References

Gu Z, Yu J, Werrbach-Perez K, Perez-Polo JR (2000) Repeated immunolesions display diminished stress response signal. Int J Dev Neurosci 18:177-183. doi: 10.1016/s0736-5748(99)00086-6

Summary: Cholinergic neurons in the basal forebrain are involved in neurotrophin release in general injury response although this response is impaired in the aged individual. Addition of pharmacological doses of NGF can repair this mechanism. Gu et al. used 192-Saporin (Cat. #IT-01) to model the endogenous stimulation of NGF in response to injury. They found that a one-time administration of 192-Saporin was more effective than chronic repeated administrations for inducing an increase in NGF levels. These results indicate that chronic lesions may cause a desensitization that differs from the acute toxic model.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Rossner S, Schliebs R, Bigl V (2000) Intracerebroventricular infusion of CHO5, a rat monoclonal antibody directed against mouse low-affinity nerve growth factor receptor (p75NTR), specifically labels basal forebrain cholinergic neurons in mouse brain. Metab Brain Dis 15(1):17-27. doi: 10.1007/BF02680011 PMID: 10885538

Summary: 192-Saporin (Cat. #IT-01) has long been a useful tool for neurobiological research in the rat. For various reasons, many researchers want to perform the same studies in the mouse but have been prevented from doing so by the lack of a suitable antibody against the mouse p75NTR. Rossner et al. describe a rat monoclonal antibody against the mouse p75NTR (Cat. #AB-N02) that demonstrates co-localization of p75NTR and ChAT, and also co-localization of p75NTR and TrkA in the mouse basal forebrain. Internalization and retrograde transport of this antibody in cholinergic basal forebrain neurons is also shown. This evidence indicates that the anti-mouse p75NTR will be effective for use as an immunotoxin.

Related Products: 192-IgG-SAP (Cat. #IT-01), mu p75-SAP (Cat. #IT-16), Antibody to NGFR (Cat. #AB-N02)

Wirth S, Lehmann O, Bertrans F, Lazarus C, Jeltsch H, Cassel JC (2000) Preserved olfactory short-term memory after combined cholinergic and serotonergic lesions using 192 IgG-Saporin and 5,7-dihydroxytryptamine in rats [published erratum appears in Neuroreport 2000 Mar 20;11(4):inside back cover]. Neuroreport 11:347-350. doi: 10.1097/00001756-200002070-00025

Usage: 192-SAP (Cat. #IT-01) 2 µg, intracerebroventricular

Related Products: 192-IgG-SAP (Cat. #IT-01)

Kitahama K, Nagatsu I, Geffard M, Maeda T (2000) Distribution of dopamine-immunoreactive fibers in the rat brainstem. . J Chem Neuroanat 18(1-2):1-9. doi: 10.1016/s0891-0618(99)00047-2 PMID: 10708914

Related Products: Dopamine Mouse Monoclonal, Conjugated (Cat. #AB-T11)

Reyes-Reyna SM, Krolick KA (2000) Chemokine production by rat myocytes exposed to interferon-gamma. Clin Immunol 94(2):105-113. doi: 10.1006/clim.1999.4828 PMID: 10637095

Related Products: Nicotinic Acetylcholine Receptor alpha 1 (mAb 35) Rat Monoclonal (Cat. #AB-N36)

Maciejewski-Lenoir D, Heinrichs SC, Liu X-J, Ling N, Tucker A, Xie Q, Lappi DA, Grigoriadis DE (2000) Selective impairment of corticotropin-releasing factor1 (CRF1) receptor-mediated function using CRF coupled to saporin. Endocrinol 141:498-504. doi: 10.1210/endo.141.2.7336

Summary: Corticotropin-releasing factor 1 (CRF1) is a 41-amino acid peptide which mediates many of the body’s behavioral, autonomic, immune, and endocrine responses to stress. Reduced activation of the CRF systems plays a role in a variety of psychiatric and metabolic disease states. Maciejewski-Lenoir et al. have developed a CRF-SAP targeted toxin that can eliminate cells expressing the CRF1 but not CRF2a receptors. These data indicate that CRF-SAP (Cat. #IT-13) may be useful as a tool to examine receptor-selective impairment of CRF system function.

Related Products: CRF-SAP (Cat. #IT-13)

Fabbrini MS, Carpani D, Soria MR, Ceriotti A (2000) Cytosolic immunization allows the expression of preATF-saporin chimeric toxin in eukaryotic cells. FASEB J 14(2):391-398. doi: 10.1096/fasebj.14.2.391 PMID: 10657995

Related Products: Saporin Goat Polyclonal (Cat. #AB-15)

Steggerda SM, Black BE, Paschal BM (2000) Monoclonal antibodies to NTF2 inhibit nuclear protein import by preventing nuclear translocation of the GTPase Ran. Mol Biol Cell 11(2):703-719. doi: 10.1091/mbc.11.2.703 PMID: 10679025

Related Products: Antibody to NTF2 (5A3) (Cat. #AB-V66), Antibody to NTF2 (4F5) (Cat. #AB-V67), Antibody to NTF2 (5E8) (Cat. #AB-V68)

Winkler J, Ramirez GA, Thal LJ, Waite JJ (2000) Nerve growth factor (NGF) augments cortical and hippocampal cholinergic functioning after p75NGF receptor-mediated deafferentation but impairs inhibitory avoidance and induces fear-related behaviors. J Neurosci 20:834-844. doi: 10.1523/JNEUROSCI.20-02-00834.2000

Usage: 192-SAP (Cat. #IT-01) 1.0 or 2.7 µg in 10 µl, intracerebroventricular

Related Products: 192-IgG-SAP (Cat. #IT-01)

Provencio I, Rodriguez IR, Jiang G, Hayes WP, Moreira EF, Rollag MD (2000) A novel human opsin in the inner retina. J Neurosci 20(2):600-605. doi: 10.1523/JNEUROSCI.20-02-00600.2000 PMID: 10632589

Summary: Provencio and colleagues found that melanopsin is also present in mouse retina, specifically in ganglion cells, and that it mediates non-visual photoreceptive tasks.

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38), Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)