References

Feng X, Guo Q, Xue H, Duan X, Jin S, Wu Y (2020) Hydrogen sulfide attenuated Angiotensin II-induced sympathetic excitation in offspring of renovascular hypertensive rats. Front Pharmacol 11:565726. doi: 10.3389/fphar.2020.565726 PMID: 33041805

Objective: To whether sensitivity to angiotensin II (Ang II) is enhanced in offspring of renovascular hypertensive animals and whether hydrogen sulfide (H2S) can attenuate the increased response to Ang II in offspring.

Summary: Prenatal or postnatal administration of H2S attenuated AngII-induced sympathetic excitation in offspring of hypertensive rats.

Usage: Western blot (1:800)

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Bumanglag AV, Truckenbrod LM, Chun E, Hernandez AR, Federico QP, Maurer AP, Sloviter RS, Burke SN (2019) Featured Article: Targeted hippocampal GABA neuron ablation produces hippocampal sclerosis, epilepsy, and dissociable effects on the Morris water maze and object-place paired association tasks. Targeting Trends 20

Related Products: SSP-SAP (Cat. #IT-11)

Read the featured article in Targeting Trends.

See Also:

• Chun E et al. Targeted hippocampal GABA neuron ablation by stable substance P-saporin causes hippocampal sclerosis and chronic epilepsy in rats. Epilepsia 60(5):e52-e57, 2019.

Krieger JP (2020) Intestinal glucagon-like peptide-1 effects on food intake: Physiological relevance and emerging mechanisms. Peptides 131:170342. doi: 10.1016/j.peptides.2020.170342 PMID: 32522585

Objective: Review of findings of GLP-1 involvement in obesity.

Summary: The most selective method of gastrointestinal vagal deafferentation to date used CCK-SAP injected into the nodose ganglia which blunts the anorexigenic effect of IP GLP-1 administration.

Related Products: CCK-SAP (Cat. #IT-31)

See Also:

• Diepenbroek C et al. Validation and characterization of a novel method for selective vagal deafferentation of the gut. Am J Physiol Gastrointest Liver Physiol 313:G342-G352, 2017.

Bolshakov AP, Stepanichev MY, Dobryakova YV, Spivak, YS, Markevich, VA (2020) Saporin from Saponaria officinalis as a tool for experimental research, modeling, and therapy in neuroscience. Toxins (Basel) 12(9):546. doi: 10.3390/toxins12090546

Summary: A review of studies where saporin-based conjugates were used to analyze cell mechanisms of sleep, general anesthesia, epilepsy, pain, and development of Parkinson’s and Alzheimer’s diseases.

Related Products: Targeted Toxins

Chew C, Sengelaub DR (2020) Exercise promotes recovery after motoneuron injury via hormonal mechanisms. Neural Regen Res 15(8):1373-1376. doi: 10.4103/1673-5374.274323

Objective: To describe how exercise is neuroprotective for motoneurons, accelerating axon regeneration following axotomy and attenuating dendritic atrophy following the death of neighboring motoneurons.

Summary: Exercise offers a simple, low barrier-to-entry behavioral intervention which is neuroprotective and pro-regenerative following neural injury.

Usage: Motoneurons innervating the left vastus medialis muscle were selectively killed by intramuscular injection of CTB-SAP (2 μL, 0.1%).

Related Products: CTB-SAP (Cat. #IT-14)

Lee SB, Ko A, Oh YT, Shi P, D’Angelo F, Frangaj B, Koller A, Chen EI, Cardozo T, Iavarone A, Lasorella A (2020) Proline hydroxylation primes protein kinases for autophosphorylation and activation. Mol Cell 79(3):376-389.e8. doi: 10.1016/j.molcel.2020.06.021 PMID: 32640193

Objective: To investigate the formation of prolyl-hydroxylated intermediates as a novel mechanism of kinase maturation and a general mechanism of regulation of CMGC kinases in eukaryotes.

Summary: Identified a highly conserved proline in the kinase domain of CMGC kinases that is hydroxylated by the PHD1 prolyl-hydroxylase, and this event precedes tyrosine autophosphorylation.

Usage: Western blot

Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)

Kucharczyk MW, Chisholm KI, Denk F, Dickenson AH, Bannister K, McMahon SB (2020) The impact of bone cancer on the peripheral encoding of mechanical pressure stimuli. Pain 161(8):1894-1905. doi: 10.1097/j.pain.0000000000001880 PMID: 32701848

Usage: immunohistochemistry (1:400)

Related Products: trkA Rabbit Polyclonal (Cat. #AB-N03)

Knuplez E, Krier-Burris R, Cao Y, Marsche G, O’Sullivan J, Bochner BS (2020) Superior mouse eosinophil depletion in vivo targeting transgenic Siglec-8 instead of endogenous Siglec-F: Mechanisms and pitfalls. J Leukoc Biol 108:43-58. doi: 10.1002/jlb.3hi0120-381r

Objective: To determine if targeting Siglec-8 with mouse IgG1 antibodies, rather than targeting Siglec-F with rat IgG antibodies, in mice transgenic for Siglec-8, will prove to be a more effective strategy for eliminating mouse eosinophils in vivo.

Summary: This study is the first to describe a novel mouse strain of Siglec-8+F− eosinophils—a useful tool for studying human Siglec biology in preclinical models.

Usage: Siglec-8+F− mouse eosinophils were pretreated with 10 μg/mL saporin-conjugated isotype controls (mouse IgG1 or rat IgG2), anti-Siglec-8 (2C4) or anti-Siglec-F (9C7) antibodies for 24 h.

Related Products: Custom Conjugates

Zhao L, Wang J, Zhang Y, Wang L, Yu M, Wang F (2020) Vitamin C decreases VEGF expression levels via hypoxia‑inducible factor‑1α dependent and independent pathways in lens epithelial cells. Mol Med Rep 22(1):436-444. doi: 10.3892/mmr.2020.11103 PMID: 32377733

Objective: To investigate the mechanisms underlying the effects of vitamin C on the expression levels of VEGF.

Summary: Vitamin C inhibits the cell proliferation and the expression levels of VEGF in lens epithelial cells following a co-treatment with the HIF-1 inhibitor.

Usage: Western blot (1:500)

Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)

da Rocha ES, Gomes FC, de Oliveira MR, Bruneto RV, Borges RMR, de Carvalho EP, Angeli-Junior EV, Garcia-Filho GF, Doná JPL, de Oliveira Batanero RP, Carlos CP, de Melo-Neto JS (2020) Effects of adjuvant-induced arthritis on the ventral prostate of rats treated with angiotensin AT1 receptor blocker. Life Sci 252:117629. doi: 10.1016/j.lfs.2020.117629 PMID: 32247619

Objective: To analyze the prostatic compartments, extracellular matrix, microvascularization, transforming growth factor-beta (TGF-β) and angiotensin II receptors type 1 (AT1) levels, and histopathology of the ventral prostate in a rat model for rheumatoid arthritis, and to evaluate the effect of angiotensin II AT1 receptor blocker (ARB) in the disease.

Summary: AIA may activate specific mechanisms that modify the prostatic environment; the use of ARB attenuates some altered prostate parameters in a rat model for arthritis.

Usage: Immunohistochemistry (1:500)

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)