Voisin T, Gheziel N, El Samrout C, Martin J, Bradaia A, Iftinca M, Defaye M, Charron A, Abdullah NS, Changenot C, Gonzalez AA, Depluech A, Labit E, Saint-Laurent N, Staurengo-Ferrari L, Erdogan O, Tauber M, Loste A, Serhan N, Villa-Hernandez S, Chiu IM, Moqrich A, Altier C, Basso L, Gaudenzio N (2026) Skin inflammation and itch response are independently regulated by distinct nociceptor subsets. Immunity 59(5):1237-1252.e9. doi: 10.1016/j.immuni.2026.03.020 PMID: 41990747
Objective: To show the presence of two distinct and adaptive neuronal circuits that independently regulate allergic inflammation and itch in the skin in a model of allergic contact dermatitis (ACD).
Summary: To confirm the role of NP1 neurons in CHS-associated itch, the authors targeted IB4+ NP1 neurons using saporin-conjugated IB4. Neuronal loss was confirmed, as well as a partial depletion of IB4+ neurons and GFRα2+ neurons, but not TH+ neurons, in IB4-sap mice. Chemical depletion of IB4+ neurons led to a significant decrease in the number of ATF3+ neurons, little or no differences in immune response or pathology, but a significant reduction in scratching behavior.
Usage: Intrathecal injections were performed and mice received 5 μL of either IB4-saporin (IB4-SAP, 1.5μg/mouse; IT-10) or control saporin (650ng/mouse; PR-01) diluted in sterile PBS.
Related Products: IB4-SAP (Cat. #IT-10), Saporin (Cat. #PR-01)