Landrigan J, Dwyer Z, Beauchamp S, Rodriguez R, Fortin T, Hayley S (2020) Quantum dot conjugated saporin activates microglia and induces selective substantia nigra degeneration. NeuroToxicology 76:153-161. doi: 10.1016/j.neuro.2019.11.007
Objective: To assess the impact of Quantum Dots (QDs) alone and QDs conjugated to Saporin, on substantia nigra microglia and dopamine neurons.
Summary: QDs might be a viable route for toxicant delivery and also have an added advantage of being fluorescently visible. Ultimately, we found SNc neurons to be exceptionally vulnerable to QD-saporin and suggest that this could be a novel targeted approach to model Parkinson’s Disease-like inflammatory pathology.
Usage: Biotin-labeled saporin chicken polyclonal was mixed with QDs coated with streptavidin. 2 μl of QDs (1 μM) were mixed with 2 μL of biotinylated saporin (56 μM) and 76 μL of phosphate buffer solution was added.