References

Blanco-Centurion C (2002) Featured Article: HCRT-SAP lesion produces sleepiness while anti-DBH-SAP lesion does not. Targeting Trends 3(2)

Related Products: Anti-DBH-SAP (Cat. #IT-03), Orexin-B-SAP (Cat. #IT-20)

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See Also:

• Blanco-Centurion CA et al. Hypocretin B-Saporin Lesions Of The Brainstem Increase Rem Sleep At Night. Neuroscience 2001 Abstracts 410.9, 2001. Society for Neuroscience, San Diego, CA

Butt AE, Noble MM, Rogers JL, Rea TE (2002) Impairments in negative patterning, but not simple discrimination learning, in rats with 192 IgG-Saporin lesions of the nucleus basalis magnocellularis. Behav Neurosci 116(2):241-255. doi: 10.1037//0735-7044.116.2.241

Summary: 192-Saporin (Cat. #IT-01) administration to the basal forebrain has frequently been used in rats to create a model for Alzheimer’s disease. The authors used 0.2 µl bilateral injections of 0.4 µg/µl 192-SAP into the nucleus basalis magnocellularis (NBM). Previous studies using non-specific excitotoxic agents have suggested the involvement of the NBM in learning and memory. The authors confirm more recent findings that indicate some of the deficits produced by these excitotoxins are due to the non-specific lesioning caused by these agents. The highly selective cholinergic lesioning produced by 192-Saporin left simple association learning intact but impaired more complicated configural association processes.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Butt AE, Bowman TD (2002) Transverse patterning reveals a dissociation of simple and configural association learning abilities in rats with 192 IgG-saporin lesions of the nucleus basalis magnocellularis. Neurobiol Learn Mem 77:211-233. doi: 10.1006/nlme.2001.4013

Summary: Using 80 ng bilateral infusions of 192-Saporin (Cat. #IT-01) into each of the medial and lateral target sites of the nucleus basalis magnocellularis (NBM) in rats, the authors demonstrate that lesioning the cholinergic systems of the NBM impairs a more complicated learning technique, while leaving simple association learning intact. The results also show that the transition between these two learning strategies is impaired in lesioned animals.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Mattsson A, Ögren SO, Olson L (2002) Facilitation of dopamine-mediated locomotor activity in adult rats following cholinergic denervation. Exp Neurol 174:96-108. doi: 10.1006/exnr.2001.7850

Summary: Using 80 ng bilateral infusions of 192-Saporin (Cat. #IT-01) into each of the medial and lateral target sites of the nucleus basalis magnocellularis (NBM) in rats, the authors demonstrate that lesioning the cholinergic systems of the NBM impairs a more complicated learning technique, while leaving simple association learning intact. The results also show that the transition between these two learning strategies is impaired in lesioned animals.

Related Products: 192-IgG-SAP (Cat. #IT-01)

McGaughy J, Dalley JW, Morrison CH, Everitt BJ, Robbins TW (2002) Selective behavioral and neurochemical effects of cholinergic lesions produced by intrabasalis infusions of 192 IgG-saporin on attentional performance in a five-choice serial reaction time task. J Neurosci 22(5):1905-1913. doi: 10.1523/JNEUROSCI.22-05-01905.2002

Summary: 192-Saporin (Cat. #IT-01) has been a very useful tool in determining the role of the basal forebrain cholinergic system in arousal and attention tasks. The authors lesioned the nucleus basalis magnocellularis of rats with an infusion of 0.5 µl per hemisphere of 0.15 µg/µl or 0.45 µg/µl 192-Saporin. The data show a correlation between the extent of the lesion and the amount of impairment in an attentional task. The authors also found that the accuracy deficits in the task could be ameliorated by lengthening the stimulus time, or exacerbated by increasing the event rate. Taken together the data indicate a direct relationship between basal forebrain damage and impaired attentional function.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Power AE, Thal LJ, McGaugh JL (2002) Lesions of the nucleus basalis magnocellularis induced by 192 IgG-saporin block memory enhancement with posttraining norepinephrine in the basolateral amygdala. Proc Natl Acad Sci U S A 99(4):2315-2319. doi: 10.1073/pnas.022627799

Summary: There is evidence that memory consolidation (retention) can be modulated by drugs and stress hormones acting in the basolateral amygdala (BLA). The BLA sends projections to the nucleus basalis magnocellularis (NBM), which in turn sends cholinergic projections to the neocortex. The authors used 100 ng bilateral infusions of 192-Saporin (Cat. #IT-01) in 500 nl 0.1 M PBS to investigate whether lesions of the cholinergic NBM projections affect BLA modulation of memory. 192-Saporin lesions blocked memory enhancement normally induced by norepinephrine infusions into the BLA. This finding suggests NBM-cortex projections may mediate BLA modulation of memory storage or processing in the neocortex.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Lembo PM, Grazzini E, Groblewski T, O’Donnell D, Roy MO, Zhang J, Hoffert C, Cao J, Schmidt R, Pelletier M, Labarre M, Gosselin M, Fortin Y, Banville D, Shen SH, Strom P, Payza K, Dray A, Walker P, Ahmad S (2002) Proenkephalin A gene products activate a new family of sensory neuron-specific GPCRs. Nat Neurosci 5(3):201-209. doi: 10.1038/nn815 PMID: 11850634

Related Products: BAM22P Rabbit Polyclonal (Cat. #AB-12)

Kingery WS, Agashe GS, Guo TZ, Sawamura S, Davies MF, Clark JD, Kobilka BK, Maze M (2002) Isoflurane and nociception: Spinal alpha2A adrenoceptors mediate antinociception while supraspinal alpha1 adrenoceptors mediate pronociception. Anesthesiol 96:367-374. doi: 10.1097/00000542-200202000-00023

Summary: The authors injected 3 µg/3 µl of anti-DBH-SAP (Cat. #IT-03) into the lateral ventricle of rats to determine whether noradrenergic brainstem nuclei and descending spinal pathways are responsible for the antinociceptive actions of isoflurane. The results indicate that isoflurane modulates nociception by as many as three mechanisms, utilizing various combinations of noradrenergic neurons, adrenoceptors, and descending spinal pathways.

Related Products: Anti-DBH-SAP (Cat. #IT-03)

Provencio I, Rollag MD, Castrucci AM (2002) Photoreceptive net in the mammalian retina. Nature 415:493. doi: 10.1038/415493a PMID: 11823848

Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)

Lappi DA (2002) Featured Article: Control conjugates: The perfect companion for targeted toxins. Targeting Trends 3(1)

Related Products: Rat IgG-SAP (Cat. #IT-17), Mouse IgG-SAP (Cat. #IT-18), Goat IgG-SAP (Cat. #IT-19), Blank-SAP (Cat. #IT-21)

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