1. Home
  2. Knowledge Base
  3. 2020 Targeting Trends Review

2020 Targeting Trends Review

54 entries

Intracellular delivery methods using biofunctional peptide-modified extracellular vesicles

Nakase I (2020) Intracellular delivery methods using biofunctional peptide-modified extracellular vesicles. Extracell Vesicles Circ Nucleic Acids 1:44. American Society for Exosomes and Microvesicles 2020 Annual Meeting. doi: 10.20517/evcna.2020.10

Related Products: Saporin (Cat. #PR-01)

Obesity shapes metabolism in the tumor microenvironment to suppress anti-tumor immunity

Ringel AE, Drijvers JM, Baker GJ, Catozzi A, García-Cañaveras JC, Gassaway BM, Miller BC, Juneja VR, Nguyen TH, Joshi S, Yao CH, Yoon H, Sage PT, LaFleur MW, Trombley JD, Jacobson CA, Maliga Z, Gygi SP, Sorger PK, Rabinowitz JD, Sharpe AH, Haigis MC (2020) Obesity shapes metabolism in the tumor microenvironment to suppress anti-tumor immunity. Cell 183(7):1848-1866.e26. doi: 10.1016/j.cell.2020.11.009 PMID: 33301708

Objective: To investigate how obesity shifts the metabolic landscape of the tumor microenvironment (TME) to inhibit T cell function and promote tumor growth.

Summary: High-fat diet – induced obesity impairs CD8+ T cell function in the murine TME, accelerating tumor growth.

Usage: Immunoprecipitation

Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)

Extracellular levels of the sleep homeostasis mediator, adenosine, are regulated by glutamatergic neurons during wakefulness and sleep

Sun MJ, Tang Y (2020) Extracellular levels of the sleep homeostasis mediator, adenosine, are regulated by glutamatergic neurons during wakefulness and sleep. Purinergic Signal 16(4):475-476. doi: 10.1007/s11302-020-09758-3

Summary: Blanco-Centurion et al. investigated the role of cholinergic neurons in the BF by administering 192–IgG–SAP to lesion them, but surprisingly the results indicated that adenosine from cholinergic neurons in BF are not essential to sleep induction.

See: Blanco-Centurion C et al. Adenosine and sleep homeostasis in the Basal forebrain. J Neurosci 26(31):8092-8100, 2006.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Differential mechanisms are required for phrenic long-term facilitation over the course of motor neuron loss following CTB-SAP intrapleural injections.

Borkowski LF, Nichols NL (2020) Differential mechanisms are required for phrenic long-term facilitation over the course of motor neuron loss following CTB-SAP intrapleural injections. Exp Neurol 334:113460. doi: 10.1016/j.expneurol.2020.113460

Objective: To understand the mechanism responsible for this difference in magnitude of phrenic long-term facilitation (pLTF)

Summary: pLTF in 7d CTB-SAP treated rats is elicited primarily through TrkB and PI3K/Akt-dependent mechanisms, whereas BDNF and MEK/ERK-dependent mechanisms induce pLTF in 28d CTB-SAP treated rats.

Usage: Rats received bilateral intrapleural injections of CTB-SAP; 25 μg dissolved in PBS.

Related Products: CTB-SAP (Cat. #IT-14)

Measuring attention in rats with a visual signal detection task: Signal intensity vs. signal duration

Holloway Z, Koburov R, Hawkey A, Levin ED (2020) Measuring attention in rats with a visual signal detection task: Signal intensity vs. signal duration. Pharmacol Biochem Behav 199:173069. doi: 10.1016/j.pbb.2020.173069

Summary: Drug-induced effects have been used to demonstrate the construct validity of operant attention tasks, as well as to assess the pharmacological systems that underlie cognitive processes, such as attention, short-term memory and reaction time, either by interrupting or enhancing performance.

See: McGaughy J et al. Sustained attention performance in rats with intracortical infusions of 192 IgG-saporin-induced cortical cholinergic deafferentation: effects of physostigmine and FG 7142. Behav Neurosci 112(6):1519-1525, 1998.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Depletion of C1 neurons attenuates the salt-induced hypertension in unanesthetized rats.

Ribeiro N, Martins Sá RW, Antunes VR (2020) Depletion of C1 neurons attenuates the salt-induced hypertension in unanesthetized rats. Brain Res 1748:147107. doi: 10.1016/j.brainres.2020.147107

Objective: To determine if the ablation of C1 neurons mitigates high blood pressure induced by high-salt intake.

Summary: Data show that hypertension induced by high-salt intake is dependent on C1 neurons.

Usage: Bilateral injections of 2.4 ng/100 nl of Anti-DBH-SAP. The total number of TH+ neurons in the AS region was reduced by 37 ± 13% in the anti-DBH-SAP group when compared to control.

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Pseudomonas Exotoxin A based toxins targeting epidermal growth factor receptor for the treatment of prostate cancer

Fischer A, Wolf I, Fuchs H, Masilamani AP, Wolf P (2020) Pseudomonas Exotoxin A based toxins targeting epidermal growth factor receptor for the treatment of prostate cancer. Toxins (Basel) 12(12):753. doi: 10.3390/toxins12120753

Summary: Refers to chimeric murine-human mAb cetuximab bound to Streptavidin-ZAP.

See: Yip WL et al. Targeted Delivery and Enhanced Cytotoxicity of Cetuximab-Saporin by Photochemical Internalization in EGFR-Positive Cancer Cells. Mol Pharm 4(2):241-251, 2007.

Related Products: Streptavidin-ZAP (Cat. #IT-27)

Reversal of object recognition memory deficit in perirhinal cortex-lesioned rats and primates and in rodent models of aging and alzheimer’s diseases.

Masmudi-Martín M, Navarro-Lobato I, López-Aranda MF, Browning PGF, Simón A-M, López-Téllez JF, Jiménez-Recuerda I, Martîn-Montañez E, Pérez-Mediavilla A, Frechilla D, Baxter MG, Khan ZU (2020) Reversal of object recognition memory deficit in perirhinal cortex-lesioned rats and primates and in rodent models of aging and alzheimer’s diseases. Neuroscience 448:287-298. doi: 10.1016/j.neuroscience.2020.08.039

Objective: To determine if Object Recognition Memory (ORM) can be restored.

Summary: Memory-deficient rats were generated by induction of lesions to the perirhinal cortex (PRh) through an injection of OX7-SAP. Expression of regulator of G-protein signaling 14 of 414 amino acids (RGS14414) restored ORM in memory-deficient PRh-lesioned rats and nonhuman primates. This treatment was sufficient for full recovery of ORM in rodent models of aging and Alzheimer’s disease.

Usage: Rats were injected with OX7-SAP (0.9 mg in 1ml) in the PRh of the brain.

Related Products: OX7-SAP (Cat. #IT-02)

Loss of diffuse noxious inhibitory control after traumatic brain injury in rats: A chronic issue

Irvine KA, Sahbaie P, Ferguson AR, Clark JD (2020) Loss of diffuse noxious inhibitory control after traumatic brain injury in rats: A chronic issue. Exp Neurol 333:113428. doi: 10.1016/j.expneurol.2020.113428

Objective: To confirm hypothesis that dysfunctional descending noradrenergic and serotonergic pain control circuits may contribute to the loss of diffuse noxious inhibitory control (DNIC), a critical endogenous pain control mechanism, weeks to months after traumatic brain injury (TBI).

Summary: Results suggest that TBI causes maladaptation of descending nociceptive signaling mechanisms and changes in the function of both adrenergic and serotonergic circuits. Such changes could predispose those with TBI to chronic pain.

Usage: Anti-DBH-SAP (5 μg/5 μl) was injected in the left ventricle. Lesion of the LC resulted in failure of DNIC, an effect that mimics what is observed behaviorally after chronic TBI.

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Noradrenaline signaling in the LPBN mediates amylin’s and salmon calcitonin’s hypophagic effect in male rats.

Boccia L, Le Foll C, Lutz TA (2020) Noradrenaline signaling in the LPBN mediates amylin’s and salmon calcitonin’s hypophagic effect in male rats. FASEB J 34(11):15448-15461. doi: 10.1096/fj.202001456RRR

Objective: To assess the phenotype of amylin activated LPBN (lateral parabrachial nucleus) neurons, especially to confirm the CGRPergic phenotype and to uncover the specific role of NA (noradrenaline) signaling from the AP to the LPBN.

Summary: The present study confirmed the central role of the LPBN in propagating amylin’s and sCT’s hypophagic action, and particularly the importance of AP → LPBN NA signaling in the mediation of this process, through the activation of LPBN (CGRP and non-CGRP) neurons.

Usage: The neuronal pathways used to process the physiological response to amylin were investigated using 50-ng injections of Anti-DBH-SAP (Cat. #IT-03) into the area postrema (AP) or 25 ng into the lateral parabrachial nucleus (Potes et al., 2010).

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Shopping Cart
Scroll to Top