diffusion for 3 min, AP+0.6, L 0.0, V−7.0 from Bregma.

Related Products: 192-IgG-SAP (Cat. #IT-01)