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Synergistic effects of unilateral immunolesions of the cholinergic projections from the basal forebrain and contralateral ablations of the inferotemporal cortex and hippocampus in monkeys.
Barefoot HC, Baker HF, Ridley RM (2000) Synergistic effects of unilateral immunolesions of the cholinergic projections from the basal forebrain and contralateral ablations of the inferotemporal cortex and hippocampus in monkeys. Neuroscience 98(2):243-251. doi: 10.1016/s0306-4522(00)00131-7
Related Products: ME20.4-SAP (Cat. #IT-15)
Immunohistochemical localization of protein 3-nitrotyrosine and S-nitrosocysteine in a murine model of inhaled nitric oxide therapy.
Lorch SA, Foust R 3rd, Gow A, Arkovitz M, Salzman AL, Szabo C, Vayert B, Geffard M, Ischiropoulos H (2000) Immunohistochemical localization of protein 3-nitrotyrosine and S-nitrosocysteine in a murine model of inhaled nitric oxide therapy. Pediatr Res 47(6):798-805. doi: 10.1203/00006450-200006000-00020 PMID: 10832741
Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)
NGF-mediated alteration of NF-κB binding activity after partial immunolesions to rat cholinergic basal forebrain neurons.
Gu Z, Toliver-Kinsky T, Glasgow J, Werrbach-Perez K, Perez-Polo JR (2000) NGF-mediated alteration of NF-κB binding activity after partial immunolesions to rat cholinergic basal forebrain neurons. Int J Dev Neurosci 18:455-468. doi: 10.1016/s0736-5748(00)00004-6
Summary: After injecting 1.3 µg 192-Saporin (Cat. #IT-01) into the lateral ventricle of rat brain, followed by infusion of NGF antibody, Gu et al. report changes in the activity of the transcription factor NF-B. Aged rodent brains show an increase in NF-kappaB activity. This model creates a tool to investigate decreased cholinergic function that is often associated with memory loss and cognitive deficits in the elderly and particularly in patients with Alzheimer’s disease.
Related Products: 192-IgG-SAP (Cat. #IT-01)
Cholinergic excitation of septohippocampal GABA but not cholinergic neurons: implications for learning and memory.
Wu M, Shanabrough M, Leranth C, Alreja M (2000) Cholinergic excitation of septohippocampal GABA but not cholinergic neurons: implications for learning and memory. J Neurosci 20(10):3900-3908. doi: 10.1523/JNEUROSCI.20-10-03900.2000 PMID: 10804229
Summary: It has long been assumed that the drug-induced enhancement of learning and memory in both young and aged rats was accomplished through a cholinergic pathway in the hippocampus. Wu et al. used a fluorescent labeling molecule, 192-IgG conjugated to Cy3 (Custom Service from ATS) to visualize these neurons. They found that the effects of cognition-enhancing drugs are not facilitated through action on cholinergic neurons. Instead, activation of GABA neurons is implicated in this model.
Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #AB-N43FL3)
Immunolocalization of the cocaine- and antidepressant-sensitive 1-norepinephrine transporter.
Schroeter S, Apparsundaram S, Wiley RG, Miner LH, Sesack SR, Blakely RD (2000) Immunolocalization of the cocaine- and antidepressant-sensitive 1-norepinephrine transporter. J Comp Neurol 420:211-232. doi: 10.1002/(SICI)1096-9861(20000501)420:2<211::AID-CNE5>3.0.CO;2-3
Summary: Norepinephrine transporters are involved in the response to multiple antidepressants and psychostimulants, but the expression of these proteins has not yet been characterized in the central nervous system. Schroeter et al. used an antibody to a cytoplasmic epitope of norepinephrine transporters to map the transporters to noradrenergic neuronal somata, axons, and dendrites. To verify the specificity of the antibody the researchers injected 10 µg of anti-DBH-SAP (Cat. #IT-03) in the left lateral ventricle of rats to destroy the noradrenergic neurons, confirming the specificity of the norepinephrine transporter antibody. Treatment with anti-DBH-SAP completely removed norepinephrine transporter immunoreactivity.
Related Products: Anti-DBH-SAP (Cat. #IT-03)
Cortical cholinergic denervation elicits vascular A beta deposition.
Roher AE, Kuo YM, Potter PE, Emmerling MR, Durham RA, Walker DG, Sue LI, Honer WG, Beach TG (2000) Cortical cholinergic denervation elicits vascular A beta deposition. Ann NY Acad Sci 903:366-373. doi: 10.1111/j.1749-6632.2000.tb06388.x
Related Products: ME20.4-SAP (Cat. #IT-15)
Impaired acquisition of a Morris water maze task following selective destruction of cerebellar Purkinje cells with OX7-saporin.
Gandhi CC, Kelly RM, Wiley RG, Walsh TJ (2000) Impaired acquisition of a Morris water maze task following selective destruction of cerebellar Purkinje cells with OX7-saporin. Behav Brain Res 109:37-47. doi: 10.1016/s0166-4328(99)00160-6
Summary: The cerebellum has been associated with the control of motor activity and voluntary movements. Recent data have shown the cerebellum may also play a role in “higher order” processes such as learning, language, and cognition. Using 2 µg OX7-SAP (Cat. #IT-02) by i.c.v. injection, Gandhi et al. selectively eliminated Purkinje cells in rat cerebellum in order to examine the ability of treated animals to complete a water maze task. Elimination of these cells significantly impaired the ability of the rats to complete the task, suggesting the cerebellum is involved in learning.
Related Products: OX7-SAP (Cat. #IT-02)
Cholinergic deafferentation of the rabbit cortex: a new animal model of Abeta deposition.
Beach TG, Potter PE, Kuo YM, Emmerling MR, Durham RA, Webster SD, Walker DG, Sue LI, Scott S, Layne KJ, Roher AE (2000) Cholinergic deafferentation of the rabbit cortex: a new animal model of Abeta deposition. Neurosci Lett 283:9-12. doi: 10.1016/s0304-3940(00)00916-2
Related Products: ME20.4-SAP (Cat. #IT-15)
Recombinant toxins that bind to the urokinase receptor are cytotoxic without requiring binding to the alpha(2)-macroglobulin receptor.
Rajagopal V, Kreitman RJ (2000) Recombinant toxins that bind to the urokinase receptor are cytotoxic without requiring binding to the alpha(2)-macroglobulin receptor. J Biol Chem 275(11):7566-7573. doi: 10.1074/jbc.275.11.7566
Related Products: Saporin (Cat. #PR-01)
Attenuation of the bidirectional effects of chlordiazepoxide and FG 7142 on conditioned response suppression and associated cardiovascular reactivity by loss of cortical cholinergic inputs.
Stowell JR, Berntson GG, Sarter M (2000) Attenuation of the bidirectional effects of chlordiazepoxide and FG 7142 on conditioned response suppression and associated cardiovascular reactivity by loss of cortical cholinergic inputs. Psychopharmacol 150(2):141-149. doi: 10.1007/s002130000443
Summary: The benzodiazepine receptor (BR) is involved in anxiety. It has been hypothesized that cholinergic projections from the CBF are necessary for modulation of the BR by agonists and inverse agonists. Stowell et al. directly injected 0.18 µg 192-Saporin (Cat #IT-01) into each hemisphere of the CBF in adult rats. The treated rats had altered responses to external stimuli during an operant conditioned task. These results indicate that the CBF plays an important role in response to fear and anxiety-related stimuli. This system may also mediate the actions of BR ligands.
Related Products: 192-IgG-SAP (Cat. #IT-01)