Nucleocytoplasmic transport is essential for regulating processes such as transcription, translation, growth factor signaling, stress responses, and cell cycle progression. Nuclear transport factor 2 (NTF2) is a soluble transport protein first identified for its ability to promote nuclear localization signal (NLS)-dependent protein import in digitonin-permeabilized cells. NTF2 interacts with nuclear pore complex proteins and binds the GDP-bound form of Ran in vitro, facilitating the transport of cargo proteins into the nucleus.
