Lee SB, Ko A, Oh YT, Shi P, D’Angelo F, Frangaj B, Koller A, Chen EI, Cardozo T, Iavarone A, Lasorella A (2020) Proline hydroxylation primes protein kinases for autophosphorylation and activation. Mol Cell 79(3):376-389.e8. doi: 10.1016/j.molcel.2020.06.021
Objective: To investigate the formation of prolyl-hydroxylated intermediates as a novel mechanism of kinase maturation and a general mechanism of regulation of CMGC kinases in eukaryotes.
Summary: Identified a highly conserved proline in the kinase domain of CMGC kinases that is hydroxylated by the PHD1 prolyl-hydroxylase, and this event precedes tyrosine autophosphorylation.
Usage: Western blot
Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044A)