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Exercise promotes recovery after motoneuron injury via hormonal mechanisms.
Chew C, Sengelaub DR (2020) Exercise promotes recovery after motoneuron injury via hormonal mechanisms. Neural Regen Res 15(8):1373-1376. doi: 10.4103/1673-5374.274323
Objective: To describe how exercise is neuroprotective for motoneurons, accelerating axon regeneration following axotomy and attenuating dendritic atrophy following the death of neighboring motoneurons.
Summary: Exercise offers a simple, low barrier-to-entry behavioral intervention which is neuroprotective and pro-regenerative following neural injury.
Usage: Motoneurons innervating the left vastus medialis muscle were selectively killed by intramuscular injection of CTB-SAP (2 μL, 0.1%).
Related Products: CTB-SAP (Cat. #IT-14)
Proline hydroxylation primes protein kinases for autophosphorylation and activation
Lee SB, Ko A, Oh YT, Shi P, D’Angelo F, Frangaj B, Koller A, Chen EI, Cardozo T, Iavarone A, Lasorella A (2020) Proline hydroxylation primes protein kinases for autophosphorylation and activation. Mol Cell 79(3):376-389.e8. doi: 10.1016/j.molcel.2020.06.021 PMID: 32640193
Objective: To investigate the formation of prolyl-hydroxylated intermediates as a novel mechanism of kinase maturation and a general mechanism of regulation of CMGC kinases in eukaryotes.
Summary: Identified a highly conserved proline in the kinase domain of CMGC kinases that is hydroxylated by the PHD1 prolyl-hydroxylase, and this event precedes tyrosine autophosphorylation.
Usage: Western blot
Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)
The impact of bone cancer on the peripheral encoding of mechanical pressure stimuli.
Kucharczyk MW, Chisholm KI, Denk F, Dickenson AH, Bannister K, McMahon SB (2020) The impact of bone cancer on the peripheral encoding of mechanical pressure stimuli. Pain 161(8):1894-1905. doi: 10.1097/j.pain.0000000000001880 PMID: 32701848
Usage: immunohistochemistry (1:400)
Related Products: trkA Rabbit Polyclonal (Cat. #AB-N03)
Superior mouse eosinophil depletion in vivo targeting transgenic Siglec-8 instead of endogenous Siglec-F: Mechanisms and pitfalls.
Knuplez E, Krier-Burris R, Cao Y, Marsche G, O’Sullivan J, Bochner BS (2020) Superior mouse eosinophil depletion in vivo targeting transgenic Siglec-8 instead of endogenous Siglec-F: Mechanisms and pitfalls. J Leukoc Biol 108:43-58. doi: 10.1002/jlb.3hi0120-381r
Objective: To determine if targeting Siglec-8 with mouse IgG1 antibodies, rather than targeting Siglec-F with rat IgG antibodies, in mice transgenic for Siglec-8, will prove to be a more effective strategy for eliminating mouse eosinophils in vivo.
Summary: This study is the first to describe a novel mouse strain of Siglec-8+F− eosinophils—a useful tool for studying human Siglec biology in preclinical models.
Usage: Siglec-8+F− mouse eosinophils were pretreated with 10 μg/mL saporin-conjugated isotype controls (mouse IgG1 or rat IgG2), anti-Siglec-8 (2C4) or anti-Siglec-F (9C7) antibodies for 24 h.
Related Products: Custom Conjugates
Vitamin C decreases VEGF expression levels via hypoxia‑inducible factor‑1α dependent and independent pathways in lens epithelial cells.
Zhao L, Wang J, Zhang Y, Wang L, Yu M, Wang F (2020) Vitamin C decreases VEGF expression levels via hypoxia‑inducible factor‑1α dependent and independent pathways in lens epithelial cells. Mol Med Rep 22(1):436-444. doi: 10.3892/mmr.2020.11103 PMID: 32377733
Objective: To investigate the mechanisms underlying the effects of vitamin C on the expression levels of VEGF.
Summary: Vitamin C inhibits the cell proliferation and the expression levels of VEGF in lens epithelial cells following a co-treatment with the HIF-1 inhibitor.
Usage: Western blot (1:500)
Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)
Activation of the renin-angiotensin system in high fructose-induced metabolic syndrome.
Kim M, Do GY, Kim I (2020) Activation of the renin-angiotensin system in high fructose-induced metabolic syndrome. Korean J Physiol Pharmacol 24(4):319-328. doi: 10.4196/kjpp.2020.24.4.319 PMID: 32587126
Objective: To investigate the hypothesis that high fructose intake induces activation of the renin-angiotensin systems (RAS), resulting in hypertension and metabolic syndrome.
Summary: High fructose intake increased serum renin, Ang II, triglyceride, and cholesterol levels. High fructose intake increased the expression of angiotensinogen in the liver; angiotensin-converting enzyme in the lungs; and renin, angiotensin II type 1a receptor (AT1aR), and angiotensin II type 1b receptor (AT1bR) in the kidneys. High fructose intake induces activation of RAS, resulting in hypertension and metabolic syndrome.
Usage: Immunohistochemistry
Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)
Effects of adjuvant-induced arthritis on the ventral prostate of rats treated with angiotensin AT1 receptor blocker
da Rocha ES, Gomes FC, de Oliveira MR, Bruneto RV, Borges RMR, de Carvalho EP, Angeli-Junior EV, Garcia-Filho GF, Doná JPL, de Oliveira Batanero RP, Carlos CP, de Melo-Neto JS (2020) Effects of adjuvant-induced arthritis on the ventral prostate of rats treated with angiotensin AT1 receptor blocker. Life Sci 252:117629. doi: 10.1016/j.lfs.2020.117629 PMID: 32247619
Objective: To analyze the prostatic compartments, extracellular matrix, microvascularization, transforming growth factor-beta (TGF-β) and angiotensin II receptors type 1 (AT1) levels, and histopathology of the ventral prostate in a rat model for rheumatoid arthritis, and to evaluate the effect of angiotensin II AT1 receptor blocker (ARB) in the disease.
Summary: AIA may activate specific mechanisms that modify the prostatic environment; the use of ARB attenuates some altered prostate parameters in a rat model for arthritis.
Usage: Immunohistochemistry (1:500)
Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)
Integration of peripheral and central systems in control of ingestive and reproductive behavior
Schneider J (2020) Integration of peripheral and central systems in control of ingestive and reproductive behavior. Oxford Research Encyclopedia of Neuroscience . Oxford University Press doi: 10.1093/acrefore/9780190264086.013.23
Summary: Highly glucose-sensitive cells in the ventrolateral medulla send catecholaminergic projections to the PVH. These projections can be selectively destroyed by Anti-DBH-SAP “DSAP” experiments show that catecholaminergic projections from glucose-sensitive cells in the ventrolateral medulla are necessary for all responses to glucoprivation, including increases in epinephrine secretion, glucocorticoid secretion, sex behavior, and food intake.
Related Products: Anti-DBH-SAP (Cat. #IT-03)
Exaggerated postnatal surge of orexin and the effects of elimination of excess orexin on blood pressure in spontaneously hypertensive rats in postnatal development
Barnett S, Dong R, Briggs L, Moushey A, Li A (2020) Exaggerated postnatal surge of orexin and the effects of elimination of excess orexin on blood pressure in spontaneously hypertensive rats in postnatal development. bioRxiv 2020.06.17.158279. doi: 10.1101/2020.06.17.158279
Related Products: Orexin-B-SAP (Cat. #IT-20)
Central circuit mechanisms of itch.
Chen XJ, Sun YG (2020) Central circuit mechanisms of itch. Nat Commun 11:3052. doi: 10.1038/s41467-020-16859-5
Summary: The authors summarize the progress in elucidating the neural circuit mechanism of itch at spinal and supraspinal levels.
Related Products: SSP-SAP (Cat. #IT-11)