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Proline hydroxylation primes protein kinases for autophosphorylation and activation

Lee SB, Ko A, Oh YT, Shi P, D’Angelo F, Frangaj B, Koller A, Chen EI, Cardozo T, Iavarone A, Lasorella A (2020) Proline hydroxylation primes protein kinases for autophosphorylation and activation. Mol Cell 79(3):376-389.e8. doi: 10.1016/j.molcel.2020.06.021

Objective: To investigate the formation of prolyl-hydroxylated intermediates as a novel mechanism of kinase maturation and a general mechanism of regulation of CMGC kinases in eukaryotes.

Summary: Identified a highly conserved proline in the kinase domain of CMGC kinases that is hydroxylated by the PHD1 prolyl-hydroxylase, and this event precedes tyrosine autophosphorylation.

Usage: Western blot

Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044A)