Kozlíková M, Aukrust IKF, Rohlíčková M, Macháček M, Berg K, Weyergang A, Selbo PK (2026) Photochemical enhancement of PD-L1-SAP immunotoxin efficacy in non-small cell lung cancer cell lines. Front Immunol 17:1750003. doi: 10.3389/fimmu.2026.1750003 PMID: 41909645
Objective: To investigate photochemical internalization (PCI), a light–controlled endosomal escape technology, as a strategy to enhance intracellular delivery and efficacy of a PD–L1–targeted immunotoxin (anti–PD–L1–SAP).
Summary: Non–small cell lung cancer (NSCLC) cell lines with high (NCI–H1975) and low (A549) PD–L1 expression were subjected to PCI, resulting in a pronounced increase in cytotoxicity with picomolar potency (30 pM), while A549 cells required a higher dose (1000 pM) for a similar effect. PCI enhances delivery and activity of PD–L1–targeted biologics and may help overcome resistance mechanisms. Overall, PCI expands the therapeutic window of PD–L1–targeted immunotoxins and may complement current immunotherapies, supporting further preclinical evaluation in NSCLC.
Usage: Cells were then incubated with TPCS2a and anti-PD-L1-SAP (IT-45) for 18 h. The concentration of anti-PD-L1-SAP was 30, 100 and 1000 pM for NCI-H1975 cell line and 1000 pM for A549 cell line. BIgG-SAP (IT-74) was used as control.
Related Products: Anti-PD-L1-SAP (Cat. #IT-45), BIgG-SAP Mouse (Cat. #IT-74)