References

Ma YP, Yang Y, Jiang SM, Liu L, Zhang Z, Wang YN, Zou GM, Li WG (2020) Angiotensin II type 1 receptor blockers favorably affect renal angiotensin II and MAS receptor expression in patients with diabetic nephropathy. J Renin Angiotensin Aldosterone Syst 21(2):1470320320919607. doi: 10.1177/1470320320919607 PMID: 32370637

Objective: To assess the renal expression of angiotensin II type 1 receptor (AT1R), angiotensin II type 2 receptor (AT2R), and MAS receptor in human type 2 diabetic nephropathy (DN).

Summary: Renal expression levels of AT1R increase during the early stages of DN, ARB reducing AT1R while increasing MAS receptor. Therefore, ARB should be used as soon as possible in patients with DN.

Usage: Immunohistochemistry

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Zhou G, Li J, Zeng T, Yang P, Li A (2020) The regulation effect of WNT-RAS signaling in hypothalamic paraventricular nucleus on renal fibrosis. J Nephrol 33(2):289-297. doi: 10.1007/s40620-019-00637-8 PMID: 31392659

Objective: To investigate whether abnormal expression of central wnt/β-catenin signaling and renin-angiotensin system (WNT-RAS signaling) was tightly involved in chronic kidney disease (CKD).

Summary: These data suggest that the central WNT-RAS signaling plays a significant role in the development and progression of CKD.

Usage: immunohistochemistry and western blot

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Thal LB, Kovtun O, Rosenthal SJ (2020) Labeling neuronal proteins with quantum dots for single-molecule imaging. (eds. Fontes A, Santos B). In: Quantum Dots. Methods in Molecular Biology. 2135:169-177. Humana Press, New York, NY. doi: 10.1007/978-1-0716-0463-2_9 PMID: 32246334

Objective: The authors describe how nanometer-sized fluorescent semiconductors called quantum dots (QD) can be used to label neuronal proteins in a single QD imaging format.

Usage: Secondary antibody-conjugated QDs target membrane proteins pre-treated with SERT Mouse Monoclonal.

Related Products: SERT Mouse Monoclonal (Cat. #AB-N40)

Volobueva MN, Dobryakova YV, Manolova AO, Stepanichev MY, Kvichansky AA, Gulyaeva NV, Markevich VA, Bolshakov AP (2020) Intracerebroventricular administration of 192IgG-saporin alters the state of microglia in the neocortex. Neurochem J 14(1):37-42. doi: 10.1134/S1819712420010213

Objective: The effect of intracerebroventricular (icv) immunotoxin administration on the state of microglia in tissues adjacent to the ventricle (striatum and parietal cortex) and remotely located but receiving innervation from the medial septal region and diagonal band of Broca (entorhinal cortex and olfactory bulbs).

Summary: 1.5 months after the administration of immunotoxin, microglia are activated only in the neocortical areas, not in the striatum or olfactory bulbs.

Usage: Injected bilaterally at a dose of 4 μg in each ventricle.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Wang F, Jing P, Zhan P, Zhang H (2020) Thyroid hormone in the pathogenesis of congenital intestinal dysganglionosis. Pediatr Dev Pathol 23(4):285-295. doi: 10.1177/1093526620908984 PMID: 32212960

Objective: To investigate the role of thyroid hormone (TH) in the pathogenesis of intestinal dysganglionosis (ID).

Summary: 6-propyl-2-thiouracil (PTU) inhibited TH production, reduced the number of enteric neurons, impaired intestinal motility, and impeded ENCC mitosis in zebrafish, suggesting a possible role of CH in the pathogenesis of ID.

Usage: Immunostaining (1:60); AB-N01AP: NGFr (mu p75) Rabbit Polyclonal

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Ruggiero FM, Rodríguez-Walker M, Daniotti JL (2020) Exploiting the internalization feature of an antibody against the glycosphingolipid SSEA-4 to deliver immunotoxins in breast cancer cells. Immunol Cell Biol 98(3):187-202. doi: 10.1111/imcb.12314 PMID: 31916611

Qian L, Kasas L, Milne MR, Rawashdeh O, Marks N, Sharma A, Bellingham MC, Coulson EJ (2020) Cholinergic basal forebrain degeneration due to obstructive sleep apnoea increases Alzheimer’s pathology in mice. bioRxiv 2020.03.12.989848. doi: 10.1101/2020.03.12.989848

Usage: bilateral injections of urotensin II-saporin (UII-SAP; 0.07 μg/μL per site – unless stated otherwise; generous gift from Advanced Targeting Systems)

Related Products: Custom Conjugates, Blank-SAP (Cat. #IT-21)

Qin J, Wang M, Zhao T, Xiao X, Li X, Yang J, Yi L, Goffinet AM, Qu Y, Zhou L (2020) Early forebrain neurons and scaffold fibers in human embryos. Cerebral Cortex 30(3):913–928. doi: 10.1093/cercor/bhz136 PMID: 31298263

Objective: To study neural progenitor proliferation, neuronal migration, areal organization, and pioneer axon wiring are critical events during early forebrain development.

Summary: Axons from pioneer neurons in prethalamus, ventral telencephalon, and cortical preplate cross the diencephalon–telencephalon junction and the pallial–subpallial boundary, forming scaffolds that could guide thalamic and cortical axons at later stages.

Usage: Immunofluorescence (1:600); AB-N01AP: NGFr (mu p75) Rabbit Polyclonal, affinity-purified

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Barry DM, Liu XT, Liu B, Liu XY, Gao F, Zeng X, Liu J, Yang Q, Wilhelm S, Yin J, Tao A, Chen ZF (2020) Exploration of sensory and spinal neurons expressing gastrin-releasing peptide in itch and pain related behaviors. Nat Commun 11(1):1397. doi: 10.1038/s41467-020-15230-y

Objective: To determine the role of GRP in sensory neurons.

Summary: GRP is a neuropeptide in sensory neurons for nonhistaminergic itch, and GRP sensory neurons are dedicated to itch transmission.

Usage: Bombesin-SAP (200 ng/5 μL, i.t.) was injected 2 weeks prior to optical stimulation.

Related Products: Bombesin-SAP (Cat. #IT-40)

Bernabe CS, Caliman IF, Truitt WA, Molosh AI, Lowry CA, Hay-Schmidt A, Shekhar A, Johnson PL (2020) Using loss- and gain-of-function approaches to target amygdala-projecting serotonergic neurons in the dorsal raphe nucleus that enhance anxiety-related and conditioned fear behaviors. J Psychopharmacol 34(4):400-411. doi: 10.1177/0269881119900981

Objective: To investigate the role of amygdala-projecting 5-HT neurons in the DR in innate anxiety and conditioned fear behaviors.

Summary: The studies support the hypothesis that amygdala-projecting 5-HT neurons in the DR represent an anxiety and fear-on network.

Usage: Each rat received two bilateral microinjections per site (100 nL each, 1 μM in artificial cerebrospinal fluid) of either Anti-SERT-SAP or the control Mouse IgG-SAP.

Related Products: Anti-SERT-SAP (Cat. #IT-23), Mouse IgG-SAP (Cat. #IT-18)