References

Beaulieu JM, Zhang X, Rodriguiz RM, Sotnikova TD, Cools MJ, Wetsel WC, Gainetdinov RR, Caron MG (2008) Role of GSK3 beta in behavioral abnormalities induced by serotonin deficiency. Proc Natl Acad Sci U S A 105(4):1333-1338. doi: 10.1073/pnas.0711496105 PMID: 18212115

Related Products: Antibody to Serotonin Transporter (SERT, Cat. #AB-N09)

Andäng M, Hjerling-Leffler J, Moliner A, Lundgren TK, Castelo-Branco G, Nanou E, Pozas E, Bryja V, Halliez S, Nishimaru H, Wilbertz J, Arenas E, Koltzenburg M, Charnay P, Manira AE, Ibañez CF, Ernfors P (2008) Histone H2AX-dependent GABAA receptor regulation of stem cell proliferation. Nature 451(7177):460-464. doi: 10.1038/nature06488 PMID: 18185516

Summary: Immunofluorescence staining for vesicular GABA transporter (VGAT) in embryonic stem cells reveals a punctate localization.

Usage: Immunostaining and western blotting. After being blocked for 2 h in PBS containing 3% BSA and 0.3% Triton X-100 and after two washes in PBS, cells were incubated in primary antibody against VGAT (diluted 1:400).

Related Products: vGAT Rabbit Polyclonal (Cat. #AB-N44)

Kline IV RH, Wiley RG (2008) Spinal mu-opioid receptor-expressing dorsal horn neurons: role in nociception and morphine antinociception. J Neurosci 28:904-913. doi: 10.1523/JNEUROSCI.4452-07.2008

Summary: The authors used Dermorphin-SAP (Cat. #IT-12) to investigate the function of spinal cord mu-opioid receptor (MOR)-expressing dorsal horn neurons in nociception and morphine analgesia. Rats were treated with 500 ng intrathecal injections of Dermorphin-SAP; 500 ng of Blank-SAP (Cat. #IT-21), and up to 1 µg of Saporin (Cat. #PR-01) were used as controls. The data indicate that MOR-expressing dorsal horn neurons are necessary for morphine action and play a role in nocifensive responses to persistent pain in the formalin test.

Related Products: Dermorphin-SAP / MOR-SAP (Cat. #IT-12), Blank-SAP (Cat. #IT-21), Saporin (Cat. #PR-01)

Lau T, Horschitz S, Berger S, Bartsch D, Schloss P (2008) Antidepressant-induced internalization of the serotonin transporter in serotonergic neurons. FASEB J 22(6):1702-1714. doi: 10.1096/fj.07-095471 PMID: 18216289

Related Products: Antibody to Serotonin Transporter (SERT, Cat. #AB-N09)

Dhaka A, Earley TJ, Watson J, Patapoutian A (2008) Visualizing cold spots: TRPM8-expressing sensory neurons and their projections. J Neurosci 28(3):566-575. doi: 10.1523/JNEUROSCI.3976-07.2008 PMID: 18199758

Related Products: Antibody to NK-1 Receptor (Cat. #AB-N04)

Kaur S, Junek A, Black MA, Semba K (2008) Effects of ibotenate and 192IgG-saporin lesions of the nucleus basalis magnocellularis/substantia innominata on spontaneous sleep and wake states and on recovery sleep after sleep deprivation in rats. J Neurosci 28:491-504. doi: 10.1523/JNEUROSCI.1585-07.2008

Objective: To examine the role of this area of the brain in several facets of sleep behavior.

Summary: The results suggest that cholinergic neurons and non-cholinergic neurons in the basal forebrain play different, but important roles in non-rapid eye movement sleep and EEG delta power after sleep loss. Non-cholinergic basal forebrain neurons inhibit delta waves, whereas cholinergic neurons promote wakefulness.

Usage: The caudal basal forebrain of rats was lesioned with 0.26-µg bilateral injections of 192-IgG-SAP.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Pang KCH, Jiao X, Servatius RJ (2008) Featured Article: Role of medial septal GABAergic neurons in learning and extinction: Effects of the novel GABA immunotoxin GAT1-SAP. Targeting Trends 9(1)

Related Products: GAT1-SAP (Cat. #IT-32)

Read the featured article in Targeting Trends.

Lo KW, Kogoy JM, Rasoul BA, King SM, Pfister KK (2007) Interaction of the DYNLT (TCTEX1/RP3) light chains and the intermediate chains reveals novel intersubunit regulation during assembly of the dynein complex. J Biol Chem 282(51):36871-36878. doi: 10.1074/jbc.M705991200 PMID: 17965411

Related Products: Antibody to Dynein Light Chain 3 (10A3-C8-A1) (Cat. #AB-V72), Antibody to Dynein Light Chain 1/3 (10F6-D1-D9) (Cat. #AB-V73), Antibody to Dynein Light Chain 1/3 (6C4-C12-F8) (Cat. #AB-V74), Antibody to Dynein Light Chain 1 (7A9-F4-H10) (Cat. #AB-V75)

Blanco-Centurion C, Gerashchenko D, Shiromani PJ (2007) Effects of saporin-induced lesions of three arousal populations on daily levels of sleep and wake. J Neurosci 27:14041-14048. doi: 10.1523/JNEUROSCI.3217-07.2007

Summary: Orexin neurons in the basal forebrain, tuberomammillary nucleus (TMN), and locus ceruleus (LC) are thought to regulate arousal. Rats were injected with two or three of the following targeted conjugates: anti-DBH-SAP (Cat. #IT-03), 0.25 µl bilateral injections of 1 µg/µl into the LC; orexin-SAP (Cat. #IT-20), 0.25 µl injection of 0.25 µg/µl into the TMN; 192-IgG-SAP (Cat. #IT-01), 3 µl injection of 2 µg/µl into the lateral ventricle. Small differences were observed in sleep architecture, but the data do not support the traditional hypothesis that these three areas of the brain are essential links in the control of wake levels.

Related Products: Anti-DBH-SAP (Cat. #IT-03), Orexin-B-SAP (Cat. #IT-20), 192-IgG-SAP (Cat. #IT-01)

Berdiev RK, Chepurnov SA, Veening JG, Chepurnova NE, van Luijtelaar G (2007) The role of the nucleus basalis of Meynert and reticular thalamic nucleus in pathogenesis of genetically determined absence epilepsy in rats: A lesion study. Brain Res 1185:266-274. doi: 10.1016/j.brainres.2007.09.010

Summary: Absence seizures due to epilepsy usually occur during passive behavior. This work investigated the role of the cholinergic nucleus basalis of Meynert (NB) and the reticular thalamic nucleus (RT) in these seizures. Rats received either 75 ng of 192-IgG-SAP (Cat. #IT-01) or the control, mouse IgG-SAP (Cat. #IT-18), into the NB and the RT. Loss of cholinergic neurons in the NB resulted in an increased number of spike-and-wave discharges, a marker for absence seizures.

Related Products: 192-IgG-SAP (Cat. #IT-01), Mouse IgG-SAP (Cat. #IT-18)