- Home
- Knowledge Base
- References
Extensive immunolesions of basal forebrain cholinergic system impair offspring recognition in sheep.
Ferreira G, Meurisse M, Gervais R, Ravel N, Levy F (2001) Extensive immunolesions of basal forebrain cholinergic system impair offspring recognition in sheep. Neuroscience 106(1):103-116. doi: 10.1016/s0306-4522(01)00265-2
Summary: Through the use of 192-Saporin (Cat. #IT-01) the association of basal forebrain cholinergic neurons to learning instrumental tasks has been well established in the rat. The authors wished to examine whether these neurons were also associated with social learning tasks, such as offspring recognition in sheep. Using ME20.4-SAP (Cat. #IT-15) the basal forebrain cholinergic neurons of sheep were lesioned by intraventricular bilateral injections (150 µg). The results demonstrate that these neurons contribute to visual discrimination learning, and are involved in formation of lamb recognition memory.
Related Products: 192-IgG-SAP (Cat. #IT-01), ME20.4-SAP (Cat. #IT-15)
Distribution and co-localization of choline acetyltransferase and p75 neurotrophin receptors in the sheep basal forebrain: implications for the use of a specific cholinergic immunotoxin.
Ferreira G, Meurisse M, Tillet Y, Lévy F (2001) Distribution and co-localization of choline acetyltransferase and p75 neurotrophin receptors in the sheep basal forebrain: implications for the use of a specific cholinergic immunotoxin. Neuroscience 104(2):419-439. doi: 10.1016/s0306-4522(01)00075-6 PMID: 11377845
Summary: ME20.4 is a monoclonal antibody (Cat. #AB-N07) that has been shown to bind the p75 receptor in rabbit, sheep, dog, cat, raccoon, pig, and several primate species. Ferreira et al. investigate ME20.4-SAP (bilateral, 150 µl per ventricle, 50-150 µg total; Cat. #IT-15) use in sheep to assess distribution and localization of p75. The authors demonstrate 80-95% loss of basal forebrain cholinergic neurons and acetylcholinesterase-positive fibers in the hippocampus, olfactory bulb, and entorhinal cortex.
Related Products: ME20.4-SAP (Cat. #IT-15), NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)
Crossed unilateral lesions of the cholinergic basal forebrain (by me20.4IgG-saporin) and fornix from inferior temporal cortex produce severe learning impairments in rhesus monkeys.
Easton A, Ridley RM, Baker HF, Gaffan D (2000) Crossed unilateral lesions of the cholinergic basal forebrain (by me20.4IgG-saporin) and fornix from inferior temporal cortex produce severe learning impairments in rhesus monkeys. Neuroscience 2000 Abstracts 205.8. Society for Neuroscience, New Orleans, LA.
Summary: Section of the anterior temporal stem, amygdala and fornix in monkeys results in a dense anterograde amnesia. We have proposed that this impairment is a result of isolating the medial temporal lobe and inferior temporal cortex from their basal forebrain afferents. Evidence suggests that the cholinergic cells of the basal forebrain are important for learning and memory. In the present experiment we have made an immunotoxic unilateral lesion (ME20.4IgG-saporin), specific for the cholinergic cells of the basal forebrain, in combination with unilateral fornix damage in the same hemisphere, and a lesion of the inferior temporal cortex in the opposite hemisphere to disconnect these cholinergic cells from the medial temporal lobe and inferior temporal cortex. These monkeys were severely impaired at scene learning and concurrent visual discrimination learning, both of which are sensitive to sections of the anterior temporal stem, amygdala and fornix. This deficit is strongly correlated with the degree of acetylcholine loss in the basal forebrain in the hemisphere with the immunotoxic lesion. This result strengthens the proposal that the cholinergic cells of the basal forebrain are essential for new learning, and that their interaction with the medial temporal lobe and inferior temporal cortex is required for normal learning in monkey and man.
Related Products: ME20.4-SAP (Cat. #IT-15)
The role of cortical cholinergic afferent projections in cognition: impact of new selective immunotoxins.
McGaughy J, Everitt BJ, Robbins TW, Sarter M (2000) The role of cortical cholinergic afferent projections in cognition: impact of new selective immunotoxins. Behav Brain Res 115:251-263. doi: 10.1016/s0166-4328(00)00262-x
Summary: Review and comparison of immunolesioning techniques.
Related Products: 192-IgG-SAP (Cat. #IT-01), ME20.4-SAP (Cat. #IT-15)
Featured Article: CBF lesioning in rabbits
Beach T (2000) Featured Article: CBF lesioning in rabbits. Targeting Trends 1(1)
Related Products: ME20.4-SAP (Cat. #IT-15)
Read the featured article in Targeting Trends.
See Also:
Synergistic effects of unilateral immunolesions of the cholinergic projections from the basal forebrain and contralateral ablations of the inferotemporal cortex and hippocampus in monkeys.
Barefoot HC, Baker HF, Ridley RM (2000) Synergistic effects of unilateral immunolesions of the cholinergic projections from the basal forebrain and contralateral ablations of the inferotemporal cortex and hippocampus in monkeys. Neuroscience 98(2):243-251. doi: 10.1016/s0306-4522(00)00131-7
Related Products: ME20.4-SAP (Cat. #IT-15)
Cortical cholinergic denervation elicits vascular A beta deposition.
Roher AE, Kuo YM, Potter PE, Emmerling MR, Durham RA, Walker DG, Sue LI, Honer WG, Beach TG (2000) Cortical cholinergic denervation elicits vascular A beta deposition. Ann NY Acad Sci 903:366-373. doi: 10.1111/j.1749-6632.2000.tb06388.x
Related Products: ME20.4-SAP (Cat. #IT-15)
Cholinergic deafferentation of the rabbit cortex: a new animal model of Abeta deposition.
Beach TG, Potter PE, Kuo YM, Emmerling MR, Durham RA, Webster SD, Walker DG, Sue LI, Scott S, Layne KJ, Roher AE (2000) Cholinergic deafferentation of the rabbit cortex: a new animal model of Abeta deposition. Neurosci Lett 283:9-12. doi: 10.1016/s0304-3940(00)00916-2
Related Products: ME20.4-SAP (Cat. #IT-15)
Severe learning impairment caused by combined immunotoxic lesion of the cholinergic projections to the cortex and hippocampus in monkeys.
Ridley RM, Pugh P, Maclean CJ, Baker HF (1999) Severe learning impairment caused by combined immunotoxic lesion of the cholinergic projections to the cortex and hippocampus in monkeys. Brain Res 836:120-138. doi: 10.1016/s0006-8993(99)01641-8
Related Products: ME20.4-SAP (Cat. #IT-15)
Different effects on learning ability after injection of the cholinergic immunotoxin ME20.4 IgG-saporin into the diagonal band of Broca, basal nucleus of Meynert, or both in monkeys.
Ridley RM, Barefoot HC, MacLean CJ, Pugh P, Baker HF (1999) Different effects on learning ability after injection of the cholinergic immunotoxin ME20.4 IgG-saporin into the diagonal band of Broca, basal nucleus of Meynert, or both in monkeys. Behav Neurosci 113:303-315. doi: 10.1037//0735-7044.113.2.303
Related Products: ME20.4-SAP (Cat. #IT-15)