- Home
- Knowledge Base
- References
References
Oxaliplatin acts on IB4-positive nociceptors to induce an oxidative stress-dependent acute painful peripheral neuropathy.
Joseph EK, Chen X, Bogen O, Levine JD (2008) Oxaliplatin acts on IB4-positive nociceptors to induce an oxidative stress-dependent acute painful peripheral neuropathy. J Pain 9:463-472. doi: 10.1016/j.jpain.2008.01.335
Summary: Oxaliplatin is a platinum-based chemotherapy agent. Use of this reagent produces various pathological pain states, depending on the dosage site. The authors administered 3.2-µg intrathecal injections of IB4-SAP (Cat. #IT-10), using saporin (Cat. #PR-01) as a control. Lesioning IB4-binding neurons in the dorsal horn completely prevented oxaliplatin-induced hyperalgesia, indicating that the IB4-positive nociceptor neuronal subset is crucial to this type of neuropathy.
Related Products: Saporin (Cat. #PR-01), IB4-SAP (Cat. #IT-10)
Cholinergic deafferentation of prefrontal cortex increases sensitivity to cross-modal distractors during a sustained attention task.
Newman LA, McGaughy J (2008) Cholinergic deafferentation of prefrontal cortex increases sensitivity to cross-modal distractors during a sustained attention task. J Neurosci 28:2642-2650. doi: 10.1523/JNEUROSCI.5112-07.2008
Summary: The authors injected 5 ng of 192-IgG-SAP (Cat. #IT-01) into the prefrontal cortex of rats to investigate the effect of cholinergic loss on distractors to attentional demand. Where all animals experienced impaired performance in the presence of visual distractions, lesioned animals were more sensitive to auditory distractions. While these results indicate compromised top-down processing, lesioned animals showed improved performance in bottom-up processing, possibly caused by a shift in circuit dynamics after the lesion.
Related Products: 192-IgG-SAP (Cat. #IT-01)
Neuroanatomical and behavioral effects of a novel version of the cholinergic immunotoxin mu p75-saporin in mice.
Moreau PH, Cosquer B, Jeltsch H, Cassel JC, Mathis C (2008) Neuroanatomical and behavioral effects of a novel version of the cholinergic immunotoxin mu p75-saporin in mice. Hippocampus 18(6):610-622. doi: 10.1002/hipo.20422
Summary: 192-IgG-SAP (Cat. #IT-01) has been used for over a decade to examine the cholinergic system in the basal forebrain of rats. Establishing the same reagent for mice has been problematic. Here the authors describe the use of a mouse-specific lesioning agent, mu p75-SAP (Cat. #IT-16). After deciding on a dosage of 0.4 µg administered in the form of bilateral intracerebroventricular injections, mice were lesioned and tested. Lesioned animals displayed increased locomotor activity, and spatial learning and memory deficits, with minimal side effects.
Related Products: mu p75-SAP (Cat. #IT-16), 192-IgG-SAP (Cat. #IT-01)
Selective impairment of the cerebellar C1 module involved in rat hind limb control reduces step-dependent modulation of cutaneous reflexes.
Pijpers A, Winkelman BH, Bronsing R, Ruigrok TJ (2008) Selective impairment of the cerebellar C1 module involved in rat hind limb control reduces step-dependent modulation of cutaneous reflexes. J Neurosci 28:2179-2189. doi: 10.1523/JNEUROSCI.4668-07.2008
Summary: The cerebellar cortex is arranged in a series of modules. Elucidation of module-specific function has been difficult because of the closely arranged structure of these modules. Here the authors lesioned the C1/C3 hindlimb module of the rat with CTB-SAP (Cat. #IT-14). Rats received 75-125 ng injections of CTB-SAP into the C1 zone of the copula pyramidis or the paramedian lobule of the right cerebellar hemisphere. C1-injected animals displayed marked diminishment of cutaneously induced reflexes with no significant impact on walking or stepping pattern.
Related Products: CTB-SAP (Cat. #IT-14)
Lesions of the basal forebrain impair reversal learning but not shifting of attentional set in rats.
Tait DS, Brown VJ (2008) Lesions of the basal forebrain impair reversal learning but not shifting of attentional set in rats. Behav Brain Res 187:100-108. doi: 10.1016/j.bbr.2007.08.035
Summary: The authors compared specific lesions of the basal forebrain using 192-IgG-SAP (Cat. #IT-01) with non-specific lesions generated by ibotenic acid. Rats were given 0.12 µg per 0.5 µl bilateral injections of 192-IgG-SAP. The treated animals were then tested in food reward tasks involving two-choice discriminations and reversal of stimulus-reward. Animals with specific lesions did not show impairment with any of the tasks suggesting that non-cholinergic neurons are involved in reversal learning. This work also demonstrates the similarities between monkey and rodent basal forebrain function.
Related Products: 192-IgG-SAP (Cat. #IT-01)
Septal grafts restore cognitive abilities and amyloid precursor protein metabolism.
Aztiria E, Cataudella T, Spampinato S, Leanza G (2009) Septal grafts restore cognitive abilities and amyloid precursor protein metabolism. Neurobiol Aging 30(10):1614-1625. doi: 10.1016/j.neurobiolaging.2007.12.018
Summary: Although cholinergic loss and the presence of ß-amyloid plaques are well documented in Alzheimer’s disease, it is unknown whether restoration of regulatory cholinergic inputs affects in vivo b-amyloid precursor protein (APP). 5 µg of 192-IgG-SAP (Cat. #IT-01) was split between the lateral ventricles of rats. 6 months post-surgery the animals were implanted with cholinergic-rich septal tissue grafts. Grafted animals exhibited normal or near-normal levels of APP. APP levels, as well as improved spatial navigation performance, correlated strongly with graft-induced cholinergic changes.
Related Products: 192-IgG-SAP (Cat. #IT-01)
Chronic exposure to nerve growth factor increases acetylcholine and glutamate release from cholinergic neurons of the rat medial septum and diagonal band of Broca via mechanisms mediated by p75NTR.
Huh CY, Danik M, Manseau F, Trudeau LE, Williams S (2008) Chronic exposure to nerve growth factor increases acetylcholine and glutamate release from cholinergic neurons of the rat medial septum and diagonal band of Broca via mechanisms mediated by p75NTR. J Neurosci 28(6):1404-1409. doi: 10.1523/JNEUROSCI.4851-07.2008 PMID: 18256260
Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #AB-N43FL3)
Cholera toxin up-regulates endoplasmic reticulum proteins that correlate with sensitivity to the toxin
Dixit G, Mikoryak C, Hayslett T, Bhat A, Draper RK (2008) Cholera toxin up-regulates endoplasmic reticulum proteins that correlate with sensitivity to the toxin. Exp Biol Med (Maywood) 233(2):163-175. doi: 10.3181/0705-RM-132 PMID: 18222971
Related Products: Cholera Toxin subunit A Rabbit Polyclonal (Cat. #AB-43)
Expression of osteopontin in the rat retina: effects of excitotoxic and ischemic injuries.
Chidlow G, Wood JP, Manavis J, Osborne NN, Casson RJ (2008) Expression of osteopontin in the rat retina: effects of excitotoxic and ischemic injuries. Invest Ophthalmol Vis Sci 49(2):762-771. doi: 10.1167/iovs.07-0726 PMID: 18235026
Related Products: Antibody to OX7 (Cat. #AB-N08)
The pedunculopontine tegmental nucleus and the nucleus basalis magnocellularis: do both have a role in sustained attention?
Rostron CL, Farquhar MJ, Latimer MP, Winn P (2008) The pedunculopontine tegmental nucleus and the nucleus basalis magnocellularis: do both have a role in sustained attention?. BMC Neurosci 9:16. doi: 10.1186/1471-2202-9-16
Summary: This study provided further investigation into the role of the pedunculopontine tegmental nucleus (PPTg) in control of sustained attention. Rats were given 0.13 µg injections of 192-IgG-SAP (Cat. #IT-01) into the nucleus basalis magnocellularis. The immunotoxin-treated animals were compared to animals receiving ibotenate injections into the PPTg. Results suggest that ibotenate lesions cause impaired selection of conditioned response as shown by an increase in unconditioned behaviors. 192-IgG-SAP treated animals exhibited difficulty obtaining successful lever presses linked to attention.
Related Products: 192-IgG-SAP (Cat. #IT-01)